本文關(guān)鍵詞：基于分子和細胞水平的微流控芯片診斷研究　出處：《中國科學(xué)院研究生院（大連化學(xué)物理研究所）》2005年博士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 微流控芯片 基因診斷 基因分析 細胞凋亡

【摘要】：微流控芯片或芯片實驗室是系統(tǒng)生物學(xué)研究的重要技術(shù)平臺之一。本論文以微流控芯片為技術(shù)依托,從具有臨床意義的疾病診斷出發(fā),對微流控芯片在基因分析和細胞凋亡等領(lǐng)域的應(yīng)用進行了一系列具有一定覆蓋面的研究,力圖發(fā)展具有潛在生物醫(yī)學(xué)應(yīng)用價值的相關(guān)技術(shù)。論文共分六章�！� 第一章闡述了微流控芯片在基因分析和細胞凋亡領(lǐng)域研究的進展�！� 第二～五章集中探討了利用實驗室自行設(shè)計、研制的微流控芯片系統(tǒng),開展以基因多態(tài)性分析、外源性基因檢測和基因表達為主要途徑的基因分析的可行性。主要包括:1)大連地區(qū)123 例高血壓患者血管緊張素原基因啟動子區(qū)A-6G基因多態(tài)性分析;2)人參微衛(wèi)星位點短串聯(lián)重復(fù)序列多態(tài)性分析,以及在此基礎(chǔ)上的東方參和西洋參鑒別;3) 臨床常見病原體結(jié)核桿菌、丙型肝炎病毒基因PCR 產(chǎn)物和人類白細胞相關(guān)抗原(HLA-B27)基因的快速檢測;和4)細胞RNA完整性評價及不同組織巖藻糖基轉(zhuǎn)移酶基因表達分析。 鑒于細胞凋亡這一生物學(xué)過程在疾病診斷和治療上的重要意義,論文第六章以氧化砷誘導(dǎo)急性早幼粒白血病細胞凋亡為模型,以細胞氧化還原狀態(tài)變化和DNA 片段化等凋亡事件為主要研究內(nèi)容,實現(xiàn)了對凋亡白血病細胞內(nèi)活性氧和還原型谷胱甘肽的同時測定以及DNA 片段化分析,顯示了微流控芯片平臺用于細胞凋亡研究的良好前景�！�
[Abstract]:Microfluidic chip or chip laboratory is one of the important technology platform for systems biology research. In this paper, the microfluidic chip technology, starting from the diagnosis of clinical significance, the application of microfluidic chip in gene analysis in the field have carried out a series of certain coverage of the study, in order to develop the related techniques with potential biomedical application value. This dissertation consists of six chapters.
The first chapter describes the progress of microfluidic chips in the field of gene analysis and cell apoptosis.
Second to the five chapter discusses the use of self-designed laboratory, microfluidic chip system development, to carry out genetic polymorphism analysis. The expression of exogenous gene detection and gene feasibility analysis as the main pathway. Mainly includes: 1) 123 cases of hypertension patients in Dalian region of angiotensinogen gene analysis of the promoter region of A-6G gene polymorphism; 2) repeat polymorphism analysis of microsatellite loci in short series, and on the basis of the Oriental ginseng and American ginseng identification; 3) fromtuberculosis, hepatitis C virus PCR gene product and human leukocyte antigen (HLA-B27) for rapid detection of gene expression analysis; and 4) enzyme gene transfer of RNA cells in different tissues and evaluate the integrity of fucosylation.
In view of the important significance of cell apoptosis in the biological process in the diagnosis and treatment of diseases, the sixth chapter of the thesis to arsenic induced apoptosis of acute promyelocytic leukemia cells as a model, the cellular redox state changes and DNA fragmentation of apoptotic events as the main research contents, the analysis on the determination of active oxygen in the apoptosis of leukemia cells at the same time, glutathione and DNA fragmentation, good prospects for apoptosis research of microfluidic chip platform for display.

【學(xué)位授予單位】：中國科學(xué)院研究生院（大連化學(xué)物理研究所）
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R346

【參考文獻】

相關(guān)期刊論文 前1條

1 林炳承;微流控芯片實驗室及其功能化[J];中國藥科大學(xué)學(xué)報;2003年01期

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本文編號：1377868