本文關(guān)鍵詞：病毒吸附對宿主細(xì)胞膜的影響及中藥的干預(yù)作用　出處：《中國中醫(yī)科學(xué)院》2006年碩士論文　論文類型：學(xué)位論文

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【摘要】：病毒性疾病的危害已威脅到人類生存,在2006年3月由國際醫(yī)學(xué)科學(xué)院組織召開的第二屆全球大會上,500名專家指出:不斷變化的病毒使全球處于大流行的危險之中。WHO等單位聯(lián)合發(fā)布的《發(fā)展中國家疾病控制優(yōu)先項目》中指出:人類衛(wèi)生面臨的四大挑戰(zhàn),其中第三項為新發(fā)病毒性疾病的大流行。 隨著分子生物學(xué)理論和技術(shù)在病毒學(xué)研究中的應(yīng)用,盡管現(xiàn)代醫(yī)學(xué)對病毒感染機制的認(rèn)識有了飛越性的發(fā)展,但控制手段始終沒有突破性進展,尋找有效的控制病毒性疾病的手段已成為21世紀(jì)人類的重要事件。 中醫(yī)藥防治病毒性疾病有數(shù)千年的經(jīng)驗和完整的理論體系,是中醫(yī)藥治病的優(yōu)勢領(lǐng)域。以其療效確切、相對廣譜、低毒的特點已被世人所接受,醫(yī)學(xué)界期待從中醫(yī)藥中發(fā)現(xiàn)控制病毒性疾病的有效藥物。近20年來,雖然大量的試驗證實了中藥治療病毒性疾病的有效,尤其是SARS以后國內(nèi)外從中藥中尋找有效的抗病毒成分成為熱點,但直至目前抗病毒中藥的研制并沒有突破性進展。其原因①缺乏充分體現(xiàn)中醫(yī)藥治療病毒性疾病特點的研究思路;②缺乏充分體現(xiàn)中醫(yī)藥治療病毒性疾病特點的研究手段。因此,在中醫(yī)藥治療病毒性疾病理論的指導(dǎo)下,應(yīng)用現(xiàn)代生物學(xué)技術(shù)和手段研究中藥治療病毒性疾病的機理,對中藥走向世界具有重要的現(xiàn)實意義。 本研究在原有抗病毒藥效試驗和抗病毒作用機理研究的基礎(chǔ)上,從病毒吸附環(huán)節(jié)的一些非特異性病理生理環(huán)節(jié)入手,以2～3種病毒株為樣本,進一步探討病毒吸附細(xì)胞的生物學(xué)機制和中藥梔子提取物ZG和半枝蓮總黃酮對此環(huán)節(jié)的干預(yù)作用,為尋找中藥抗病毒作用的真正靶點奠定基礎(chǔ)。本論文的研究內(nèi)容為國家自然科學(xué)基金面上項目資助課題的部分內(nèi)容。 一、梔子提取物ZG及半枝蓮總黃酮的體外抗病毒作用研究 以Hep-2細(xì)胞株作為宿主細(xì)胞,以阿昔洛韋作為抗單純皰疹病毒1型的陽性對照藥,采用致細(xì)胞病變法(cytopathic effect CPE)來觀察梔子提取物ZG和半枝蓮總黃酮對4種病毒致細(xì)胞病變作用的影響,并按Reed-Muench方法計算最大無毒濃度(TC_0),50%細(xì)胞毒性濃度(TC_(50)),50%抑制濃度(IC_(50))和治療指數(shù)(TI)。結(jié)果顯示:梔子提取物
[Abstract]:The harm of viral diseases has threatened the survival of human beings at the second Global Congress organized by the International Academy of Medical Sciences in March 2006. 500 experts noted that the changing virus placed the world at risk of a pandemic. The World Health Organization (WHO), among others, reported in the priority for Disease Control in developing countries that:. Four major challenges to human health. The third is a pandemic of new viral diseases. With the application of molecular biological theory and technology in virology research, although the understanding of viral infection mechanism in modern medicine has developed by leaps and bounds, there has been no breakthrough in control methods. To find effective means to control viral diseases has become an important event in 21th century. Chinese medicine has thousands of years of experience in preventing and treating viral diseases and a complete theoretical system, which is the dominant field of TCM treatment. Because of its effective, relatively broad spectrum, low toxicity has been accepted by the world. The medical profession is looking forward to the discovery of effective drugs for the control of viral diseases from Chinese medicine. In the past 20 years, although a large number of experiments have proved the effectiveness of Chinese medicine in the treatment of viral diseases. Especially after SARS, it has become a hot spot to look for effective antiviral components from traditional Chinese medicine at home and abroad. However, until now, there has been no breakthrough in the development of antiviral Chinese medicine. The reasons are: 1 lack of research ideas to fully reflect the characteristics of traditional Chinese medicine in the treatment of viral diseases; 2 lack of research means to fully reflect the characteristics of traditional Chinese medicine in the treatment of viral diseases. Therefore, under the guidance of the theory of traditional Chinese medicine treatment of viral diseases. The application of modern biological techniques and means to study the mechanism of traditional Chinese medicine in treating viral diseases is of great practical significance to the development of traditional Chinese medicine in the world. On the basis of the original antiviral effect test and the mechanism of antiviral action, this study began with some non-specific pathophysiological links of virus adsorption, and took three virus strains as samples. To further explore the biological mechanism of virus adsorption cells and the effect of ZG and total flavonoids of Gardenia jasminoides on this link. In order to find the real target of antiviral effect of traditional Chinese medicine, the research content of this paper is part of the project supported by the National Natural Science Foundation of China. Antiviral effects of Gardenia jasminoides extract ZG and total flavonoids of Scutellaria barbata in vitro Hep-2 cell line was used as host cell and acyclovir as positive control drug against herpes simplex virus type 1. Cytopathic effect was used to observe the effects of Gardenia jasminoides extract ZG and total flavonoids of Scutellaria barbata on cytopathic effects of four viruses. The maximum nontoxic concentration (TC0%) was calculated by Reed-Muench method. 50% inhibitory concentration (ICS) and treatment index (TI). The results showed that the extract of Gardenia jasminoides was extracted from Gardenia jasminoides.
【學(xué)位授予單位】：中國中醫(yī)科學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R373;R285

【引證文獻】

相關(guān)期刊論文 前1條

1 徐玉鳳;劉家國;趙彪;王德云;范云鵬;胡元亮;徐樹培;廖敬宜;;板藍(lán)根生物堿酸性部位對新城疫病毒吸附和釋放的影響[J];南京農(nóng)業(yè)大學(xué)學(xué)報;2010年06期

相關(guān)碩士學(xué)位論文 前2條

1 廖軼;禽康散在CEF內(nèi)抗NDV效果及對SPL增殖和NO分泌影響的研究[D];四川農(nóng)業(yè)大學(xué);2010年

2 徐玉鳳;芪藍(lán)抗毒飲及其組方成分體外抗新城疫病毒作用及其機制初步研究[D];南京農(nóng)業(yè)大學(xué);2009年

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本文編號：1422783