本文關(guān)鍵詞：免疫組化穩(wěn)定表達(dá)與精細(xì)操作　出處：《診斷病理學(xué)雜志》2014年10期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： 免疫組化 穩(wěn)定表達(dá) 精細(xì)操作

【摘要】：正免疫組化染色是一項(xiàng)綜合性的操作技術(shù),其影響因素復(fù)雜,加強(qiáng)質(zhì)量管理和提高可信性尤為重要。我科從2001年開(kāi)展免疫組化染色,在具體的操作過(guò)程中進(jìn)行了精細(xì)而有效的全程質(zhì)控,現(xiàn)將質(zhì)控事項(xiàng)綜述如下。1標(biāo)本固定固定液為4%中性緩沖甲醛(10 ml甲醛+90 ml PBS緩沖液)。要求離體后及時(shí)固定,固定液的量為標(biāo)本體積的5~10倍。固定不當(dāng),固定時(shí)間不夠或過(guò)長(zhǎng),都可引起抗原丟失,尤其是一些較脆弱的膜抗原(如CD20等),故固定應(yīng)及時(shí)并充
[Abstract]:Positive immunohistochemical staining is a comprehensive operation technique, its influence factors are complex, it is very important to strengthen quality management and improve credibility. Fine and effective quality control is carried out in the concrete operation process. In this paper, the quality control items are summarized as follows. 1 the fixed solution is 4% neutral buffer formaldehyde 10 ml formaldehyde 90 ml PBS buffer. The amount of fixed solution is 5 ~ 10 times of the volume of specimen. Improper fixation, insufficient or too long fixation time can cause antigen loss, especially some more vulnerable membrane antigens (such as CD20, etc.), so the fixation should be timely and charged.
【作者單位】： 龍巖第二醫(yī)院病理科;
【分類(lèi)號(hào)】：R361.2
【正文快照】： 免疫組化染色是一項(xiàng)綜合性的操作技術(shù),其影響因素復(fù)雜,加強(qiáng)質(zhì)量管理和提高可信性尤為重要。我科從2001年開(kāi)展免疫組化染色,在具體的操作過(guò)程中進(jìn)行了精細(xì)而有效的全程質(zhì)控,現(xiàn)將質(zhì)控事項(xiàng)綜述如下。1標(biāo)本固定固定液為4%中性緩沖甲醛(10 ml甲醛+90 ml PBS緩沖液)。要求離體后及時(shí)，

本文編號(hào)：1438095