本文關鍵詞： 霍亂弧菌腸毒素B亞單位 大腸桿菌 雙歧桿菌 基因克隆 蛋白表達　出處：《第一軍醫(yī)大學》2006年碩士論文　論文類型：學位論文

【摘要】：研究背景： 霍亂(Cholera)是一種由霍亂弧菌(Vibrio cholerae)引起的烈性傳染病，一直以來都是各國傳染病防治的重點，免疫接種是預防霍亂的最有效措施。以前的注射用疫苗不同程度存在著各種缺點，研究也顯示針對霍亂這種腸道傳染病的疫苗，通過口服途徑給予的效果優(yōu)于其他任何胃腸外給予途徑，因為腸道粘膜免疫在霍亂的免疫預防中發(fā)揮著主要作用。因此，開發(fā)安全、方便、價廉的口服疫苗成為霍亂免疫預防研究的熱點，而以霍亂弧菌腸毒素(Cholera toxin，CT)為基礎的基因重組口服疫苗成為霍亂疫苗開發(fā)的主要方向。CT是霍亂弧菌主要的致病因子，它是分子量為84KD的腸毒素，由1個A亞單位(Cholera toxin Asubunit，CTA)和5個B亞單位(Cholera toxin B subunit，CTB)形成的五聚體共價連接而成，CTA為毒性亞單位，CTB為無毒的受體結合亞單位，后者是霍亂的主要保護性抗原。口服CTB可以刺激機體產(chǎn)生保護性粘膜抗體和血清抗體，發(fā)揮霍亂防治作用。此外，CTB還是一種很好的粘膜免疫佐劑，用于強化多糖、小肽等半抗原的免疫原性，與其他不相關抗原共同使用時，可以促進誘導其他抗原的粘膜免疫反應，，用于更廣泛的人類疫苗的發(fā)展。所以，為充分發(fā)揮CTB的免疫特性作用，探索CTB在各種宿主表達的研究日益增多，如細菌、植物、
[Abstract]:Research background:
Cholera (Cholera) is a kind of Vibrio cholerae (Vibrio cholerae) caused by infectious diseases, has been the focus of the prevention and treatment of infectious diseases, vaccination is the most effective measure for the prevention of cholera. Previous vaccines had many defects in different degrees. This study also showed that for cholera intestinal infectious disease vaccine that effect was given via the oral route is better than any other way for parenteral administration, intestinal mucosal immunity plays a major role in the immune prevention of cholera. Therefore, the development of safe, convenient, inexpensive oral disease prevention research has become the focus of vaccine immunity to cholera, cholera toxin (Cholera toxin CT) gene recombinant oral vaccine based cholera vaccine development has become the main direction of.CT is the main pathogenic factor of Vibrio cholerae, which is a molecular weight of 84KD enterotoxin, composed of 1 A subunits (Cholera to Xin Asubunit, CTA) and 5 B (Cholera toxin B subunit subunit, CTB) five dimer formation of covalent connection, CTA is the toxic subunit, CTB is a non-toxic receptor binding subunit, which is the major protective antigen of cholera. Oral CTB can stimulate the body to produce protective antibody and mucosa serum antibodies play a protective role for cholera. In addition, CTB is also a very good immunologic adjuvant, to enhance the immunogenicity of polysaccharides, small peptide hapten, use with other incorrelate antigen, inducing other antigens can promote mucosal immune response, for the wider human vaccine development so, to give full play to the role of the immune characteristics of CTB, to explore the expression of CTB in various host has increased, such as bacteria, plant,

【學位授予單位】：第一軍醫(yī)大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R378

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本文編號：1541941