本文選題：流感病毒　切入點(diǎn)：神經(jīng)氨酸酶　出處：《湖南師范大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：流感是一種烈性傳染性疾病,每年造成很多人的死亡。DNA疫苗作為一種前景廣闊的疫苗已廣泛應(yīng)用于流感病毒預(yù)防研究。本論文分三個(gè)部分介紹我們?cè)贒NA疫苗研究方面的工作: Ⅰ.我們以前的研究表明NA、HA DNA疫苗在小鼠模型上誘導(dǎo)的免疫反應(yīng)可以提供抗流感病毒保護(hù),但是一般需要給小鼠接種兩次NA或HA DNA疫苗才能夠完全保護(hù)。為了簡(jiǎn)化免疫程序,本研究采用了一次免疫策略來(lái)檢測(cè)NA、HA DNA疫苗的免疫效果。我們用電穿孔的方法給小鼠接種一次NA DNA或HA DNA,檢測(cè)其保護(hù)小鼠的能力。BALB/c小鼠接種一次30μg NA、HA,在小鼠免疫后的第4周、第28周分別用致死量(40×LD_(50))流感病毒攻擊。實(shí)驗(yàn)表明接種一次30μg NA不但能在免疫后4周保護(hù)小鼠,而且在免疫后28周仍然能夠保護(hù)。接著,我們又對(duì)接種一次NA DNA進(jìn)行了劑量研究。實(shí)驗(yàn)表明,小鼠接種5μg NA就能在致死量流感病毒攻擊下存活,接種了低于這個(gè)劑量的小鼠在攻擊后1周全部死亡。最后,我們檢測(cè)了NA DNA在新生小鼠體內(nèi)的保護(hù)作用,結(jié)果發(fā)現(xiàn),一日齡和一周齡小鼠免疫了30μg NA后在致死量病毒攻擊下存活數(shù)量具有顯著的統(tǒng)計(jì)學(xué)意義。在以上實(shí)驗(yàn)中接種一次30μg HA的小鼠在攻擊后的存活數(shù)都沒(méi)有統(tǒng)計(jì)學(xué)意義。綜合以上實(shí)驗(yàn)結(jié)果,NA作為一種簡(jiǎn)單有效的DNA疫苗,對(duì)預(yù)防流感有積極意義。 Ⅱ.本部分旨在以小鼠為動(dòng)物模型,研究母體免疫流感病毒血凝素(hemagglutinin,HA)DNA疫苗對(duì)子代小鼠的保護(hù)作用。在本實(shí)驗(yàn)中,雌
[Abstract]:Influenza is a severe infectious disease. As a promising vaccine, DNA vaccine has been widely used in influenza virus prevention research. This paper introduces our work on DNA vaccine research in three parts:. I. Our previous studies have shown that the immune response induced by NAHA-HA DNA vaccine in mice can provide anti-influenza virus protection, but it usually takes twice to vaccinate mice with na or HA DNA to be fully protected. In this study, we used one time immunization strategy to detect the immune effect of NAA HA DNA vaccine. We used electroporation method to inoculate mice with na DNA or HA DNA once. The ability of protecting mice. BALB- c mice were inoculated with 30 渭 g NAA at 4 weeks after immunization. The experiment showed that inoculation with 30 渭 g na could protect mice not only 4 weeks after immunization, but also 28 weeks after immunization. We also carried out a dose study of na DNA inoculation. The experiment showed that mice inoculated with 5 渭 g na could survive the lethal influenza virus attack, and all the mice inoculated with less than this dose died one week after the attack. We examined the protective effect of na DNA in newborn mice and found that, The number of mice immunized with 30 渭 g na for one day and one week after inoculation with lethal viral attack was statistically significant. There was no significant difference in the survival rate of mice inoculated with 30 渭 g HA at a time in the above experiment. To sum up the above results, na as a simple and effective DNA vaccine, It has positive significance for the prevention of influenza. 鈪，

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