本文關(guān)鍵詞： MAGE-A1 腫瘤 噬菌體抗體庫 單鏈抗體 全人抗體 親和常數(shù)　出處：《南京醫(yī)科大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：MAGE-Al為MAGE(melanoma associated antigen)家族中的一員,在多種腫瘤組織均有不同程度表達(dá),但在正常組織中除睪丸和胎盤外均不表達(dá)。因此,MAGE-Al抗原有可能作為腫瘤免疫治療的靶點(diǎn)。免疫治療是繼手術(shù)、化療、放療之后的第四種腫瘤治療模式,但是鼠源性單克隆抗體對人體有免疫原性,既會產(chǎn)生人抗鼠抗體誘發(fā)過敏反應(yīng),又不能有效激活人體的生物效應(yīng)功能,在人體內(nèi)的半衰期也較短。鼠源性抗體的這些自身局限性極大地限制了其作為治療制劑在人體內(nèi)的應(yīng)用。1991年Winter等創(chuàng)立的噬菌體抗體庫技術(shù),以大腸桿菌作為宿主,絲狀噬菌體作為表達(dá)和展示的載體,使得抗體以及編碼抗體的DNA,共同存在于宿主菌中。噬菌體抗體庫技術(shù)使得產(chǎn)生全人源抗體成為可能。全人源噬菌體抗體庫用細(xì)菌克隆取代B細(xì)胞克隆表達(dá)抗體,不經(jīng)細(xì)胞融合,甚至不經(jīng)免疫,可以制備針對任何抗原的全人源抗體,對人體沒有免疫原性,不會引起過敏反應(yīng),可直接用于人體內(nèi)。本課題在參照國內(nèi)外相關(guān)研究的基礎(chǔ)上,以期從全人源單鏈?zhǔn)删w抗體庫中篩選出特異性抗MAGE-Al單鏈抗體,為腫瘤的免疫治療提供技術(shù)儲備。 研究目的 1.應(yīng)用噬菌體抗體庫技術(shù),篩選出全人源抗MAGE-Al噬菌體抗體,鑒定其免疫活性。
[Abstract]:MAGE-Al is a member of MAGE(melanoma associated antigen. it is expressed in various tumor tissues, but not in normal tissues except testis and placenta. Therefore, MAGE-Al antigen may be used as a target of tumor immunotherapy. There are 4th kinds of tumor treatment modes after chemotherapy and radiotherapy, but mouse monoclonal antibody has immunogenicity to human body, it can not only produce human anti-mouse antibody to induce allergic reaction, but also can not effectively activate the biological effect function of human body. In 1991, Winter and other phage antibody library techniques were created to use Escherichia coli as host. Filamentous phage as expression and display vector, The technique of phage antibody library makes it possible to produce whole human antibody. The whole human phage antibody library uses bacterial clone instead of B cell clone to express antibody without cell fusion. Even without immunity, the whole human antibody against any antigen can be prepared, and it has no immunogenicity to the human body and will not cause allergic reaction, so it can be directly used in human body. The aim of this study was to screen the specific anti MAGE-Al single chain antibody from the whole human single chain phage antibody library and to provide a technical reserve for tumor immunotherapy. Research purpose. 1. The whole human anti MAGE-Al phage antibody was screened by phage antibody library technique and its immunological activity was identified.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R392

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本文編號：1510741