本文關(guān)鍵詞： 甲醛 神經(jīng)毒性 Motris水迷宮 氧化損傷 NMDA受體　出處：《華中師范大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】：甲醛(formaldehyde,FA)是一種常見的裝修型化學(xué)性室內(nèi)空氣污染物,也是一種內(nèi)源性有機(jī)化合物。室內(nèi)空氣甲醛對人體健康的影響涉及:呼吸道和眼部刺激作用、致敏作用和免疫毒性、遺傳毒性和癌癥、肺功能損害、神經(jīng)行為改變等諸多方面。本課題對氣態(tài)甲醛對中樞神經(jīng)的毒性效應(yīng)進(jìn)行了研究,并對其可能機(jī)制進(jìn)行了探討。 采用濃度為0mg/m~3、0.5mg/m~3、1.0mg/m~3和3.0mg/m~3氣態(tài)甲醛對昆明小鼠進(jìn)行吸入式染毒(6h×7d),同時(shí)進(jìn)行Morris水迷宮實(shí)驗(yàn)(包括定位航行實(shí)驗(yàn)和空間探索實(shí)驗(yàn))檢測小鼠空間學(xué)習(xí)與記憶的能力,并在染毒結(jié)束后進(jìn)行腦組織氧化損傷水平的測定。另外采用3.0mg/m~3的氣態(tài)甲醛對BALB/C小鼠進(jìn)行吸入式染毒(6h×7d),染毒結(jié)束后立即取前腦組織,應(yīng)用半定量RT-PCR方法檢測其N—甲基-D-天冬氨酸(NMDA)受體亞基Grinl,Grin2a,Grin2b基因mRNA的表達(dá)水平。 結(jié)果顯示,定位航行實(shí)驗(yàn)各組小鼠的逃逸潛伏期均隨實(shí)驗(yàn)天數(shù)的增加而下降,實(shí)驗(yàn)天數(shù)(F=17.83,P0.01)和不同濃度的甲醛吸入(F=8.84,P0.01)對逃逸潛伏期均有極顯著影響,但二者沒有交互作用。對不同濃度甲醛吸入組采用新復(fù)極差法進(jìn)行多重比較,結(jié)果顯示0mg/m~3甲醛吸入組與0.5mg/m~3、1.0mg/m~3甲醛吸入組之間無顯著差異,與3.0mg/m~3甲醛吸入組之間有極顯著差異(P0.01)。 空間探索實(shí)驗(yàn)中0mg/m~3和0.5mg/m~3甲醛吸入組小鼠均在平臺所在象限的游泳時(shí)間較長,在其它象限的游泳時(shí)間相對較短。0mg/m~3甲醛吸入小鼠在平臺象限(NE)的游泳時(shí)間與SE象限的游泳時(shí)間相比存在顯著差異(P0.05),與NW、SW象限的游泳時(shí)間之間則存在極顯著差異(P0.01)。0.5mg/m~3甲醛吸入組小鼠在NE象限的游泳時(shí)間與其它三個(gè)象限相比均有極顯著差異(P0.01),并且其在NE象限的游泳時(shí)間顯著長于對照組小鼠(P0.01)。在3.0mg/m~3甲醛吸入組中,小鼠在SE象限的游泳時(shí)間最長,在平臺象限的游泳時(shí)間與NE和SW兩象限相差不大。 氧化損傷水平檢測中,1.0mg/m~3和3.0mg/m~3甲醛吸入小鼠的丙二醛(MDA)含量與對照組相比極顯著升高(P0.01),3.0mg/m~3甲醛吸入組與對照相比,其谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)活力均極顯著降低(P0.01)。 NMDA受體的mRNA表達(dá)水平變化為:甲醛吸入組與對照組相比Grinl的表達(dá)顯著上調(diào)(P0.05),Grin2b亞基的表達(dá)水平極顯著上升(P0.01),Grin2a基因表達(dá)極顯著下調(diào)(P0.01)。
[Abstract]:Formaldehyde formaldehydefa is a common chemical indoor air pollutant and an endogenous organic compound. The effects of formaldehyde in indoor air on human health include respiratory tract and eye irritation, sensitization and immunotoxicity. In this paper, the toxic effect of gaseous formaldehyde on central nervous system was studied and its possible mechanism was discussed. Kunming mice were exposed to inhaled formaldehyde at concentrations of 0 mg / m ~ (3) 0 mg / m ~ (3) 0 mg / m ~ (3) and 3.0 mg / m ~ (-3) gaseous formaldehyde for 6 h 脳 7 d ~ (-1) respectively. Meanwhile, Morris water maze test (including positioning navigation experiment and space exploration experiment) was performed to examine the ability of spatial learning and memory in mice. The level of oxidative damage of brain tissue was measured after exposure. In addition, inhaled BALB/C mice were exposed to gaseous formaldehyde of 3.0 mg / m ~ (3) for 6 h 脳 7 d, and forebrain tissue was taken immediately after exposure. The expression of Grin2a Grin2b receptor subunit Grin2a Grin2b gene was detected by semi-quantitative RT-PCR method. The results showed that the escape latency of each group decreased with the increase of the experimental days, and the days of the experiment were significantly affected by different concentrations of formaldehyde inhaled FG 8.84 (P0.01), and the results showed that the escape latency of the mice decreased with the increase of the experimental days, and the days of the experiment were significantly affected by the different concentrations of formaldehyde inhaled FU 8.84 (P0.01). But there was no interaction between the two groups. The results showed that there was no significant difference between 0 mg / m3 formaldehyde inhalation group and 0.5 mg / m ~ (3) 1 mg / m ~ (3) formaldehyde inhalation group, but there was a very significant difference between 0 mg / m ~ (-3) formaldehyde inhalation group and 3.0 mg / m ~ (3) formaldehyde inhalation group (P0.01). In the space exploration experiment, the mice in the 0 mg / mt3 and 0.5 mg / mt3 formaldehyde inhaled groups had a longer swimming time in the quadrant where the platform was located. The swimming time in other quadrants was relatively short. 0 mg / m3 formaldehyde inhalation in the platform quadrant.) there was a significant difference between swimming time in SE quadrant and that in SE quadrant, and there was a significant difference between swimming time in other quadrants and swimming time in NWW quadrant. Compared with the other three quadrants, the swimming time of the formaldehyde inhalation group was significantly different from that of the other three quadrants, and the swimming time in the NE quadrant was significantly longer than that in the control group (P 0.01). The swimming time of mice in SE quadrant was the longest, but the swimming time in platform quadrant was not different from that in NE and SW quadrants. Compared with the control group, the content of malondialdehyde (MDAA) in mice exposed to 1.0 mg / mm3 and 3.0 mg / mm3 formaldehyde was significantly higher than that in the control group, and the GSH content and the activity of superoxide dismutase (SOD) in the control group were significantly lower than those in the control group. The changes of mRNA expression of NMDA receptor were as follows: compared with the control group, the expression of Grinl in formaldehyde inhaled group was significantly up-regulated, and the expression level of P0.05Agrin2b subunit was significantly increased. The expression of P0.01-Grin2a gene was significantly down-regulated in the formaldehyde inhaled group compared with the control group.
【學(xué)位授予單位】：華中師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R363

【引證文獻(xiàn)】

相關(guān)期刊論文 前3條

1 鄭健;;甲醛與人體健康[J];大學(xué)化學(xué);2009年01期

2 張藝濱;吳傳楠;楊慧敏;郝德;張林枝;王勝;顧饒勝;;甲醛毒性作用的研究進(jìn)展[J];吉林醫(yī)藥學(xué)院學(xué)報(bào);2008年04期

3 陳玉蘭;李賢新;陳建文;;甲醛的來源及毒性作用研究進(jìn)展[J];職業(yè)與健康;2010年21期

相關(guān)碩士學(xué)位論文 前2條

1 陳鑫;姜黃素和硒對甲醛毒性的拮抗作用研究[D];武漢科技大學(xué);2012年

2 李崇磊;揮發(fā)性有機(jī)物致小鼠學(xué)習(xí)記憶障礙及機(jī)理研究[D];大連理工大學(xué);2013年

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本文編號：1485923