本文選題：糖尿病　切入點：心血瘀阻　出處：《湖南中醫(yī)藥大學》2006年碩士論文　論文類型：學位論文

【摘要】： 目的:建立2型糖尿病心血瘀阻證的病證結(jié)合的實驗動物模型,使糖尿病并發(fā)癥的中醫(yī)證型動物模型規(guī)范化、標準化,為臨床糖尿病心血管并發(fā)癥的有效治療和中藥新藥開發(fā)創(chuàng)造良好條件。 方法:將46只清潔級wistar大鼠常規(guī)飼養(yǎng)一周后,隨機抽取10只為正常對照組,其余36只為造模組。造模組喂以高脂飼料4周后,再隨機抽取12只為心血瘀阻證造模組(心血瘀阻證模型組),其余24只注射少劑量的鏈脲佐菌素行糖尿病造模,成模大鼠按血糖、體重分為兩組,隨機抽取一組為糖尿病模型組(糖尿病組),剩下一組利用強的松龍和腎上腺素行血瘀證造模,從而誘導出糖尿“病”心血瘀阻“證”病證結(jié)合的大鼠模型(糖尿病心血瘀阻證組)。 結(jié)果: (1)一般狀況:糖尿病心血瘀阻證組(糖尿病心血瘀證組)大鼠造模后出現(xiàn)精神萎糜,倦怠懶動,消瘦、大便量多、稀,小便稍多,舌上可見瘀點、甚可見瘀斑等癥狀為造模成功。 (2)糖尿病心血瘀證組與其它三組比較,血糖均有增高,差異具有顯著性(p＜0.01或p＜0.05)。 (3)糖尿病心血瘀證組的心臟濕重與體重的比值和心電圖結(jié)果與其他三組比較,差異具有顯著性(p＜0.01或p＜0.05)。 (4)糖尿病心血瘀證組血液流變學多項主要指標值較其它三組比較,均有升高,差異具有顯著性(p＜0.01或p＜0.05)。 (5)糖尿病心血瘀證組的球結(jié)膜管袢形態(tài)積分和流態(tài)積分與其它三組比較,差異具有顯著性(p＜0.01或p＜0.05);而周態(tài)積分無異常(p＞0.05)。 (6)糖尿病心血瘀證組與其它三組比較,總膽固醇、甘油三脂有明顯升高、高密度脂蛋白有明顯降低,差異具有顯著性(p＜0.01或p＜0.05)。 (7)光鏡下,糖尿病心血瘀證組心肌細胞有炎癥細胞浸潤,細胞壞死和肌纖維斷裂。 (8)光鏡下,糖尿病心血瘀證組的主動脈有動脈斑塊。 結(jié)論:1.應用傳統(tǒng)的糖尿病造模方法與糖尿病并發(fā)癥病證特點和糖尿病發(fā)病的病因病理相結(jié)合而研制2型糖尿病心血瘀阻證的病證結(jié)合的動物模型是切實可行的。 2.在糖尿病模型的基礎(chǔ)上再造血瘀證模型,從而誘導出糖尿病心血瘀證模型的造模方法,創(chuàng)立出糖尿“病”和心血瘀“證”相結(jié)合的病證結(jié)合的動物模型,此方法可推廣。
[Abstract]:Objective: to establish an experimental animal model of the combination of disease and syndrome of type 2 diabetes mellitus with heart blood stasis syndrome, and to standardize and standardize the animal model of TCM syndrome of diabetic complications. To provide a good condition for the effective treatment of cardiovascular complications of diabetes mellitus and the development of new drugs of traditional Chinese medicine. Methods: after a week of routine feeding of 46 clean grade wistar rats, 10 rats were randomly selected as normal control group and the remaining 36 rats as model group. The model group was fed with high fat diet for 4 weeks. Twelve rats were randomly selected as heart blood stasis syndrome model group (heart blood stasis syndrome model group), the remaining 24 rats were injected with low dose streptozotocin to make diabetes model. The model rats were divided into two groups according to blood sugar and body weight. One group was randomly selected as diabetic model group (diabetes group), and the remaining group was treated with prednisolone and epinephrine to make model with blood stasis syndrome. Thus, the rat model of diabetes "disease" and "heart blood stasis" syndrome combined with disease and syndromes was induced. Results:. (1) General condition: the rats of diabetic heart blood stasis syndrome group (diabetes heart blood stasis syndrome group) appeared mental wilting, languid and lazy, wasting, defecation amount, dilute, urination a little more, blood stasis on the tongue, It can be seen that ecchymosis and other symptoms for successful modeling. (2) compared with the other three groups, the blood glucose of the diabetic heart and blood stasis syndrome group was higher than that of the other three groups, and the difference was significant (p < 0.01 or p < 0.05). (3) compared with the other three groups, the ratio of heart dampness weight to body weight and electrocardiogram in the diabetic heart and blood stasis group were significantly different from those of the other three groups (p < 0.01 or p < 0.05). (4) compared with the other three groups, the hemorheology index of the diabetes heart and blood stasis syndrome group was higher than that of the other three groups, and the difference was significant (p < 0.01 or p < 0.05). (5) compared with the other three groups, the morphological integral and the flow integral of the bulbar conjunctiva in the diabetic heart and blood stasis syndrome group showed significant difference (p < 0.01 or p < 0.05), but the pericardial integral was not abnormal (p > 0.05). Compared with the other three groups, the total cholesterol, triglyceride and high density lipoprotein were significantly increased in the diabetic heart and blood stasis group (P < 0.01 or p < 0.05). Under light microscope, there were inflammatory cell infiltration, cell necrosis and muscle fiber breakage in diabetic heart and blood stasis group. Under light microscope, aortic plaques were found in diabetic heart and blood stasis group. Conclusion\\\. 2. Based on the model of diabetes mellitus, the model of blood stasis syndrome was reconstituted, and the animal model of the combination of diabetes "disease" and "heart blood stasis" was established, which can be popularized.
【學位授予單位】：湖南中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R259;R-332

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