本文關(guān)鍵詞： 苛求芽孢桿菌尿酸酶 血清尿酸測定專利尿酸酶法 黃嘌呤干擾 簡并引物PCR 基因克隆　出處：《重慶醫(yī)科大學》2006年碩士論文　論文類型：學位論文

【摘要】： 測定血清尿酸是臨床檢驗常規(guī)工作之一。利用尿酸酶的高專一性進行酶法分析測定血清尿酸含量,即尿酸酶法,是目前常規(guī)測定血清尿酸的主要方法�，F(xiàn)有尿酸酶法中,過氧化物酶偶聯(lián)尿酸酶的間接終點平衡法最常用,但此終點平衡法受血清中還原物質(zhì)和過氧化物,及黃嘌呤等的顯著干擾。本實驗室在國家自然科學基金(30200266)資助下,建立了血清尿酸含量測定的專利方法(zl 03135649.4),此專利方法對血清中常見干擾物質(zhì)不敏感;與其它尿酸酶法相比,相同效率下其成本最低,線性范圍最寬,對黃嘌呤抗性最強。已報道尿酸酶法中,測定尿酸酶作用下紫外吸收總變化的直接終點平衡法對常見干擾抗性最強。本實驗室的專利方法與直接終點平衡法一致,但與最常用的過氧化物酶偶聯(lián)尿酸酶間接終點平衡法不一致�？梢姶藢＠椒ǹ尚星铱煽俊５藢＠椒ㄐ枰狵_m較高且對黃嘌呤抑制不敏感的工具尿酸酶。目前市售尿酸酶K_m都較低且對黃嘌呤敏感。另一方面,尿酸酶也是當前治療高尿酸血癥相關(guān)疾病的理想藥物�？燎笱挎邨U菌依賴于高濃度尿酸而生長,其可表達高比活性的胞內(nèi)和胞外尿酸酶;此類尿酸酶K_m較高,最接近我們專利方法要求;但至今未見關(guān)于此物種的任何基因序列信息報道。為獲得廉價且符合要求尿酸酶以促進測定血清
[Abstract]:Determination of serum uric acid is one of the routine tasks of clinical examination. The indirect end point equilibrium method of peroxidase conjugated uricase is the most commonly used, but this end point equilibrium method is by reducing substance and peroxide in serum. And the significant interference of xanthine. The patent method for the determination of serum uric acid was established in our laboratory with the aid of the National Natural Science Foundation of China (30200266). The patent method for determination of serum uric acid is zl03135649.4). The patented method is insensitive to common interfering substances in serum. Compared with other uric acidase methods, it has the lowest cost, the widest linear range and the strongest resistance to xanthine at the same efficiency. The direct end-point equilibrium method for the determination of total ultraviolet absorption under the action of uric acid enzyme is the most resistant to common interference. The patent method in our laboratory is consistent with the direct end-point equilibrium method. However, it is not consistent with the most commonly used indirect end-point equilibrium method for peroxidase conjugated uric acidase. It can be seen that the patented method is feasible and reliable. However, the patented method requires uric acid enzyme with higher km and less sensitivity to xanthine inhibition. At present, uric acidase K _ m is low and sensitive to xanthine, on the other hand. Urease is also an ideal drug for the treatment of hyperuricemia related diseases. Bacillus causticus is dependent on high concentration of uric acid and can express high specific activity of intracellular and extracellular uric acidase. This kind of uric acid enzyme K _ S _ m is relatively high and closest to our patented method requirements; However, no information on the gene sequence of the species has been reported. To obtain cheap and satisfactory uric acid enzymes to facilitate the determination of serum
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R378

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