本文關(guān)鍵詞：不同地理區(qū)域異盤并殖吸蟲形態(tài)學(xué)研究　出處：《昆明醫(yī)學(xué)院》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 異盤并殖吸蟲 掃描電鏡 超微結(jié)構(gòu) 馬關(guān) 越南

【摘要】：[目的] 調(diào)查云南馬關(guān)異盤并殖吸蟲的流行情況;觀察馬關(guān)異盤并殖吸蟲囊蚴、成蟲和蟲卵的形態(tài):對(duì)中國(guó)云南和越南的異盤并殖吸蟲成蟲體表進(jìn)行掃描電鏡觀察。補(bǔ)充異盤并殖吸蟲的形態(tài)學(xué)資料,研究不同地理株的形態(tài)差異,探討成蟲皮棘的形態(tài)和排列規(guī)律及其在蟲種鑒定中的意義。 [方法]從馬關(guān)采集溪蟹,分離囊蚴,形態(tài)觀測(cè)鑒定后,經(jīng)口飼感染家貓,收集成蟲和蟲卵標(biāo)本進(jìn)行形態(tài)學(xué)觀察和測(cè)量,并在高真空和低真空環(huán)境下對(duì)成蟲腹面的體表進(jìn)行掃描電鏡觀察。 [結(jié)果]云南馬關(guān)異盤并殖吸蟲的活體囊蚴橢圓形,具兩層囊壁,大小為266.97±22.25×248.98±22.51μm,外壁薄,內(nèi)壁厚并向兩極明顯增厚,內(nèi)壁側(cè)厚3.94±2.02μm,內(nèi)壁端厚13.35±2.97μm;成蟲大小12.6±1.51×7.1±1.01mm,寬長(zhǎng)比平均為1:1.78;口吸盤顯著大于腹吸盤,口、腹吸盤之比為1.89:1。第二中間宿主—溪蟹的自然感染率是75%。人工感染家貓的成蟲回收率為60%。 在高真空環(huán)境下,馬關(guān)的異盤并殖吸蟲成蟲皮棘為單生型、呈指狀。在低真空環(huán)境下,馬關(guān)成蟲皮棘單生、半環(huán)狀,與體壁凹陷共呈蝌蚪狀;越南成蟲有5種單生皮棘:三角形或半圓形、鯊魚嘴狀、山丘狀、魚鱗狀和半環(huán)形。口、腹吸盤周圍均未見乳突。 [結(jié)論]云南馬關(guān)是異盤并殖吸蟲流行區(qū),屬高度疫源地。囊蚴橢圓形,具兩層囊壁,大小為266.97±22.25×248.98±22.51μm,外壁薄,內(nèi)壁厚并向兩極明顯增厚。成蟲皮棘屬單生型,不同地理株之間存在一定差異。家貓是其適宜實(shí)驗(yàn)終宿主。
[Abstract]:[Objective] to investigate the prevalence of paragonimiasis in Maguan, Yunnan. To observe the morphology of metacercariae, adults and eggs of paragonimus Maguan: scanning electron microscope was used to observe the surface of adults of paragonimiasis in Yunnan and Vietnam, China, and to supplement the morphological data of paragonimiasis. To study the morphological differences of different geographic strains and to explore the morphology and arrangement of adult bark spine and its significance in the identification of insect species. [Methods] the crab was collected from Maguan and the cysticercaria was isolated. After morphological observation and identification, the infected cats were fed orally and the adult and egg specimens were collected for morphological observation and measurement. The abdominal surface of adult was observed by scanning electron microscope in high vacuum and low vacuum environment. [Results] the living cysticercariae of paragonimiasis Maguan in Yunnan Province were oval with two layers of cyst wall, the size was 266.97 鹵22.25 脳 248.98 鹵22.51 渭 m, and the outer wall was thin. The thickness of the inner wall was 3.94 鹵2.02 渭 m and that of the end of the inner wall was 13.35 鹵2.97 渭 m. The adult's size was 12.6 鹵1.51 脳 7.1 鹵1.01mm, and the average aspect ratio was 1: 1.78; The ratio of oral sucker to abdominal sucker was 1.89: 1.The natural infection rate of the second intermediate host was 75. The adult recovery rate of artificial infected cats was 60. Under the high vacuum environment, the skin spines of adult paragonimiasis of Maguan are monophytic and finger like. In the low vacuum environment, the skin spines of the adults of Maguan are monotrophic, semi-annular, and tadpole with the body wall depression. There are 5 species of monodermis spine in Vietnam: triangular or semicircular, shark beak, hilly, fish scale and semicircle. There is no mastoid around the ventral sucker. [Conclusion: Maguan, Yunnan Province, is an endemic area of paragonimiasis and belongs to a high foci. The cysticercaria is oval with two layers of cyst wall, and the size is 266.97 鹵22.25 脳 248.98 鹵22.51 渭 m. The outer wall is thin and the inner wall is thicker and thicker to the two poles. The adult dermatophytes belong to monophytic type, and there are some differences among different geographical strains. The domestic cat is the suitable host for the end of the experiment.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R383

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