本文選題：有機(jī)磷殺蟲(chóng)劑　切入點(diǎn)：G蛋白結(jié)合受體激酶-2　出處：《吉林大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

【摘要】： 研究有機(jī)磷類殺蟲(chóng)劑(OPs)對(duì)G蛋白結(jié)合受體激酶-2(GRK-2)介導(dǎo)的毒蕈堿乙酰膽堿m2受體(mAChR2)磷酸化的影響，進(jìn)一步揭示OPs可能存在的其他作用途徑。將純化的大鼠腦mAChR2，GRK-2，同位素磷32標(biāo)記的三磷酸腺苷([γ-P~(32)]ATP)與不同濃度的對(duì)氧磷(PO)、氧毒死蜱(CPO)和毒死蜱(CPF)共同保溫，聚丙烯酰胺凝膠電泳，凝膠片干燥后放射性自顯影檢測(cè)mAChR2磷酸化結(jié)果。將干燥凝膠片中放射性標(biāo)記的磷酸化mAChR2蛋白帶剪下，同位素液體閃爍計(jì)數(shù)器計(jì)數(shù)。CPO抑制大鼠腦mAChR2的磷酸化，其IC_(50)為70μmol／L；CPF也抑制mAChR2的磷酸化，而PO與對(duì)硫磷(PT)不抑制mAChR2的磷酸化。CPO和CPF并不抑制同樣由GRK2介導(dǎo)的β2腎上腺素受體(β2-AR)的磷酸化。CPO和CPF選擇性地抑制GRK-2介導(dǎo)的mAChR2磷酸化，，而PO和PT無(wú)此效應(yīng)。說(shuō)明某些有機(jī)磷類殺蟲(chóng)劑可能存在其它作用靶分子。
[Abstract]:To study the effect of organophosphorus insecticide (OPs) on phosphorylation of muscarinic acetylcholine m2 receptor mAChR2 mediated by G protein-binding receptor kinase-2 (GRK-2). To further reveal the possible role of OPs. The purified rat brain mAChR2GRK-2 and the isotopic phosphorus-32 labeled adenosine triphosphate ([緯 -Pn32] ATP) were incubated with different concentrations of p-phosphoric acid, chlorpyrifos, chlorpyrifos and chlorpyrifos, and polyacrylamide gel electrophoresis was used. The phosphorylation of mAChR2 was detected by autoradiography after drying. The phosphorylated mAChR2 protein band labeled in the dried gel was cut off, and the mAChR2 phosphorylation in rat brain was inhibited by isotope liquid scintillation counter count. Its ICP-50) also inhibited the phosphorylation of mAChR2, while PO and parathion did not inhibit the phosphorylation of mAChR2. CPO and CPF did not inhibit the phosphorylation of 尾 _ 2-adrenergic receptor (尾 _ 2-ARR), which was also mediated by GRK2, and CPF selectively inhibited mAChR2 phosphorylation mediated by GRK-2. However, PO and PT had no such effect, suggesting that some organophosphorus insecticides might have other target molecules.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R363

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本文編號(hào)：1604347