本文選題：人乳頭瘤病毒　切入點(diǎn)：外殼蛋白L1　出處：《三峽大學(xué)》2005年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目的:明確一種人乳頭瘤病毒(human papillomavirus,HPV)外殼蛋白L1的B細(xì)胞共同表位短肽作為HPV疫苗發(fā)展的可能性。 方法:通過(guò)對(duì)HPV-16、18、6和11型等幾種主要致病型別HPV外殼蛋白L1的三維結(jié)構(gòu)及其氨基酸序列的比較和分析,構(gòu)建HPV外殼蛋白L1的B細(xì)胞共同表位短肽疫苗,并以該短肽對(duì)動(dòng)物進(jìn)行免疫接種誘導(dǎo)抗短肽抗血清,再用酶聯(lián)免疫吸附測(cè)定(enzyme-linked immunosorbent assay, ELISA)、十二烷基磺酸鈉-聚丙酰胺凝膠電泳(SDS-PAGE)、免疫印跡(Western blot)及免疫組化(immunohistochemistry)等方法對(duì)短肽和抗短肽抗血清的免疫學(xué)特性進(jìn)行檢測(cè)。 結(jié)果:短肽與Furend`s完全佐劑合用免疫動(dòng)物可誘導(dǎo)高滴度的抗短肽抗血清,并且該抗血清能參與多型別HPV交叉中和反應(yīng)。 結(jié)論:HPV外殼蛋白L1的B細(xì)胞共同表位短肽可以作為HPV疫苗候選物。
[Abstract]:Aim: to identify the possibility of the development of a human papillomavirus (HPV) coat protein L1 B cell epitope short peptide as a HPV vaccine. Methods: by comparing and analyzing the three dimensional structure and amino acid sequence of HPV coat protein L1, the common epitope short peptide vaccine of HPV coat protein L1 was constructed. The anti-short peptide antiserum was induced by immunizing animals with the short peptide. The immunological properties of short peptide and anti-short peptide antiserum were detected by enzyme-linked immunosorbent assay, ELISAA, sodium 12 alkyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGEG) and immunohistochemical histochemistry. etc. Results: the combination of short peptide and Furend's complete adjuvant could induce high titer of anti short peptide antiserum, and the antiserum could participate in cross neutralization reaction of multi type HPV. Conclusion the B cell common epitope short peptide of 1: HPV coat protein L 1 can be used as a candidate for HPV vaccine.
【學(xué)位授予單位】：三峽大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類(lèi)號(hào)】：R392

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 袁有成,易學(xué)瑞,祖萍,張宜俊;新型佐劑及其與重組HBsAg結(jié)合物的理化性質(zhì)的研究[J];中國(guó)生物制品學(xué)雜志;2003年03期

2 楊英,李秀娟,孫祖s，

本文編號(hào)：1611956