本文關(guān)鍵詞：大鼠濕困脾胃動物模型復(fù)制的實驗研究　出處：《成都中醫(yī)藥大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 濕困脾胃 動物模型 宏觀體征 胃腸功能 胃腸激素 物質(zhì)能量 水液代謝 免疫功能 實驗研究 大鼠 平胃散

【摘要】：實驗?zāi)康模航⒋笫鬂窭⑽缸C動物模型及其生物學(xué)特征。 實驗方法：采用增加濕度、飲食調(diào)控結(jié)合睡眠控制的方法建立大鼠濕困脾胃證動物模型，選擇經(jīng)典方藥平胃散作為反證治療藥物；用定量、半定量方法觀察大鼠濕困脾胃證動物模型的宏觀體征；用半固體營養(yǎng)糊法測定大鼠的胃和小腸運(yùn)動功能變化；用離子選擇電極法測定血清電解質(zhì)；用GOD-PAP法測定血糖(Glu)；用雙縮脲法測定總蛋白(TP)；用GPO-PAP法測定甘油三脂(TG)；用CHOD-PAP法測定總膽固醇(TC)；用ELISA法測定血清胃泌素(Gas)、免疫球蛋白-G(IgG)、胰島素(Insulin)、白細(xì)胞介素-6(IL-6)；用放免法檢測血清胃動素(MTL)；用比色法測定肝細(xì)胞Na~+-K~+-ATP酶活性；用HE染色法觀察脾、胸腺及胃腸組織形態(tài)學(xué)變化。 實驗結(jié)果：1 大鼠濕困脾胃證動物模型及其生物學(xué)特征的建立①宏觀體征：模型形成時間較長；難以速愈，模型體征短期內(nèi)不易恢復(fù)；模型大鼠生長狀態(tài)不佳，皮毛濕濁色澤晦暗發(fā)黃，消瘦，體重減輕，體長、尾長、尾根直徑增長緩慢；食欲不振，飲食量減少；小便黃量少，大便溏濕重增加；腹脹，腹圍指數(shù)增加；精神萎靡，陰囊松弛下垂，嗜臥懶動，自發(fā)活動次數(shù)減少；口粘膩，口腔PH值微升；體溫微升或不變；與空白對照組比較有改變趨勢或有顯著性差異(P＜0.05或P＜0.01)。②胃腸功能：模型動物胃腸脹氣明顯，胃排空和小腸推動能力下降，胃腸吸收功能異常，胃腸激素水平紊亂，胃腸組織發(fā)生病理改變，與空白對照組比較有改變趨勢或有顯著性差異(P＜0.05或P＜0.01)。③物質(zhì)能量代謝：模型大鼠臟器系數(shù)增加，血清電解質(zhì)水平紊亂，，糖、蛋白、脂類含量異常，胰島素水平下降，Na~+-K~+-ATP酶活性降低，與空白對照組比較有改變趨勢或有顯著性差異(P＜0.05或P＜0.01)。④免疫功能：模型脾臟及胸腺臟器系數(shù)降低，胸腺皮質(zhì)厚度及脾臟中央動脈淋巴鞘直徑、面積變小，血清IL-6升高，IgG含量明顯減少，與空白對照組比較有改變趨勢或有顯著性差異(P＜0.05或P＜0.01)。2 反證藥物平胃散對該模型的治療作用：經(jīng)平胃散治療后，治療組大鼠生長良好，皮毛濃密而有光澤，在宏觀體征、胃腸功能、物質(zhì)能量代謝、免疫功能四方面的多數(shù)指標(biāo)均有所改善或恢復(fù)正常，與模型組、自然恢復(fù)組比較有改變趨勢或有顯著性差異(P＜0.05或P＜0.01)。 實驗結(jié)論：本實驗制備的大鼠濕困脾胃證動物模型，從宏觀體征、胃腸功能、物質(zhì)能量代謝、免疫功能四個方面具有顯著的生物學(xué)特征，經(jīng)典方藥平胃散對該模型良好的治療作用也說明了模型的成功性。因此我們認(rèn)為采用增加濕度、飲食調(diào)控結(jié)合睡眠控制的方法制備的大鼠濕困脾胃證動物模型是一個比較符合中醫(yī)理論的動物模型。
[Abstract]:Objective: to establish an animal model of dampness of spleen and stomach and its biological characteristics. Methods: the animal model of spleen and stomach dampness was established by increasing humidity, diet regulation and sleep control. The macroscopical signs of rat model of dampness in spleen and stomach were observed by quantitative and semi-quantitative methods. The changes of gastric and intestinal motor function in rats were measured by semisolid nutritional paste method. Serum electrolytes were determined by ion selective electrode method. Blood glucose was determined by GOD-PAP method. The total protein TPX was determined by biuret method. GPO-PAP method was used for the determination of triglyceride. Total cholesterol (TC) was determined by CHOD-PAP method. The levels of serum gastrin, immunoglobulin G, insulin, interleukin 6 and interleukin 6 were determined by ELISA method. The serum motilin motilin (MTL) was detected by radioimmunoassay. The activity of Na ~ -K ~ -ATPase in hepatocytes was measured by colorimetry. The morphological changes of spleen, thymus and gastrointestinal tissues were observed by HE staining. Results the animal model of dampness of spleen and stomach and its biological characteristics were established. 1 macroscopic signs: the formation time of the model was longer; It is difficult to recover quickly and the physical signs of the model are not easy to recover in a short time. The growth state of the model rats was not good, the fur was dark and yellow, thin, weight loss, body length, tail length, tail root diameter growth slow; Loss of appetite and reduced diet; Urination Huang Liang little, loose stools wet weight increase; Abdominal distension, abdominal circumference index increased; The spirit is dismal, scrotum flabby and drooping, lying lazy and moving, and the number of spontaneous activities decreases; The mouth is sticky and greasy, and the PH value of the mouth increases slightly; Slight rise or no change in body temperature; Compared with the blank control group, there were significant differences in gastrointestinal function (P < 0.05 or P < 0.01). Abnormal gastrointestinal absorption function, gastrointestinal hormone level disorder, gastrointestinal tissue pathological changes. Compared with the blank control group, there were significant differences in substance and energy metabolism (P < 0.05 or P < 0.01). The lipid content was abnormal, the insulin level was decreased and the Na ~ -K ~ -ATPase activity was decreased. Compared with the blank control group, the immune function of the model was significantly different (P < 0.05 or P < 0.01). The spleen and thymus organ coefficients of the model were lower than that of the blank control group (P < 0.05 or P < 0.01). The thickness of thymic cortex and the diameter and area of lymphatic sheath of the central splenic artery became smaller, and the content of serum IL-6 increased significantly. Compared with the blank control group, there were significant differences (P < 0.05 or P < 0.01). 2 the therapeutic effect of Pingwei Powder on the model: after treatment with Pingwei Powder. The treatment group rats grew well, fur thick and shiny, in the macro signs, gastrointestinal function, substance and energy metabolism, immune function of the four indicators are improved or returned to normal, with the model group. In natural recovery group, there was a change trend or significant difference (P < 0.05 or P < 0.01). Conclusion: the animal model of dampness of spleen and stomach in this experiment has significant biological characteristics in four aspects: macroscopic signs, gastrointestinal function, substance and energy metabolism, and immune function. The good therapeutic effect of classical prescription Pingwei Powder on the model also shows the success of the model. The animal model of dampness of spleen and stomach was made by the method of diet regulation and sleep control.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R-332

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 劉瑤;邱蔚芬;;平胃散實驗研究進(jìn)展[J];中國藥物濫用防治雜志;2012年04期

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