本文選題：T-2毒素　切入點(diǎn)：抗獨(dú)特型抗體　出處：《鄭州大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】：背景：T-2毒素屬于單端孢霉烯族化合物中的A族，，是該類化合物中毒性最強(qiáng)、污染水平較高的毒素之一，主要是由三線鐮刀菌，梨孢鐮刀菌，擬枝孢鐮刀菌和木賊鐮刀菌等霉菌產(chǎn)生。T-2毒素主要污染小麥、玉米、大麥、燕麥和黑麥等谷物及麥芽、啤酒和面包等農(nóng)產(chǎn)品。關(guān)于T-2毒素污染情況的報(bào)道，大多集中于對(duì)谷物和飼料的調(diào)查。據(jù)文獻(xiàn)報(bào)道，T-2毒素在各洲的谷物中廣泛存在。國(guó)內(nèi)報(bào)道在大骨節(jié)病區(qū)小麥、玉米及非病區(qū)小麥、大米中均有檢出。結(jié)合國(guó)外資料，認(rèn)為T-2毒素在全世界不同地區(qū)，對(duì)不同谷物均有一定程度的污染。 在動(dòng)物實(shí)驗(yàn)中，T-2毒素對(duì)動(dòng)物顯示了比較明確的免疫毒性及血液毒性，可抑制DNA、RNA及蛋白質(zhì)合成，誘導(dǎo)細(xì)胞凋亡，引起白細(xì)胞缺乏等。人類誤食了大量污染該類毒素的谷物后，除表現(xiàn)嘔吐、腹痛、腹瀉等急性癥狀外，可有白細(xì)胞缺乏，壞死性咽峽炎，骨髓發(fā)育不良，食管和胃嚴(yán)重壞死等，病死率高(死亡率高達(dá)50％-60％)。二戰(zhàn)期間前西伯利亞阿穆爾地區(qū)，數(shù)千人因進(jìn)食了被鐮刀菌污染的越冬小麥而發(fā)生食物中毒，在后來(lái)的產(chǎn)毒試驗(yàn)中，發(fā)現(xiàn)了高水平的T-2毒素；我國(guó)的安徽、河南兩省在1991年因洪澇災(zāi)害而引起霉變小麥中毒，當(dāng)時(shí)在采集的48份樣品中，47份均檢出T-2毒素，平均含量在255.9～671.6ppb之間，最高值可達(dá)1064.6ppb。此外，T-2毒素還與我國(guó)某些地區(qū)食管癌、克山病和大骨節(jié)病的高發(fā)病率有著密切的關(guān)系。近幾十年，T-2毒素還被作為生物戰(zhàn)劑用于戰(zhàn)爭(zhēng)中。 目前，檢測(cè)糧谷類食品和飼料中T-2毒素的方法主要有薄層色譜法(TLC)、液相色譜法(LC)、氣相色譜法(GC)、氣質(zhì)聯(lián)用(GC-MS)、酶聯(lián)免疫吸附測(cè)
[Abstract]:Background: T-2 toxin belongs to group A of monosporin compounds. It is one of the most toxic and highly polluted toxins in this kind of compounds. It is mainly composed of Fusarium triticum and Fusarium pirifera. Fungi such as Fusarium cyclosporum and Fusarium przewalskii mainly pollute cereals such as wheat, corn, barley, oats and rye, as well as agricultural products such as malt, beer and bread. Most of them focus on the investigation of grain and feed. According to the literature, it is reported that T-2 toxin is widely found in cereals of all continents. Domestic reports have been found in wheat, maize and wheat and rice in Kashin-Beck disease area. It is considered that T-2 toxin pollutes different grains in different regions of the world. In animal experiments, T-2 toxin showed specific immunotoxicity and blood toxicity to animals, which could inhibit the synthesis of DNA RNA and protein, induce cell apoptosis, cause leukocyte deficiency, etc. In addition to symptoms such as vomiting, abdominal pain, diarrhea and other acute symptoms, there may be leukopenia, necrotic spondylitis, dysplasia of bone marrow, severe necrosis of the esophagus and stomach, etc. The mortality rate is high (the mortality rate is as high as 50-60%). Before World War II, the Amour region of Siberia, Thousands of people suffered from food poisoning due to eating winter wheat contaminated by Fusarium, and a high level of T-2 toxin was found in subsequent toxicity tests. In 1991, two provinces of Anhui and Henan provinces in China were poisoned by mildew due to floods. T-2 toxin was detected in 47 of the 48 samples collected at that time, the average content was between 255.9 and 671.6 ppb, and the highest value was 1064.6 ppb. in addition, T-2 toxin was also detected in some regions of China. Keshan disease is closely related to the high incidence of Kaschin-Beck disease. T-2 toxin has been used as a biological warfare agent in recent decades. At present, the main methods to detect T-2 toxin in cereals and cereals are TLC, LC, GC, GC, GC-MSG, enzyme-linked immunosorbent assay (Elisa).
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 林超;王東旭;任洪林;柳增善;周玉;李巖松;閆東明;王里奇;盧士英;;抗大田軟海綿酸單抗獨(dú)特型抗體的制備及鑒定[J];食品科學(xué);2011年11期

相關(guān)碩士學(xué)位論文 前1條

1 薄智勇;伊維菌素抗獨(dú)特型和抗異型抗體的制備及鑒定[D];揚(yáng)州大學(xué);2010年

本文編號(hào)：1607750