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  本文關(guān)鍵詞： 納米金免疫層析試條 光電檢測 小波變換 模糊聚類識別 定量測試　出處：《福州大學(xué)》2005年博士論文　論文類型：學(xué)位論文

【摘要】：免疫測定是基礎(chǔ)研究和臨床檢測中應(yīng)用廣泛、分析敏感的一種技術(shù),遍及醫(yī)學(xué)檢驗的各個領(lǐng)域。隨著免疫測定技術(shù)的日新月異,許多新技術(shù)取代傳統(tǒng)的實驗方法,納米金免疫層析試條就是其中一種。 納米金免疫層析試條具有檢測效率高、方法簡便、一步完成、無污染、試劑穩(wěn)定、適用于單人份測定等特點,已受到臨床檢驗界的極大關(guān)注,并發(fā)展成為臨床檢驗的前沿領(lǐng)域。 但是,目前納米金免疫層析試條主要用于定性或半定量的快速免疫檢測方法中,使試條的臨床應(yīng)用范圍受到了限制。因此,納米金免疫層析試條定量測試的研究具有重要的理論意義與應(yīng)用價值。 本文從理論上對納米金免疫層析試條定量測試機理進行研究,以試條光譜峰特性曲線為研究重點,將納米金免疫層析法與以光纖傳感為核心的光電檢測技術(shù)以及現(xiàn)代信息處理技術(shù)相結(jié)合,提出了納米金免疫層析試條智能定量測試的新概念、新思路,從而實現(xiàn)了對納米金免疫層析試條準(zhǔn)確地定量測試,為臨床免疫測定提供一種新的、有效的檢測手段。本文主要進行以下研究工作: (1) 探討納米金免疫層析試條定量測試機理和光譜峰信號的基本特點,深入分析對定量測試造成影響的各種干擾因素,如生化噪聲、光噪聲、電噪聲等,建立理想光譜峰曲線的數(shù)學(xué)表達式,研究適合納米金免疫層析試條智能定量測試的新機理。 (2) 將小波變換、模糊聚類識別和小波神經(jīng)網(wǎng)絡(luò)等技術(shù)引入納米金免疫層析試條定量檢測中,完成對試條邊緣特征提取、噪聲信號濾除和曲線擬合等,使現(xiàn)代信息處理技術(shù)對納米金免疫層析試條信號的分析從理論研究走向?qū)嶋H應(yīng)用。本文提出基于現(xiàn)代信息處理技術(shù)的納米金免疫層析試條智能定量測試新方法,拓展了現(xiàn)代信息處理技術(shù)在生物醫(yī)學(xué)領(lǐng)域的應(yīng)用。 (3) 根據(jù)朗伯—比爾定律和互補光原理,利用光電檢測系統(tǒng)將納米金免
[Abstract]:Immunoassay is widely used in basic research and clinical detection. It is a sensitive analytical technique, which is widely used in various fields of medical testing. With the rapid development of immunoassay technology. Many new technologies replace the traditional experimental methods, one of which is the nanocrystalline gold immunochromatographic strip. The nanocrystalline gold immunochromatographic strip has the characteristics of high detection efficiency, simple method, one-step completion, no pollution, stable reagent, suitable for single determination, and has been paid great attention to by the clinical laboratory. And has developed into the frontier field of clinical laboratory. However, at present, nanocrystalline gold immunochromatographic strips are mainly used in qualitative or semi-quantitative rapid immunoassay, which limits the clinical application of the strips. The study of gold nanoparticles immunochromatographic strip quantitative test has important theoretical significance and application value. In this paper, the mechanism of quantitative measurement of nanocrystalline gold immunochromatographic strip was studied theoretically, with the focus on the characteristic curve of the spectral peak of the strip. Combining nano-gold immunochromatography with optoelectronic detection technology based on optical fiber sensor and modern information processing technology, a new concept and new idea of intelligent quantitative test of nano-gold immunochromatographic strip are put forward. Thus, the gold nanoparticles immunochromatographic strip is tested accurately and quantitatively, which provides a new and effective method for clinical immunoassay. The main work of this paper is as follows: 1) to discuss the mechanism of quantitative measurement and the basic characteristics of spectral peak signal in nanoscale gold immunochromatographic strip, and to analyze in depth various interference factors, such as biochemical noise, light noise, electric noise and so on. The mathematical expression of the ideal spectral peak curve was established to study the new mechanism suitable for intelligent quantitative testing of nanocrystalline gold immunochromatographic strips. 2) the wavelet transform, fuzzy clustering recognition and wavelet neural network are introduced into the quantitative detection of the strip of nanoscale gold immunochromatography. The edge feature extraction, noise signal filtering and curve fitting are completed. This paper presents a new intelligent quantitative test method based on modern information processing technology to analyze the signal of nano-gold immunochromatographic strip from theoretical research to practical application. The application of modern information processing technology in biomedical field is expanded. According to Lambert-Beer 's law and complementary light principle, nanocrystalline gold can be eliminated by photoelectric detection system.
【學(xué)位授予單位】：福州大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R392

【引證文獻】

相關(guān)期刊論文 前4條

1 姜海燕;杜民;;小波變換在基于圖像處理的金免疫層析測定中的應(yīng)用[J];福州大學(xué)學(xué)報(自然科學(xué)版);2011年02期

2 王洋;蔣凱;王萍;韓紀(jì)昱;;基于光源調(diào)制技術(shù)的金標(biāo)試紙條定量分析儀器[J];電子技術(shù)應(yīng)用;2012年12期

3 趙晶;姜海燕;杜民;;毒品膠體金試劑快速測試儀的研制和應(yīng)用[J];生物醫(yī)學(xué)工程研究;2007年04期

4 韓達;;模糊PID復(fù)合控制[J];數(shù)字技術(shù)與應(yīng)用;2011年03期

相關(guān)碩士學(xué)位論文 前2條

1 付海霞;免疫層析快速檢測技術(shù)的研究[D];浙江大學(xué);2008年

2 王洋;高精度金標(biāo)試紙讀數(shù)儀的設(shè)計與開發(fā)[D];浙江大學(xué);2013年

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本文編號：1488657