本文選題：干細(xì)胞　切入點(diǎn)：移植　出處：《中國(guó)組織工程研究》2014年14期 　論文類(lèi)型：期刊論文

【摘要】：背景:神經(jīng)前體細(xì)胞的免疫原性各家研究結(jié)果不一,尤其是體內(nèi)移植后的機(jī)體免疫反應(yīng)模式需要進(jìn)一步研究。目的:體外觀察神經(jīng)前體細(xì)胞組成型及誘導(dǎo)型主要組織相容性抗原表達(dá)情況;體內(nèi)觀察神經(jīng)前體細(xì)胞移植入大鼠腦缺血組織后局部免疫細(xì)胞活化情況,探討神經(jīng)前體細(xì)胞的移植排斥可能性及模式。方法:自pCX-hrGFP ES-D3胚胎干細(xì)胞誘導(dǎo)分化神經(jīng)前體細(xì)胞,流式細(xì)胞術(shù)體外檢測(cè)主要組織相容性抗原Ⅰ,Ⅱ類(lèi)分子表達(dá)及γ-干擾素誘導(dǎo)前后表達(dá)變化。實(shí)驗(yàn)分3組,磷酸鹽緩沖液組、神經(jīng)前體細(xì)胞組分別于大腦中動(dòng)脈缺血大鼠模型造模后經(jīng)側(cè)腦室給予磷酸鹽緩沖液注射及神經(jīng)前體細(xì)胞移植,假手術(shù)組不造模。免疫組化法觀察紋狀區(qū)ED1+、CD4+、CD8+細(xì)胞浸潤(rùn)情況;淋巴細(xì)胞再刺激增殖實(shí)驗(yàn)觀測(cè)神經(jīng)前體細(xì)胞誘導(dǎo)移植大鼠頸部淋巴細(xì)胞的增殖指數(shù)。結(jié)果與結(jié)論:神經(jīng)前體細(xì)胞組成型高表達(dá)主要組織相容性抗原Ⅰ類(lèi)分子,幾乎不表達(dá)主要組織相容性抗原Ⅱ類(lèi)分子;經(jīng)γ-干擾素誘導(dǎo)后,主要組織相容性抗原Ⅰ類(lèi)分子進(jìn)一步上調(diào),主要組織相容性抗原Ⅱ類(lèi)分子亦有輕度上調(diào),提示神經(jīng)前體細(xì)胞有可能引起機(jī)體免疫反應(yīng)。移植實(shí)驗(yàn)表明,與假手術(shù)組相比,磷酸鹽緩沖液組及神經(jīng)前體細(xì)胞組均表現(xiàn)強(qiáng)烈的ED1+、CD4+、CD8+細(xì)胞浸潤(rùn)(P0.05),說(shuō)明腦缺血損傷本身能導(dǎo)致局部免疫細(xì)胞活化;神經(jīng)前體細(xì)胞組比磷酸鹽緩沖液組有更強(qiáng)的ED1+、CD4+細(xì)胞浸潤(rùn)(P0.05),提示神經(jīng)前體細(xì)胞移植可能導(dǎo)致局部免疫更進(jìn)一步活化,且以CD4+T細(xì)胞反應(yīng)為主。磷酸鹽緩沖液組及神經(jīng)前體細(xì)胞組神經(jīng)前體細(xì)胞誘導(dǎo)下的增殖指數(shù)值均較假手術(shù)組升高(P0.01),但前兩組增殖指數(shù)值比較差異無(wú)顯著性意義(P0.05),提示腦組織局部炎癥導(dǎo)致頸部淋巴細(xì)胞增殖性增加,而離體神經(jīng)前體細(xì)胞不足以單獨(dú)刺激致敏淋巴細(xì)胞增殖。
[Abstract]:Background: the results of immunogenicity of neural precursor cells are different. Especially the model of immune response after transplantation in vivo needs further study. Objective: to observe the formation of neural progenitor cells and the expression of inducible major histocompatibility antigens in vitro. The activation of local immune cells after transplantation of neural precursor cells into ischemic brain tissue of rats was observed in vivo, and the possibility and mode of transplantation rejection of neural precursor cells were investigated. Methods: neural precursor cells were induced to differentiate from pCX-hrGFP ES-D3 embryonic stem cells. In vitro flow cytometry was used to detect the expression of major histocompatibility antigens class 鈪，

本文編號(hào)：1611155