本文關鍵詞： 毛發(fā)生長發(fā)育異常 動物模型 ICR小鼠 C57BL/6小鼠 基因芯片 基因表達譜　出處：《第二軍醫(yī)大學》2006年博士論文　論文類型：學位論文

【摘要】：本研究從給藥劑量、給藥時間、性狀有無遺傳性幾個方面進行研究,建立了兩種品系小鼠毛發(fā)生長發(fā)育異常的動物模型。并從仔鼠毛發(fā)的生長及分布情況、毛發(fā)類型、毛發(fā)長度、毛發(fā)直徑及毛發(fā)密度幾個方面對各組仔鼠的毛發(fā)性狀進行了描述。10mg/kg組仔鼠在給藥期肉眼未見毛發(fā)生長。在此基礎上,用小鼠全基因組芯片對接觸藥物組仔鼠及對照組仔鼠皮膚毛囊組織的基因表達譜進行了分析,發(fā)現(xiàn)大部分編碼構成毛發(fā)的蛋白質(zhì)的相應基因(Krt及Krtap)在給藥組仔鼠的皮膚毛囊組織中的表達明顯下調(diào),而且參與Krt及Krtap表達調(diào)控的基因也發(fā)生了明顯變化。在此基礎上對CK-794誘發(fā)的毛發(fā)生長發(fā)育異�？赡艿臋C制進行初步分析。
[Abstract]:In this study, animal models of abnormal hair growth and development in two strains of mice were established from the aspects of dosage, time and heredity of administration, and the growth and distribution of hair and hair types in two strains of mice were also studied. Hair length, hair diameter and hair density were used to describe the hair characteristics of rats in each group. 10 mg / kg group had no hair growth in the naked eye during the administration period. The gene expression profiles of the skin hair follicles of the mice exposed to the drug and the control group were analyzed by using the whole genome microarray of mice. It was found that the expression of most of the corresponding genes, Krt and Krtapin, which encodes the proteins that constitute hair, was significantly down-regulated in the skin hair follicles of the rats in the administration group. Moreover, the genes involved in the regulation of Krt and Krtap expression also changed obviously. The possible mechanism of hair growth and development abnormality induced by CK-794 was analyzed.
【學位授予單位】：第二軍醫(yī)大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R-332

【共引文獻】

相關碩士學位論文 前1條

1 韓勇;不同培養(yǎng)基、藥物對體外人游離毛囊生長的影響[D];揚州大學;2007年

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本文編號：1553501