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  本文關鍵詞：胚胎小體制備新方法的建立及小鼠胚胎干細胞體外分化形成新生血管的實驗研究　出處：《大連醫(yī)科大學》2006年碩士論文　論文類型：學位論文

  更多相關文章： 胚胎干細胞 胚胎小體 分化 內(nèi)皮細胞 血管生成 血管新生

【摘要】： 研究背景和目的 胚胎干細胞(embryonic stem cells, ES cells)是從胚胎早期內(nèi)細胞團(inner cell mass, ICM)分離得到的全能干細胞,在體外培養(yǎng)時如去除飼養(yǎng)層細胞和白血病抑制因子( leukemia inhibitory factor, LIF)等維持干細胞未分化狀態(tài)的培養(yǎng)條件,ES細胞可自發(fā)形成具有內(nèi)、中、外三胚層結構的胚胎小體(embryoid bodies, EBs)。作為ES細胞體外分化的理想模型,EBs可以再現(xiàn)胚胎早期發(fā)育過程中的譜系特異性分化程序及基因時空表達模式的諸多方面,因此被廣泛用于研究胚層間相互誘導、器官空腔化形成等胚胎早期發(fā)育事件。此外,在由ES細胞向特定組織細胞誘導分化的過程中,往往需要經(jīng)過EBs階段的培養(yǎng),以完成ES細胞分化的啟動。由此可見,EBs制備已成為干細胞研究領域不可或缺的工具。目前常用的EBs制備方法,如懸滴培養(yǎng)法、懸浮培養(yǎng)法、甲基纖維素半固體培養(yǎng)法等,均存在著操作繁瑣、EBs產(chǎn)生數(shù)量少、大小和分化階段不一致等缺點,直接影響了相關研究的可行性及結果的可靠性。為改進EBs制備過程中存在的上述問題,本實驗通過采用先貼壁培養(yǎng)ES細胞3 d,再懸浮培養(yǎng)的策略,探討建立一種制備EBs的新方法。 近年來隨著癌癥和心腦血管疾病的增多,人們越來越關注血管形成機制的研究。充分認識這一過程,將對惡性腫瘤及缺
[Abstract]:Background and purpose of the study Embryonic stem cells (es cells) are derived from inner cell mass in early embryo. In vitro culture, such as the removal of feeder layer cells and leukemia inhibitory factor. Embryo embryoid bodies with inner, mesoderm and ectodermal structure can be formed spontaneously under the condition of maintaining the undifferentiated state of stem cells, such as LIFs. As an ideal model for es cell differentiation in vitro, EBs can reproduce the pedigree specific differentiation process and gene expression pattern in early embryonic development. Therefore, it has been widely used to study early embryonic development events such as interdermal induction, organ cavity formation and so on. In addition, in the process of inducing differentiation from es cells to specific tissue cells. It is often necessary to culture es cells through EBs stage to complete the initiation of es cell differentiation. Thus, the preparation of EBs has become an indispensable tool in the field of stem cell research. At present, EBs preparation methods are commonly used. For example, suspension culture method, suspension culture method, methyl cellulose semi-solid culture method and so on, there are some shortcomings such as less production of EBs in operation, inconsistent size and differentiation stage, and so on. In order to improve the above problems in the process of EBs preparation, we adopted the strategy of culture es cells for 3 days and then suspension culture. To establish a new method for preparing EBs. In recent years, with the increase of cancer and cardiovascular and cerebrovascular diseases, people pay more and more attention to the study of angiogenesis mechanism.
【學位授予單位】：大連醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R329

【參考文獻】

相關期刊論文 前2條

1 孫志杰,羅桂芬,李青旺;應用微囊化技術大量擴增擬胚體[J];中國畜牧獸醫(yī);2005年03期

2 韓雅玲,齊巖梅,康建,梁明,陳興華;絲裂原活化蛋白激酶在人冠狀動脈平滑肌細胞向三維纖維蛋白膠遷移中的作用[J];中國應用生理學雜志;2005年04期

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本文編號：1412283