本文關(guān)鍵詞： 人類皰疹病毒6型 脂筏 膽固醇 感染　出處：《吉林大學(xué)》2006年博士論文　論文類型：學(xué)位論文

【摘要】：為了探討脂筏(lipid raft)在人類皰疹病毒6型(HHV-6)的裝配與在病毒感染中的作用,用HHV-6 GS株感染HSB2細(xì)胞,用非離子去污劑Triton X-100提取脂筏成分,利用Western Blot分析HHV-6包膜糖蛋白與脂筏的相關(guān)性。并用免疫熒光雙標(biāo)記的方法,從分子共定位的角度研究HHV-6糖蛋白B(gB)與GPI錨固蛋白CD59分子以及神經(jīng)節(jié)苷脂GM1分子之間的表達(dá)與分布關(guān)系。結(jié)果顯示,HHV-6包膜糖蛋白B、H、L、Q1和Q2(gB、gH、gL、gQ1和gQ2)分布在脂筏部位。激光共聚焦顯微鏡可觀察到CD59分子及GM1均與HHV-6包膜糖蛋白B有著相同的分布。另外,在純化的HHV-6顆粒中檢測到了脂筏的指示劑GM1分子。 為了探討構(gòu)成病毒包膜的脂筏成分在病毒感染中的作用,用甲基-β-環(huán)糊精(MβCD)從HHV-6顆粒中去除脂筏的主要組成成分膽固醇,結(jié)果顯示,病毒的感染呈劑量依賴性降低,而補(bǔ)充外源性膽固醇后可恢復(fù)其感染率。用膽固醇提取劑MβCD處理后,HHV-6病毒顆粒雖然能夠與靶細(xì)胞結(jié)合但不能進(jìn)入到細(xì)胞內(nèi)部。 本研究的結(jié)果揭示了脂筏提供HHV-6裝配的平臺,且病毒包膜中的脂筏成分在病毒的感染過程中起著重要的作用。
[Abstract]:In order to investigate the role of lipid raft in the assembly of human herpesvirus 6 (HHV-6) and its role in viral infection, HSB2 cells were infected with HHV-6 GS strain. The components of lipid raft were extracted by non-ionic detergent Triton X-100. The correlation between HHV-6 envelope glycoprotein and lipid raft was analyzed by Western Blot, and immunofluorescence double labeling method was used. The expression and distribution of HHV-6 glycoprotein (HHV-6), GPI anchored protein (CD59) and ganglioside (GM1) molecules were studied from the point of molecular co-localization. The HHV-6 envelope glycoprotein (HHV-6) was found to be Q1 and Q2GBG / gH1, respectively. GQ1 and gQ2) were distributed in lipid rafts. The same distribution of CD59 and GM1 was observed by confocal laser microscopy with HHV-6 coated glycoprotein B. The indicator GM1 molecule of lipid raft was detected in purified HHV-6 particles. In order to investigate the role of lipid raft components that form viral envelope in viral infection, cholesterol was removed from HHV-6 granules by methyl 尾 -cyclodextrin M 尾 CD-CD2. The results showed that cholesterol was the main component of lipid raft. The infection rate of the virus was decreased in a dose-dependent manner, and the infection rate could be recovered after supplementation of exogenous cholesterol, which was treated with cholesterol extractant M 尾 CD. Although HHV-6 virus particles can bind to target cells, they cannot enter the cells. The results of this study revealed that lipid rafts provide a platform for HHV-6 assembly, and the lipid rafts in viral envelope play an important role in the process of virus infection.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：R373

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 吳寒宇;黃紅蘭;;脂筏及其在病原生物感染中的作用概述[J];中國病原生物學(xué)雜志;2011年06期

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本文編號：1493807