本文關(guān)鍵詞：腦缺血小鼠大腦皮層CD4、CD8免疫反應(yīng)觀察及脾T淋巴細(xì)胞亞群變化　出處：《大連醫(yī)科大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 腦缺血 免疫應(yīng)答 流式細(xì)胞儀 T淋巴細(xì)胞亞群

【摘要】： 背景和目的: 免疫機(jī)制與中樞神經(jīng)系統(tǒng)損傷、腫瘤、缺血以及退變性疾病密切相關(guān)。急性缺血性腦卒中是中樞神經(jīng)系統(tǒng)的急性損傷性病變,有研究表明,免疫機(jī)制參與了缺血性腦損傷的發(fā)生發(fā)展過程。腦缺血時(shí),激活的小膠質(zhì)細(xì)胞,浸潤(rùn)的外周血多形核白細(xì)胞、中性粒細(xì)胞和單核細(xì)胞等炎性細(xì)胞介入腦缺血性損傷的病理過程外,機(jī)體的免疫細(xì)胞水平也有變化。當(dāng)T淋巴細(xì)胞亞群的數(shù)量和功能異常時(shí),機(jī)體可產(chǎn)生免疫紊亂而導(dǎo)致機(jī)體發(fā)病。T細(xì)胞亞群與腦缺血的聯(lián)系近年來(lái)倍受重視。本實(shí)驗(yàn)研究的主要目的在于觀察腦缺血后腦組織淋巴細(xì)胞浸潤(rùn)情況及脾臟T細(xì)胞亞群的變化,探討淋巴細(xì)胞及其亞群在缺血性腦損傷中的作用。 方法: 研究對(duì)象為健康成年雄性Balb/c小鼠,光化學(xué)法建立小鼠局灶性腦缺血模型,分為對(duì)照組、單純光敏劑組和腦缺血組,各組又分為腦缺血后6h、1d、3d和7d四個(gè)亞組。在處死前半小時(shí),無(wú)菌條件下取出脾臟,制備單個(gè)核細(xì)胞懸液,用于流式細(xì)胞儀檢測(cè)脾臟CD4~+、CD8~+T淋巴細(xì)胞百分率及CD4~+/CD8~+的比值。在相應(yīng)時(shí)間點(diǎn)處死動(dòng)物,通過Nissl染色觀察受損腦區(qū)皮層的形態(tài)學(xué)變化,免疫組織化學(xué)染色檢測(cè)皮層CD4和CD8陽(yáng)性細(xì)胞的反應(yīng)。 結(jié)果: Nissl染色顯示:對(duì)照組和單純光敏劑組皮層無(wú)損傷,細(xì)胞分布和形態(tài)均正常,兩組之間無(wú)差異。與對(duì)照組相比,缺血組6h就出現(xiàn)損傷區(qū),1d和3d可見梗死區(qū)與周圍正常腦組織有一明顯的分界線,缺血周
[Abstract]:Background and purpose: Immune mechanism is closely related to central nervous system injury, tumor, ischemia and degenerative diseases. Acute ischemic stroke is an acute lesion of the central nervous system. Immune mechanism is involved in the occurrence and development of ischemic brain injury. During cerebral ischemia, activated microglia and infiltrated peripheral blood polymorphonuclear leukocytes. In addition to the involvement of inflammatory cells such as neutrophils and monocytes in the pathological process of ischemic brain injury, the level of immune cells in the body also changes, when the number and function of T lymphocyte subsets are abnormal. The relationship between the pathogenesis of T cell subsets and cerebral ischemia has been paid more and more attention in recent years. The main purpose of this experiment is to observe the infiltration of lymphocytes in brain tissue and spleen T after cerebral ischemia. Changes in cell subsets. To investigate the role of lymphocyte and its subsets in ischemic brain injury. Methods: The model of focal cerebral ischemia in healthy adult male Balb/c mice was established by photochemical method. The mice were divided into three groups: control group, simple Guang Min group and cerebral ischemia group. Each group was divided into 6 hours after cerebral ischemia. The spleen was taken out half an hour before the death and the mononuclear cell suspension was prepared for the detection of CD4 ~ by flow cytometry. The percentage of CD8T lymphocytes and the ratio of CD4- / CD8. the animals were killed at the corresponding time points, and the morphological changes of the damaged cerebral cortex were observed by Nissl staining. Immunohistochemical staining was used to detect the reaction of CD4 and CD8 positive cells in cortex. Results: Nissl staining showed that there was no damage to cortex, cell distribution and morphology were normal in the control group and Guang Min group, and there was no difference between the two groups. Compared with the control group, the injured area appeared at 6 hours in the ischemic group. On the 1st and 3rd days, there was a clear boundary between the infarcted area and the surrounding normal brain tissue, and the ischemic week.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊靜;黃帆;;細(xì)胞因子信號(hào)轉(zhuǎn)導(dǎo)抑制因子-3在缺血性腦血管病中的研究進(jìn)展[J];國(guó)際內(nèi)科學(xué)雜志;2007年10期

2 張位星;陳勝喜;羅萬(wàn)俊;;胸腔鏡胸腺擴(kuò)大切除治療重癥肌無(wú)力及圍術(shù)期處理[J];中南大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2009年07期

3 胡曉燕;陳葉;肖啟亮;;新生兒缺氧缺血性腦病體液sICAM-1水平與神經(jīng)行為評(píng)分的關(guān)系[J];南華大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2007年05期

4 魯文果;姜丹;彭軍;張?zhí)K明;;缺血后適應(yīng)對(duì)局灶性腦缺血再灌注大鼠外周血淋巴細(xì)胞DNA損傷的影響[J];臨床神經(jīng)病學(xué)雜志;2014年02期

5 余資江;應(yīng)大君;崔曉萍;董世武;;noggin基因修飾神經(jīng)干細(xì)胞移植對(duì)衰老小鼠學(xué)習(xí)記憶能力的改善[J];神經(jīng)解剖學(xué)雜志;2007年06期

6 郭文英;李瑞士;王瑋;董殿斌;汪愛英;;可溶性細(xì)胞間粘附分子-1在新生兒缺氧缺血性腦病血清中的表達(dá)及臨床意義[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2011年16期

7 劉婷婷;張s，

本文編號(hào)：1374317