發(fā)布時(shí)間：2018-01-15 12:20

  本文關(guān)鍵詞：大鼠重度閉合性顱腦損傷模型構(gòu)建及傷后S100β蛋白表達(dá)與腦損傷時(shí)間關(guān)系的研究　出處：《延邊大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 彌漫性軸索損傷 動(dòng)物模型 S100β 損傷時(shí)間 免疫組織化學(xué)

【摘要】： 目的：改進(jìn)Marmarou法制作大鼠重度彌漫性腦損傷模型，觀察大鼠重度彌漫性腦損傷后S100β蛋白在腦組織中不同部位的時(shí)間相關(guān)性表達(dá)，并以此為彌漫性腦損傷時(shí)間推斷、生前傷和死后傷判斷及早期診斷提供實(shí)驗(yàn)依據(jù)。 方法：使用改進(jìn)后的Marmarou致傷裝置，，以沖擊力(180cm×450g)打擊大鼠頭部制作重度彌漫性顱腦損傷動(dòng)物模型，觀察損傷后動(dòng)物神經(jīng)系統(tǒng)體征及腦組織病理形態(tài)學(xué)變化。應(yīng)用免疫組織化學(xué)方法和圖像分析技術(shù)，檢測彌漫性腦損傷后30min，2h，4h，12h，24h，3d，7d，死后10min后損傷，各組S100β蛋白在大腦皮質(zhì)、海馬、丘腦和腦干中神經(jīng)膠質(zhì)細(xì)胞的表達(dá)，并設(shè)立正常組作對(duì)照。 結(jié)果：(1)與對(duì)照組相比，損傷后大鼠神經(jīng)系統(tǒng)體征有明顯改變，病理學(xué)檢查各致傷組動(dòng)物均有彌漫性腦損傷。(2)與對(duì)照組相比，S100β陽性細(xì)胞個(gè)數(shù)及蛋白表達(dá)在皮質(zhì)、海馬和丘腦形成先增后降再升的曲線，即兩次表達(dá)高峰，腦干表達(dá)變化不顯著；在傷后2h，海馬、丘腦S100β表達(dá)細(xì)胞個(gè)數(shù)和蛋白量開始增加，4h有顯著增加，12h達(dá)到高峰值，皮質(zhì)部位在4h達(dá)到高峰。傷后7天恢復(fù)正常值。 結(jié)論：(1)改進(jìn)后的大鼠彌漫性腦損傷模型穩(wěn)定、實(shí)用、重復(fù)性好；打擊傷組動(dòng)物動(dòng)物死亡率低且病理變化典型，適合動(dòng)態(tài)研究彌漫性腦損傷后的病理生理變化。(2)彌漫性腦損傷后S100β在不同部位表達(dá)有不同規(guī)律性且有較好的時(shí)間相關(guān)性，可成為法醫(yī)腦損傷時(shí)間推斷的一種有效指標(biāo)。
[Abstract]:Objective: to establish the rat model of diffuse brain injury with modified Marmarou method, were observed in rats with severe diffuse brain injury after S100 beta protein in the brain tissue of time correlation in different parts of the expression, and as a diffuse brain injury time estimation, antemortem and after injury and provide experimental basis for early stage diagnosis of judgment.
Methods: using the improved Marmarou injury device, the impact force (180cm * 450g) on the rat head produced severe diffuse brain injury animal model, the pathological changes of brain tissue and animal signs of nervous system injury were observed. Immunohistochemical method and image analysis technology, 30min detection of diffuse brain injury. After 2h, 4h, 12h, 24h, 3D, 7d, 10min after death after injury, the S100 protein in the cerebral cortex, hippocampus, thalamus and the expression of glial cells in the brain stem, and the establishment of normal group as control.
Results: (1) compared with the control group, after the injury of the rat nervous system signs changed obviously, pathological examination of each animal injury group had diffuse brain injury. (2) compared with the control group, the expression of S100 beta positive cell number and protein in cortex, hippocampus and thalamus form falling or curve first after the increase, which is two times the peak expression did not change significantly, the expression of brain stem; hippocampus at 2h after injury, the expression of S100 in thalamus, the cell number and protein content began to increase, 4H increased significantly, 12h reached the peak, the cortex peaked at 4H. 7 days after injury to restore normal values.
Conclusion: (1) improved after diffuse brain injury model in rats is stable, practical, good repeatability; combat injury group of animal mortality rate is low and the typical pathological changes, suitable for the dynamic study of the pathophysiological changes after diffuse brain injury. (2) S100 after diffuse brain injury in different parts have different expression of beta the regularity and the time correlation, can become an effective index of time forensic brain injury inference.

【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R-332;R651.15

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 賀曉生,章翔,易聲禹;彌漫性軸索損傷[J];中華神經(jīng)外科雜志;1999年01期

本文編號(hào)：1428345