發(fā)布時(shí)間：2018-01-13 11:54

  本文關(guān)鍵詞：大鼠下丘腦細(xì)胞分離培養(yǎng)及體外分泌內(nèi)啡肽的實(shí)驗(yàn)研究　出處：《天津醫(yī)科大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 下丘腦 細(xì)胞培養(yǎng) β-內(nèi)啡肽 藥物成癮

【摘要】：藥物成癮已成為人們普遍關(guān)注的社會(huì)和醫(yī)學(xué)難題。國外有學(xué)者認(rèn)為,藥物依賴是一種腦病,且與高血壓、哮喘、Ⅱ型糖尿病三個(gè)公認(rèn)的慢性病相比,在遺傳性、個(gè)人責(zé)任、反復(fù)發(fā)作、治療反應(yīng)等方面有許多相似之處。因此藥物依賴是一種需要醫(yī)藥治療的慢性疾病。近來研究發(fā)現(xiàn),藥物成癮是由于外源性的使用阿片類藥物而抑制了體內(nèi)神經(jīng)元分泌內(nèi)啡肽,因而一旦停藥就可因體內(nèi)的內(nèi)啡肽下降而出現(xiàn)心理和生理的戒斷反應(yīng)。這已成為藥物依賴的重要機(jī)制之一。隨著細(xì)胞移植技術(shù)的發(fā)展,如果能將體外培養(yǎng)的具有穩(wěn)定分泌β-內(nèi)啡肽的細(xì)胞移植入藥物成癮者體內(nèi),植入的細(xì)胞就象一個(gè)“生物泵”源源不斷的產(chǎn)生β-內(nèi)啡肽,代償由于藥物依賴而產(chǎn)生的β-內(nèi)啡肽分泌不足,使機(jī)體β-內(nèi)啡肽達(dá)到正常水平,獲得心理和生理的滿足。這將成為治療藥物成癮最理想的方法。本研究通過實(shí)驗(yàn):1)建立大鼠下丘腦細(xì)胞分離和培養(yǎng)方法,觀察體外培養(yǎng)的大鼠下丘腦細(xì)胞的生長、成熟、停滯的形態(tài)學(xué)特點(diǎn)和免疫細(xì)胞學(xué)鑒別;2)通過ELISA法,測(cè)定體外培養(yǎng)的大鼠下丘腦細(xì)胞分泌的β-內(nèi)啡肽含量,了解下丘腦細(xì)胞分泌β-內(nèi)啡肽的自身調(diào)節(jié)功能,進(jìn)一步推斷阿片類藥物的成癮機(jī)制;3)通過對(duì)體外培養(yǎng)的下丘腦細(xì)胞的凍存-復(fù)蘇,了解其形態(tài)和分泌β-內(nèi)啡肽功能的改變,探討細(xì)胞移植治療藥物成癮
[Abstract]:Drug addiction has become a common social and medical problem. Some foreign scholars believe that drug dependence is a brain disease, and compared with hypertension, asthma, type 2 diabetes in the three recognized chronic diseases, in the genetic nature. There are many similarities in personal responsibilities, recurring episodes, and therapeutic responses. Drug dependence is therefore a chronic disease that requires medical treatment. Recent research has found. Drug addiction is due to the exogenous use of opiates to inhibit the production of endorphins in neurons. Therefore, once the drug is stopped, psychological and physiological withdrawal can occur due to the decrease of endorphin in the body. This has become one of the important mechanisms of drug dependence. With the development of cell transplantation technology. If cultured cells with stable 尾 -endorphins are transplanted into drug addicts, the implanted cells are like a "biological pump" that keeps producing 尾 -endorphins. Compensatory 尾 -endorphin secretion due to drug dependence is insufficient, making the body 尾 -endorphin to the normal level. To obtain psychological and physiological satisfaction. This will be the best way to treat drug addiction. In this study, we established a method for the separation and culture of rat hypothalamic cells by experiment: 1. The growth, maturation and stagnation of rat hypothalamic cells in vitro were observed. 2) the content of 尾 -endorphin secreted by rat hypothalamic cells in vitro was measured by ELISA method to understand the self-regulation function of 尾 -endorphin secreted by hypothalamic cells. Further infer the mechanism of opioid addiction; 3) through cryopreservation and resuscitation of hypothalamic cells cultured in vitro, to understand the changes of morphology and 尾 -endorphin secretion, and to explore the treatment of drug addiction by cell transplantation.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R329

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