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  本文關鍵詞：結核分枝桿菌L型的誘導及其對抗菌藥物的敏感性　出處：《山東醫(yī)藥》2014年39期 　論文類型：期刊論文

  更多相關文章： 分支桿菌 結核 細菌L型 抗結核藥物

【摘要】：目的觀察異煙肼、利福平、乙胺丁醇抗結核藥物誘導形成結核分支桿菌L型以及結核分支桿菌L型對抗菌藥物敏感性。方法將結核分支桿菌接種于含有不同質(zhì)量濃度利福平(0.05、0.1、0.2μg/m L)、異煙肼(0.01、0.04、0.08μg/m L)、乙胺丁醇(1、2.5、5μg/m L)的BD960液體培養(yǎng)基內(nèi),10 d后在顯微鏡下觀察結核分支桿菌L型的形成情況。培養(yǎng)結核分支桿菌L型,在顯微鏡高倍鏡下觀察,并檢測其對抗菌藥物的敏感性。結果 0.1、0.2μg/m L利福平,0.04、0.08μg/m L異煙肼,2.5、5μg/m L乙胺丁醇可誘導形成結核分支桿菌L型。在含有4μg/m L鏈霉素、0.2μg/m L異煙肼、40μg/m L利福平、2μg/m L乙胺丁醇、1μg/m L對氨基水楊酸的非高滲培養(yǎng)基內(nèi)培養(yǎng)7 d后均可見結核分枝桿菌L型生長,而含有30μg/m L卡那霉素、40μg/m L卷曲霉素、3μg/m L氧氟沙星的培養(yǎng)基內(nèi)未見其生長。結論中高濃度的利福平、異煙肼、乙胺丁醇能夠誘導結核分支桿菌L型形成。結核分枝桿菌L型對卡那霉素、卷曲霉素、氧氟沙星敏感。
[Abstract]:Objective to observe isoniazid and rifampicin. Methods Mycobacterium tuberculosis was induced to form mycobacterium tuberculosis L form and Mycobacterium tuberculosis L form was sensitive to antimicrobial agents. Methods Mycobacterium tuberculosis was inoculated with rifampicin of different concentrations. 0.1U 0.2 渭 g / m L ~ (-1), 0.011 渭 g / m ~ (-1) of isoniazid, 0.08 渭 g / m ~ (-1) L ~ (-1) of ethambutanol, 2.5 渭 g / m ~ (-1) of ethambutanol. The formation of mycobacterium tuberculosis L-form was observed under microscope after 10 days in 5 渭 g / mL BD960 liquid medium. The L-form of mycobacterium tuberculosis was cultured and observed under high power microscope. The sensitivity to antimicrobial agents was determined. Results 0.1 渭 g / mL rifampicin 0.08 渭 g / mL isoniazid 2.5 was used. The L-form of Mycobacterium tuberculosis was induced by 5 渭 g / mL ethambutanol and 40 渭 g / mL rifampicin containing 4 渭 g / mL streptomycin 0.2 渭 g / mL isoniazid. 2 渭 g / mL ethambutanol 1 渭 g / mL in non-hypertonic culture medium of aminosalicylic acid for 7 days all showed L-form growth of Mycobacterium tuberculosis, but contained 30 渭 g / m L kanamycin. There was no growth of ofloxacin in 40 渭 g / mL ofloxacin 3 渭 g / mL ofloxacin. Conclusion Rifampicin and isoniazid at medium and high concentrations were not found. The L-form of Mycobacterium tuberculosis is sensitive to kanamycin, capramycin and ofloxacin.
【作者單位】： 天津市海河醫(yī)院;
【基金】：天津市衛(wèi)生局科技基金項目(2013KY13)
【分類號】：R378.911
【正文快照】： 結核病嚴重影響人類健康,抗結核藥物的出現(xiàn)使結核病得到有效控制,但近10年,結核病疫情迅速回升,尤其耐藥結核病的流行,為結核病疫情的控制帶來更大的挑戰(zhàn)。有研究[1,2]表明,結核分枝桿菌菌壁缺失變異形成L型后可長期潛伏于宿主體內(nèi)導致結核病復燃。同時有研究[3]表明,結核分枝

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