本文關(guān)鍵詞：結(jié)核分枝桿菌L型的誘導(dǎo)及其對(duì)抗菌藥物的敏感性　出處：《山東醫(yī)藥》2014年39期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： 分支桿菌 結(jié)核 細(xì)菌L型 抗結(jié)核藥物

【摘要】：目的觀察異煙肼、利福平、乙胺丁醇抗結(jié)核藥物誘導(dǎo)形成結(jié)核分支桿菌L型以及結(jié)核分支桿菌L型對(duì)抗菌藥物敏感性。方法將結(jié)核分支桿菌接種于含有不同質(zhì)量濃度利福平(0.05、0.1、0.2μg/m L)、異煙肼(0.01、0.04、0.08μg/m L)、乙胺丁醇(1、2.5、5μg/m L)的BD960液體培養(yǎng)基內(nèi),10 d后在顯微鏡下觀察結(jié)核分支桿菌L型的形成情況。培養(yǎng)結(jié)核分支桿菌L型,在顯微鏡高倍鏡下觀察,并檢測(cè)其對(duì)抗菌藥物的敏感性。結(jié)果 0.1、0.2μg/m L利福平,0.04、0.08μg/m L異煙肼,2.5、5μg/m L乙胺丁醇可誘導(dǎo)形成結(jié)核分支桿菌L型。在含有4μg/m L鏈霉素、0.2μg/m L異煙肼、40μg/m L利福平、2μg/m L乙胺丁醇、1μg/m L對(duì)氨基水楊酸的非高滲培養(yǎng)基內(nèi)培養(yǎng)7 d后均可見(jiàn)結(jié)核分枝桿菌L型生長(zhǎng),而含有30μg/m L卡那霉素、40μg/m L卷曲霉素、3μg/m L氧氟沙星的培養(yǎng)基內(nèi)未見(jiàn)其生長(zhǎng)。結(jié)論中高濃度的利福平、異煙肼、乙胺丁醇能夠誘導(dǎo)結(jié)核分支桿菌L型形成。結(jié)核分枝桿菌L型對(duì)卡那霉素、卷曲霉素、氧氟沙星敏感。
[Abstract]:Objective to observe isoniazid and rifampicin. Methods Mycobacterium tuberculosis was induced to form mycobacterium tuberculosis L form and Mycobacterium tuberculosis L form was sensitive to antimicrobial agents. Methods Mycobacterium tuberculosis was inoculated with rifampicin of different concentrations. 0.1U 0.2 渭 g / m L ~ (-1), 0.011 渭 g / m ~ (-1) of isoniazid, 0.08 渭 g / m ~ (-1) L ~ (-1) of ethambutanol, 2.5 渭 g / m ~ (-1) of ethambutanol. The formation of mycobacterium tuberculosis L-form was observed under microscope after 10 days in 5 渭 g / mL BD960 liquid medium. The L-form of mycobacterium tuberculosis was cultured and observed under high power microscope. The sensitivity to antimicrobial agents was determined. Results 0.1 渭 g / mL rifampicin 0.08 渭 g / mL isoniazid 2.5 was used. The L-form of Mycobacterium tuberculosis was induced by 5 渭 g / mL ethambutanol and 40 渭 g / mL rifampicin containing 4 渭 g / mL streptomycin 0.2 渭 g / mL isoniazid. 2 渭 g / mL ethambutanol 1 渭 g / mL in non-hypertonic culture medium of aminosalicylic acid for 7 days all showed L-form growth of Mycobacterium tuberculosis, but contained 30 渭 g / m L kanamycin. There was no growth of ofloxacin in 40 渭 g / mL ofloxacin 3 渭 g / mL ofloxacin. Conclusion Rifampicin and isoniazid at medium and high concentrations were not found. The L-form of Mycobacterium tuberculosis is sensitive to kanamycin, capramycin and ofloxacin.
【作者單位】： 天津市海河醫(yī)院;
【基金】：天津市衛(wèi)生局科技基金項(xiàng)目(2013KY13)
【分類(lèi)號(hào)】：R378.911
【正文快照】： 結(jié)核病嚴(yán)重影響人類(lèi)健康,抗結(jié)核藥物的出現(xiàn)使結(jié)核病得到有效控制,但近10年,結(jié)核病疫情迅速回升,尤其耐藥結(jié)核病的流行,為結(jié)核病疫情的控制帶來(lái)更大的挑戰(zhàn)。有研究[1,2]表明,結(jié)核分枝桿菌菌壁缺失變異形成L型后可長(zhǎng)期潛伏于宿主體內(nèi)導(dǎo)致結(jié)核病復(fù)燃。同時(shí)有研究[3]表明,結(jié)核分枝

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