本文關(guān)鍵詞： 流感病毒A型 HN亞型 疫苗 裂解工藝 透射電子顯微鏡　出處：《中國(guó)生物制品學(xué)雜志》2014年03期 　論文類型：期刊論文

【摘要】：目的透射電子顯微鏡觀察不同工藝制備的甲型H1N1流感病毒裂解疫苗形態(tài),篩選最佳裂解工藝,為疫苗的大規(guī)模生產(chǎn)提供參考依據(jù)。方法應(yīng)用透射電鏡對(duì)4種不同裂解工藝制備的甲型H1N1流感病毒疫苗進(jìn)行形態(tài)觀察,采用中和試驗(yàn)分析其免疫原性,異常毒性試驗(yàn)分析其安全性。結(jié)果透射電鏡觀察顯示4種樣品均無(wú)完整病毒,樣品1裂解程度最大,囊膜破裂,多數(shù)為病毒碎片;樣品2大多數(shù)囊膜未破損,缺少纖突;樣品3較完整的病毒顆粒略小(約30~40 nm),纖突清晰;樣品4大多數(shù)病毒顆粒保留著纖突,但纖突已不整齊清晰。樣品2組小鼠中和抗體滴度最低,而樣品1、3、4組較高,與樣品2組相比,差異明顯,其中以樣品3組最高;樣品2組小鼠、豚鼠體重增加不明顯,而樣品1、3、4組小鼠、豚鼠體重增加明顯,與樣品2組相比差異顯著,其中以樣品3組最明顯。結(jié)論電鏡觀察可直觀反映疫苗裂解程度;3種裂解工藝均可用于疫苗生產(chǎn),其中樣品3具有較好的免疫原性和安全性,其工藝可作為最佳裂解工藝用于后續(xù)疫苗生產(chǎn)。
[Abstract]:Objective to observe the morphology of influenza A (H1N1) virus lytic vaccine prepared by transmission electron microscope (TEM) and to screen the best lysis technique. Methods using transmission electron microscope (TEM) to observe the morphology of four kinds of influenza A (H1N1) vaccine prepared by different pyrolysis process, and to analyze its immunogenicity by neutralization test. Results the results of transmission electron microscopy showed that none of the four samples had intact virus, and sample 1 had the largest cleavage degree and the rupture of the capsule membrane, most of which were virus fragments. Most of the capsule in sample 2 were not damaged and lack of fibrous process. The virus particles in sample 3 were slightly smaller (about 30 ~ 40 nm) and the fibrils were clear. The titer of neutralizing antibody in group 2 was the lowest, while the titer of neutralizing antibody in group 1 was higher than that in group 2, which was significantly different from that in group 2. Among them, the sample group 3 was the highest. The weight gain of guinea pigs in group 2 was not significant, but that in group 1 was significantly higher than that in group 2. Conclusion Electron microscope observation can directly reflect the degree of vaccine cleavage. All of the three lytic processes can be used in vaccine production, among which sample 3 has better immunogenicity and safety, and its process can be used as the best lysis process for subsequent vaccine production.
【作者單位】： 中國(guó)人民解放軍軍事醫(yī)學(xué)科學(xué)院軍事獸醫(yī)研究所吉林省人獸共患病預(yù)防與控制重點(diǎn)實(shí)驗(yàn)室;長(zhǎng)春生物制品研究所有限責(zé)任公司;
【分類號(hào)】：R373.13
【正文快照】： 近年來(lái),市場(chǎng)上的流感疫苗均為純化的滅活疫苗,包括全病毒滅活疫苗、裂解疫苗及亞單位疫苗,其中裂解疫苗因其免疫效果好、副作用小而被廣泛應(yīng)用[1]。該疫苗是選用適當(dāng)裂解劑和裂解條件對(duì)流感病毒進(jìn)行裂解,經(jīng)純化除雜等步驟后制備,病毒裂解不完全或裂解過(guò)度均將直接影響疫苗質(zhì)量

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