本文關鍵詞：日本血吸蟲（云南品系）未成熟卵可溶性抗原免疫保護性觀察　出處：《昆明醫(yī)學院》2005年碩士論文　論文類型：學位論文

  更多相關文章： 日本血吸蟲 血吸蟲病 卵 抗病免疫 抗卵胚發(fā)育 抗生殖產卵

【摘要】：[目的] 對日本血吸蟲云南品系未成熟卵可溶性抗原(soluble immature eggs antigen,SIEA)免疫鼠體內日本血吸蟲成蟲的睪丸及卵黃腺組織進行超微結構觀察,探討SIEA抗日本血吸蟲生殖作用機制;觀察日本血吸蟲云南品系未成熟卵可溶性抗原(SIEA)的抗病免疫作用;觀察小鼠經(jīng)云南品系SIEA免疫后用皖鄂品系血吸蟲尾蚴攻擊感染,是否也能誘導產生抗病免疫效果。 [方法] 制備日本血吸蟲云南品系未成熟卵可溶性抗原(SIEA)。取適量SIEA加等量福氏佐劑,背部皮下多點注射免疫ICR小鼠后分2組,分別用大理釘螺和華東釘螺逸出的血吸蟲尾蚴(30±2條)攻擊感染。于感染后46天剖殺小鼠,觀察減蟲率、糞卵減少率、雌蟲子宮內蟲卵數(shù)、肝表面蟲卵結節(jié)數(shù)、肝臟各期蟲卵數(shù)及各期蟲卵構成比;取小鼠肝臟按常規(guī)制作病理切片,觀察肝組織內肉芽腫病變情況并分析肉芽腫平均直徑;取合抱成蟲,分離出雌蟲卵黃腺和雄蟲睪丸組織進行超微結構觀察。 [結果] SIEA免疫后減蟲效果不明顯,大理組與華東組糞卵減少率分別為33.25%、34.45%(P0.05),雌蟲子宮內蟲卵數(shù)減少率分別為34.75%、23.69%(P0.05),肝表面蟲卵結節(jié)密度分別下降47.76%、29.03%(P0.05),肝蟲卵肉芽腫大小比對照組分別下降了49.06%、30.69%(P0.05);肝組織內蟲卵總數(shù)下降,大理組與華東組每雌肝卵減少率分別為
[Abstract]:[Objective] to Yunnan strains of Schistosoma japonicum soluble immature egg antigen (soluble immature eggs antigen, SIEA) on the ultrastructure of mice immunized Schistosoma japonicum adult testis and yolk gland tissue, SIEA of Schistosoma japonicum reproductive mechanism; observe the Yunnan strain of Schistosoma japonicum soluble immature egg antigen (SIEA) immune disease effect; mice immunized with SIEA Yunnan strains with Anhui and Hubei strains of schistosoma cercariae infection, can induce the immune effect of resistance.
[method] the preparation of Yunnan strains of Schistosoma japonicum soluble immature egg antigen (SIEA). Apply SIEA with equal volume of Freund's adjuvant. Subcutaneous multi-point injection of immune ICR mice were divided into 2 groups, respectively in Dali and East China from Oncomelania snails schistosoma cercariae (30 + 2) infection after infection 46 attack. The day the mice were sacrificed and the observation of the worm reduction rate, fecal egg reduction rate, the number of eggs within the female uterus, liver surface egg nodule number, the number of eggs in liver ratio and different stages of egg; mice liver by routine pathological sections were made, were observed in liver tissue and granuloma granuloma analysis of average diameter; take the paring adult isolated, ultrastructure of testicular tissue of the vitelline gland of female and male.
[results] SIEA after immunization with the worm reduction effect is not obvious, Dali group and East Group fecal egg reduction rates were 33.25%, 34.45% (P0.05), the number of eggs within the female uterus reduction rate were 34.75%, 23.69% (P0.05), the surface of the liver egg nodule density decreased by 47.76%, 29.03% (P0.05), hepatic granuloma size compared with the control group decreased by 49.06%, 30.69% (P0.05); liver eggs in the decline in the total number of Dali group and East Group, each female liver egg reduction rates were

【學位授予單位】：昆明醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R392

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