本文關(guān)鍵詞： 氟中毒 腎臟 骨橋蛋白　出處：《吉林大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】： 腎臟作為氟的主要排泄器官,在氟中毒非骨相損害中越來越受到重視,有關(guān)氟中毒腎損害的文獻(xiàn)日漸增多,但過量氟引起腎損害的機(jī)制迄今尚未完全闡明。本文在大鼠動物模型與體外腎小管上皮細(xì)胞培養(yǎng)相結(jié)合的基礎(chǔ)上,應(yīng)用形態(tài)學(xué)、免疫組織化學(xué)、生物化學(xué)、分子生物學(xué)、觀察氟中毒腎損害的性質(zhì)、特點著重探討骨橋蛋白在腎損害發(fā)生發(fā)展中的作用。本文的主要結(jié)果和結(jié)論是:①不同鈣營養(yǎng)狀況、不同投氟時間和投氟劑量引起的腎臟病理改變不盡相同應(yīng)用圖象分析儀對腎小囊囊腔、腎小管管腔的面積及直徑參數(shù)進(jìn)行定量分析,發(fā)現(xiàn)在常食和偏食染氟組腎小囊腔均較對照組擴(kuò)張明顯;對皮髓質(zhì)交界處的腎小管腔的等圓直徑進(jìn)行測量,染氟同等劑量和同樣時間的情況下,偏食組較常食組擴(kuò)張明顯。②氟中毒腎損害的發(fā)生發(fā)展中有氧化應(yīng)激參與,但氧化應(yīng)激不象是始動環(huán)節(jié)或唯一機(jī)制。③氟中毒腎組織OPN的表達(dá)增強(qiáng)可能具有腎保護(hù)作用;OPN可在血清和尿中檢出,故可能成為氟中毒腎損害的一種標(biāo)志物。
[Abstract]:As the main excretory organ of fluoride, kidney is paid more and more attention in the fluorosis non-bone damage, and the literature about fluorosis kidney damage is increasing day by day. However, the mechanism of renal damage caused by excessive fluoride has not been fully elucidated. Based on the combination of rat model and renal tubular epithelial cell culture in vitro, morphology, immunohistochemistry and biochemistry were used. Molecular biology was used to observe the nature of renal damage caused by fluorosis. The role of osteopontin in the development of renal damage was discussed. The main results and conclusions were as follows: 1. The pathological changes of kidney caused by different fluorine time and dose were analyzed quantitatively by image analyzer in the area and diameter of renal microsac and tubule lumen. It was found that the dilatation of renal microsac was more obvious in the fluorine-exposed group than in the control group. The isodyclic diameters of the renal tubule cavity at the junction of the dermatomedullary were measured at the same dose and at the same time. Oxidative stress was involved in the occurrence and development of renal damage caused by fluorosis in the partial diet group compared with the normal diet group. 2. However, oxidative stress does not appear to be the initiator or the only mechanism. 3. The increased expression of OPN in renal tissue of fluorosis may have renal protective effect. OPN can be detected in serum and urine, so it may be a marker of renal damage caused by fluorosis.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R363

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