本文選題：SARS冠狀病毒　切入點：基因組　出處：《浙江大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：2003年3月,一種新的冠狀病毒(CoV)—SARS冠狀病毒被確認(rèn)為引起SARS(severe acute respiratory syndrome)的病原。在本研究中,我們獲取了10株直接來源SARS病人 糞便或咽拭子樣本及其細(xì)胞傳代培養(yǎng)物的SARS-CoV全基因組序列,并會同公共數(shù)據(jù) 庫中的115株SARS-CoV基因組序列進(jìn)行了系統(tǒng)地比較分析。以GZ02序列為參照,發(fā) 現(xiàn)266個在2個以上基因組中同時存在的單核苷酸多態(tài)(SNP)位點。這些多態(tài)位點在 SARS-CoV基因組中呈偏態(tài)分布。同時發(fā)現(xiàn)了18種主要的缺失類型和2種主要的插入 類型,定義了2種與發(fā)病時期及致死率相關(guān)的基因型。S蛋白在發(fā)病早期受到最大的正 向選擇壓力,然后逐步穩(wěn)定下來。我們應(yīng)用鄰位連接法(neighbor-joining)構(gòu)建了125株 SARS-CoV的親緣樹。通過來源于同一病人的直接取樣樣本及其細(xì)胞傳代培養(yǎng)物的 SARS-CoV基因組序列對比分析發(fā)現(xiàn),在細(xì)胞培養(yǎng)過程中SARS-CoV基因組序列幾乎 不會產(chǎn)生突變,但取樣時間策略將會影響這一過程。推測這種現(xiàn)象有可能是由于細(xì)胞傳 代培養(yǎng)過程會選擇出某種優(yōu)勢株病毒造成的。
[Abstract]:In March 2003, a new coronavirus, CoV-SARS-CoV, was identified as causing SARS(severe. Acute respiratory syndrome. In this study, we obtained 10 direct source SARS patients. SARS-CoV genome sequence of fecal or pharyngeal swab samples and their cell passage cultures, and in conjunction with common data. The genomic sequences of 115 strains of SARS-CoV in the library were compared and analyzed systematically. There are 266 single nucleotide polymorphism (SNPs) loci in more than 2 genomes. In the SARS-CoV genome, 18 major deletion types and 2 major insertions were found. The two genotypes associated with the onset and mortality were defined as the most positive in the early stage of the disease. The selection pressure, and then gradually stabilized. We built 125 strains using the neighbor connection method (Nighbor-joining). A phylogenetic tree of SARS-CoV. Derived from a direct sample of the same patient and its cell passage culture. The comparative analysis of SARS-CoV genome sequence showed that the SARS-CoV genome sequence was almost in the process of cell culture. There is no mutation, but sampling time strategy will affect the process. It is assumed that this phenomenon may be due to cell transmission. Generation culture will result in the selection of a dominant strain of the virus.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R373

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 岳俊杰,汪莉,王月蘭,李北平,梁龍,俞煒源,黃培堂;SARS病毒S蛋白三維結(jié)構(gòu)預(yù)測[J];生物技術(shù)通訊;2003年05期

2 孟夏,胡放,邱祺宏,葉迅,梁e，

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