本文關(guān)鍵詞：雄激素對去勢大鼠下丘腦AR和NGF表達(dá)的影響　出處：《西北農(nóng)林科技大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 雄激素 AR NGF 下丘腦 脊髓 嗅腦

【摘要】： 為了探討雄激素與腦組織之間的作用關(guān)系,本實(shí)驗運(yùn)用超敏感的免疫組織化學(xué)SP法,以SD大鼠為研究對象并建立去睪丸大鼠模型,通過補(bǔ)充外源性雄激素,觀察脊髓頸膨大、下丘腦、嗅腦中AR和NGF的表達(dá)變化,探討雄激素對腦中這兩種物質(zhì)的影響及其表達(dá)變化,研究雄激素對這些組織區(qū)域的保護(hù)機(jī)制。 1.在脊髓頸膨大中,AR和NGF免疫陽性產(chǎn)物廣泛分布于脊髓的灰質(zhì)中,而在白質(zhì)中未見其陽性表達(dá)。在灰質(zhì)中尤其以處于灰質(zhì)背側(cè)角的陽性細(xì)胞最為明顯。AR陽性產(chǎn)物主要定位于細(xì)胞胞漿、胞膜和突起中,但核著色不明顯。表明AR和NGF在脊髓中發(fā)揮了作用。但切除雙側(cè)睪丸后,AR表達(dá)強(qiáng)度沒有明顯下降,而NGF陽性表達(dá)強(qiáng)度則明顯下降,注射外源性雄激素后,NGF陽性表達(dá)強(qiáng)度則恢復(fù)到假手術(shù)組水平。 2.AR和NGF在下丘腦中廣泛分布,在下丘腦中的蒼白球、弓狀核、視前大細(xì)胞核、視前內(nèi)側(cè)核、視前外側(cè)核、視上核、室旁核、室周核、下丘腦腹內(nèi)側(cè)核、下丘腦前核、下丘腦外側(cè)核、終紋核等核團(tuán)中均可見到AR和NGF的陽性表達(dá)。其陽性表達(dá)也主要定位于胞膜、胞質(zhì)、突起中,在細(xì)胞核中僅有少量陽性表達(dá)。大鼠去睪丸后,體內(nèi)內(nèi)源性雄激素來源切斷,下丘腦中大多核團(tuán)AR和NGF陽性表達(dá)強(qiáng)度下降,在補(bǔ)充外源性雄激素后,上述變化被逆轉(zhuǎn),各核團(tuán)中陽性細(xì)胞數(shù)目和陽性表達(dá)率均有所恢復(fù)。但蒼白球中在切斷內(nèi)源性雄激素后,AR陽性表達(dá)強(qiáng)度有所上升,補(bǔ)充外源性雄激素后,陽性的表達(dá)強(qiáng)度有所下降。而觀察的核團(tuán)中,發(fā)現(xiàn)視上核在切斷內(nèi)源性雄激素后陽性細(xì)胞表達(dá)強(qiáng)度未見有大的變化,而在補(bǔ)充外源性雄激素后,依舊未見其陽性表達(dá)強(qiáng)度變化。 3.AR和NGF在嗅腦中也有廣泛的分布,在嗅腦的僧帽細(xì)胞、嗅前核內(nèi)側(cè)部、內(nèi)顆粒層、嗅結(jié)節(jié)叢狀層等部位均可以觀察到AR和NGF陽性細(xì)胞的表達(dá)。主要定位于胞漿和胞膜,在突起中也有少量分布。SD大鼠在去勢后,由于缺乏內(nèi)源性雄激素,嗅結(jié)節(jié)叢狀層、嗅結(jié)節(jié)背側(cè)部等區(qū)域AR和NGF陽性表達(dá)強(qiáng)度明顯下降,在經(jīng)補(bǔ)充雄激素治療后,兩種蛋白表達(dá)強(qiáng)度明顯回升與假手術(shù)組相當(dāng)。在嗅小球等部位,切斷內(nèi)源性雄激素后未見陽性細(xì)胞的表達(dá),在補(bǔ)充外源性雄激素后,亦未見陽性細(xì)胞表達(dá)。
[Abstract]:In order to study the relationship between androgen and brain tissue, this experiment used the super-sensitive immunohistochemical SP method to establish the testicular rat model with SD rats as the object of study, through the supplement of exogenous androgen. To observe the changes of AR and NGF expression in cervical enlargement, hypothalamus and olfactory brain, to investigate the effects of androgen on these two substances and their expression, and to study the protective mechanism of androgen on these tissue regions. 1. AR and NGF immunoreactive products were widely distributed in the gray matter of spinal cord in cervical enlargement. In the gray matter, especially in the dorsal horn of gray matter, the positive products of AR were mainly located in the cytoplasm, membrane and process. But the nuclear staining was not obvious, which indicated that AR and NGF played an important role in spinal cord. However, after bilateral testicular resection, the expression of AR did not decrease significantly, but the positive expression of NGF decreased significantly. After injection of exogenous androgen, the positive expression of NGF returned to the level of sham operation group. 2. AR and NGF were widely distributed in hypothalamus, including globus pallidus, arcuate nucleus, preoptic nucleus, medial preoptic nucleus, anterolateral optic nucleus, supraoptic nucleus, paraventricular nucleus and periventricular nucleus. The positive expression of AR and NGF in ventromedial hypothalamic nucleus, anterior hypothalamic nucleus, lateral hypothalamic nucleus and stria terminalis nucleus can be seen. The positive expression of AR and NGF is mainly located in the membrane, cytoplasm and process. After removing testis, the endogenous androgen sources were cut off, and the intensity of AR and NGF expression in the hypothalamus decreased, after the supplementation of exogenous androgen. These changes were reversed, the number of positive cells and the positive expression rate in each nucleus recovered to some extent, but the intensity of AR positive expression in globus pallidus increased after transection of endogenous androgen, and after supplement of exogenous androgen. The positive expression intensity decreased. In the observed nuclei, there was no significant change in the expression intensity of the supraoptic nucleus after transection of endogenous androgen, but after the supplementation of exogenous androgen, there was no significant change in the expression intensity of the positive cells. There was no change in positive expression intensity. 3. AR and NGF were also widely distributed in olfactory brain, including mitral cells, medial part of olfactory anterior nucleus and inner granular layer of olfactory brain. The expression of AR and NGF positive cells were observed in the plexiform layer of olfactory nodules, mainly located in the cytoplasm and membrane, and a few distributed in the process. SD rats were ovariectomized. Due to the lack of endogenous androgen, the positive expression of AR and NGF in the plexiform layer of olfactory nodules and the dorsal part of the olfactory nodules decreased significantly, and the positive intensity of AR and NGF decreased significantly after androgen supplementation. The expression intensity of the two proteins was similar to that of the sham-operated group. No positive cells were found in the olfactory pellets after transection of endogenous androgen, and no positive cells were found after the supplementation of exogenous androgen.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R363

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 喬海蓮;LIF、AR和PR在妊娠大鼠下丘腦、垂體、卵巢和子宮中的表達(dá)[D];西北農(nóng)林科技大學(xué);2008年

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本文編號：1414745