本文關(guān)鍵詞：小鼠新分子mPEBP4的克隆、鑒定及其功能研究　出處：《浙江大學》2006年碩士論文　論文類型：學位論文

  更多相關(guān)文章： mPEBP4 克隆 乳腺癌 遷移 侵襲 凋亡 信號轉(zhuǎn)導

【摘要】：磷脂酰乙醇胺結(jié)合蛋白(Phosphatidylethanolamine-binding protein,PEBP)家族是一個分子量在21-23kDa之間的堿性蛋白家族。最早是因為能與細胞膜成份磷脂酰乙醇胺(Phosphatidylethanolamine,PE)結(jié)合而在牛腦中被發(fā)現(xiàn)。PEBP是一類進化上非常保守的蛋白家族,廣泛存在于植物、瘧原蟲、線蟲、果蠅及哺乳動物(包括牛、猴、人等)中。PEBP家族成員具有多種生物學功能,如結(jié)合脂質(zhì)、抑制絲氨酸蛋白酶活性等。有些PEBP成員還能作為十一氨基酸多肽海馬趾類膽堿神經(jīng)刺激肽(HCNP)前體蛋白,在海馬的發(fā)育中起著重要的作用。Raf-1和MEK都能與PEBP中高度保守的區(qū)域結(jié)合,而hPEBP1(又稱為Raf激酶抑制蛋白,RKIP,Raf kinase inhibitory protein)能抑制Raf-1激酶和絲裂原激活的蛋白激酶的活性,使MEK和Raf-1復合物解離,從而抑制了MEK的活化。最近有研究還表明PEBP的一家族成員RKIP的表達還能抑制前列腺癌的轉(zhuǎn)移。在我們以往從人骨髓基質(zhì)細胞(BMSC)cDNA文庫中自主發(fā)現(xiàn)的一種新型PEBP家族成員即人磷脂酰乙醇胺結(jié)合蛋白4(human phosphatidylethanolamine(PE)-bindign protein 4,hPEBP4)并證明其在乳腺癌高表達和發(fā)揮抗凋亡作用的基礎(chǔ)上,本研究擬克隆hPEBP4的小鼠同源分子mPEBP4,并對其生物學功能進行初步研究。 第一部分、mPEBP4的克隆和序列分析、組織細胞分布和細胞定位研究 如上所述,PEBP是一類能與磷脂酰乙醇胺(PE)結(jié)合的蛋白,該家族的所有成員都含有與PE結(jié)合的保守區(qū)域(PBP)。人磷脂酰乙醇胺結(jié)合蛋白4(human phosphatidylethanolamine(PE)-binding protein 4,hPEBP4)是我們從人骨髓基質(zhì)細胞(BMSC)cDNA文庫中自主發(fā)現(xiàn)的一種新型PEBP家族成員,其全長cDNA為874bp,編碼227氨基酸的蛋白。我們以往的研究表明該蛋白在核酸和氨基酸水
[Abstract]:Phosphatidylethanolamine binding protein (Phosphatidylethanolamine-binding protein PEBP) is a family of molecular weight between 21-23kDa alkaline protein family. The earliest is due to cell membrane component and phosphatidylethanolamine (Phosphatidylethanolamine, PE) and was found in the bovine brain.PEBP is an evolutionarily conserved protein family. Widely exists in plants, Plasmodium, nematode, Drosophila and mammals (including cows, monkeys, etc.) in the.PEBP family members with a variety of biological functions, such as lipid binding, inhibition of serine protease activity. Some members of PEBP can be used as a eleven amino acid polypeptide of hippocampal cholinergic neurostimulating peptide (HCNP) precursor protein in the hippocampus. The development plays a role of.Raf-1 and MEK are important conserved region and PEBP binding, and hPEBP1 (also called Raf kinase inhibitor protein, RKIP Raf, kinase inhibitor Y protein) can inhibit Raf-1 kinase and mitogen activated protein kinase activity, the MEK and Raf-1 complex dissociation, thereby inhibiting the activation of MEK. Recent studies also showed that the expression of PEBP can transfer a member of the family of RKIP inhibits prostate cancer. In our previous from human bone marrow stromal cells (BMSC) a a new member of the PEBP family of self discovery in cDNA Library of human phosphatidylethanolamine binding protein 4 (human phosphatidylethanolamine (PE) -bindign protein 4, hPEBP4) and prove its high expression in breast cancer and play the basic role of anti apoptotic, we cloned hPEBP4 mouse homologous molecule mPEBP4, and preliminary study on the biological function.
The first part, cloning and sequence analysis of mPEBP4, tissue cell distribution and cell localization
As mentioned above, PEBP is a kind of phosphatidylethanolamine binding protein (PE), all members of the family contain the conserved region combined with PE (PBP). Human phosphatidylethanolamine binding protein 4 (human phosphatidylethanolamine (PE) -binding protein 4, hPEBP4) is from human bone marrow stromal cell (BMSC) is a new member of the PEBP family are found in the cDNA library. The full-length cDNA is 874bp, encoding 227 amino acid protein. Our previous studies showed that the protein nucleic acid and amino acid in water

【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R392

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