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  本文關(guān)鍵詞：臍帶血細(xì)胞CD35與CXCR4在天然免疫反應(yīng)中的變化規(guī)律及其意義　出處：《第二軍醫(yī)大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 臍帶血 天然免疫 補(bǔ)體 趨化因子受體 紅細(xì)胞 淋巴細(xì)胞 粒細(xì)胞 CD35 CXCR4

【摘要】：目的:比較臍帶血細(xì)胞組與成人外周血細(xì)胞組天然免疫功能及各組紅細(xì)胞對白細(xì)胞天然免疫功能調(diào)控能力的差異。方法:通過腫瘤血細(xì)胞花環(huán)實(shí)驗(yàn)測定臍帶血細(xì)胞與成人外周血細(xì)胞的天然免疫黏附腫瘤細(xì)胞能力變化的指標(biāo),以及通過流式細(xì)胞儀法測定臍帶血細(xì)胞與成人血細(xì)胞分別在全血細(xì)胞添加腫瘤細(xì)胞、全血細(xì)胞添加生理鹽水、全血細(xì)胞去除紅細(xì)胞后添加腫瘤細(xì)胞、全血細(xì)胞去除紅細(xì)胞后添加生理鹽水四種情況下CD35、CXCR4的熒光強(qiáng)度。結(jié)果:臍帶血紅細(xì)胞腫瘤黏附率、淋巴細(xì)胞腫瘤黏附率、粒細(xì)胞腫瘤黏附率分別低于對應(yīng)正常成人組,統(tǒng)計學(xué)有差異;同樣條件下成人紅細(xì)胞CD35熒光強(qiáng)度要低于臍血組,而成人淋巴細(xì)胞、粒細(xì)胞CD35、CXCR4熒光強(qiáng)度要高于臍血組;成人與臍血淋巴細(xì)胞、粒細(xì)胞在添加了紅細(xì)胞后CD35、CXCR4熒光強(qiáng)度要高于未添加紅細(xì)胞組。結(jié)論:臍帶血細(xì)胞天然免疫功能較健康成人外周血細(xì)胞天然免疫功能低;成人外周血及臍帶血細(xì)胞中紅細(xì)胞對白細(xì)胞的天然免疫功能皆有增強(qiáng)作用,在添加了腫瘤細(xì)胞等抗原刺激物表現(xiàn)更明顯,其中臍帶血紅細(xì)胞的調(diào)控能力較正常成人外周血紅細(xì)胞的調(diào)控能力要弱。
[Abstract]:Objective: to compare the difference of innate immune function between cord blood cell group and adult peripheral blood cell group and the regulation ability of red blood cell to leukocyte innate immune function. The changes of the ability of cord blood cells and adult peripheral blood cells to adhere to tumor cells were measured by rosette assay. Umbilical cord blood cells and adult blood cells were added tumor cells to whole blood cells, normal saline to whole blood cells, and tumor cells were added to whole blood cells after removing red blood cells by flow cytometry. The fluorescence intensity of CD35 and CXCR4 in the whole blood cells after removing red blood cells and adding normal saline. Results: the adhesion rate of cord blood red blood cell tumor and lymphocyte tumor. The adhesion rate of granulocyte tumor was lower than that of the corresponding normal adult group (P < 0.05). Under the same condition, the fluorescence intensity of CD35 in adult erythrocytes was lower than that in cord blood group, while the fluorescence intensity of CD35 and CXCR4 in adult lymphocytes was higher than that in cord blood group. In adults with cord blood lymphocytes, granulocytes were added with red blood cells after CD35. Conclusion: the innate immune function of cord blood cells is lower than that of healthy adult peripheral blood cells. Red blood cells in adult peripheral blood and umbilical cord blood cells have enhanced the innate immune function of leukocytes, especially in the addition of tumor cells and other antigenic stimulators. The control ability of umbilical cord blood red blood cell is weaker than that of normal adult peripheral blood red blood cell.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R392.1

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