本文關(guān)鍵詞：內(nèi)臟初級(jí)傳入神經(jīng)和化學(xué)表型的多重性　出處：《新疆醫(yī)科大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 腸神經(jīng)系統(tǒng) 內(nèi)臟傷害性刺激感受器 細(xì)胞內(nèi)鈣阻斷蛋白 植物凝集素B4

【摘要】： 目的:探討腸神經(jīng)系統(tǒng)的內(nèi)臟初級(jí)傳入神經(jīng)元形態(tài)和神經(jīng)元的化學(xué)表型,以此確定內(nèi)臟傷害性刺激感受器的神經(jīng)化超敏作用,為尋找傷害性刺激感受器的化學(xué)標(biāo)記物和探討內(nèi)臟感覺超敏的神經(jīng)機(jī)制。方法:利用免疫熒光細(xì)胞化學(xué)技術(shù)對(duì)BALBc小鼠的腸神經(jīng)系統(tǒng)小腸和結(jié)腸遠(yuǎn)端的粘膜下神經(jīng)叢(SMP)和肌間神經(jīng)叢(MP)的內(nèi)臟初級(jí)傳入神經(jīng)元進(jìn)行人類神經(jīng)蛋白(HU蛋白),細(xì)胞內(nèi)鈣阻斷蛋白(Calretinin)和植物凝集素B4(IB4)的化學(xué)標(biāo)記。結(jié)果:人類神經(jīng)蛋白能標(biāo)記所有的內(nèi)臟初級(jí)傳入神經(jīng)元,Hu蛋白被認(rèn)為是神經(jīng)元的常規(guī)陽性標(biāo)記物。顯示細(xì)胞體形態(tài)和胞體的全漿性表達(dá)。Calretinin免疫陽性反應(yīng)作為內(nèi)臟初級(jí)傳入神經(jīng)元的顯著標(biāo)記物。Calretinin能很好的顯示細(xì)胞的形態(tài),Calretinin標(biāo)記的顯著特點(diǎn)在于整個(gè)細(xì)胞以及樹突均勻地分布陽性反應(yīng)地標(biāo)記物。Calretinin標(biāo)記的陽性神經(jīng)元顯示細(xì)胞體和樹突,Hu蛋白標(biāo)記的神經(jīng)元表面光滑,而Calretinin免疫陽性神經(jīng)元表面有突起,免疫反應(yīng)陽性標(biāo)記部位從根本把兩者區(qū)分開。而植物凝集素B4免疫反應(yīng)陽性神經(jīng)元僅標(biāo)記細(xì)胞膜和胞內(nèi)顆粒狀物質(zhì)。IB4免疫陽性部位發(fā)生在細(xì)胞膜的表面和細(xì)胞內(nèi)一側(cè)的高爾基復(fù)合體,形成薄層的細(xì)胞膜表面陽性和胞漿內(nèi)一側(cè)的顆粒狀陽性物質(zhì)。IB4的細(xì)胞膜表達(dá)從根本上區(qū)別于Calretinin免疫反應(yīng)陽性的全漿性和胞體表面的多突起表達(dá)。三種不同的陽性標(biāo)記物,免疫反應(yīng)部位既有雷同又有區(qū)別,使得免疫反應(yīng)陽性神經(jīng)元的定性有了確切的保證。通過三種不同的陽性標(biāo)記物來發(fā)現(xiàn)和確認(rèn)內(nèi)臟傷害性刺激感受器的化學(xué)表型。結(jié)論:植物凝集素B4能與細(xì)胞膜上的碳糖類化合物特異性的結(jié)合,作為選擇性細(xì)胞靶標(biāo)來證實(shí)傷害性刺激信息由內(nèi)臟初級(jí)傳入神經(jīng)元的傳導(dǎo)。在小鼠的內(nèi)臟神經(jīng)系統(tǒng),IB4免疫反應(yīng)的陽性神經(jīng)元,能夠被確定為內(nèi)臟傷害性刺激感受器的初級(jí)傳入。IB4標(biāo)記的陽性神經(jīng)元對(duì)內(nèi)臟傷害性刺激的感受觸發(fā)了傷害性刺激過程的啟動(dòng)。內(nèi)臟的高敏性是腸易激綜合癥(IBS)的主要發(fā)病機(jī)制,腸道感覺的高敏性和初級(jí)傳入神經(jīng)沖動(dòng)地異常在IBS發(fā)病中起重要作用,胃腸動(dòng)力的變化被認(rèn)為是IBS最基本的病理生理學(xué)異常。在急性疼痛過程中,闡明了痛覺神經(jīng)元的高敏化機(jī)制。
[Abstract]:Objective: To investigate the chemical phenotype of the enteric nervous system visceral afferent neurons and neuron morphology, in order to determine the visceral nociceptive stimulation receptor nerve ultra sensitive function, neural system for chemical markers for nociception and discussion of visceral stimulation hypersensitivity. Methods: BALBc mice intestinal nervous system the small intestine and the distal colon by fluorescent immunocytochemistry in the submucosal plexus (SMP) and myenteric plexus (MP) of primary afferent neurons of human visceral nerve protein (HU protein), intracellular calcium blocker protein (Calretinin) and lectin B4 (IB4) chemical markers. Results: human neuroprotein can mark all the visceral afferent neurons, Hu protein is considered normal positive markers of neurons. Show the morphology and cell body expression of.Calretinin immunoreactivity in the cytoplasm Visceral primary afferent neurons were markers of.Calretinin can show the cell shape is very good, the remarkable characteristics of Calretinin labeled positive neurons and dendritic cells in the uniform distribution of positive markers of.Calretinin markers showed cell bodies and dendrites of neurons, surface Hu protein and Calretinin immunoreactive neurons in smooth surface processes. Immunoreactive markers to distinguish the two parts from the root. The Golgi complex side lectin B4 immunoreactive positive neurons only plant cell membrane and intracellular markers of.IB4 immune positive granular material occurs in the cell membrane surface and inside the cell, the cell membrane formed on the side surface of the cell membrane and cytoplasm positive in the granular layer the expression of.IB4 positive material fundamentally different from the Calretinin immunoreactive pulp and cell surface The expression of surface protrusions. Three different positive markers, immune response at the site of both identical and difference, the qualitative immunoreactive neurons have a guarantee. By three different positive markers to find and confirm the visceral nociceptive stimulation receptor phenotype. Conclusion: chemical plant lectin B4 carbon specific carbohydrate compounds combined with the cell membrane, as a selective target cell to confirm the nociceptive information from the visceral afferent neuron conduction. In the visceral nervous system in mice, IB4 immunoreactive positive neurons, can be identified as visceral noxious stimulation receptor positive neurons in primary afferent.IB4 mark of visceral nociceptive triggered nociceptive process started. Visceral hypersensitivity is irritable bowel syndrome (IBS) is the main pathogenesis of gut feeling The abnormality of high sensitivity and primary afferent nerve impulse plays an important role in the pathogenesis of IBS. The change of gastrointestinal motility is regarded as the most basic pathophysiological abnormality of IBS. In the process of acute pain, the hyperalgesia mechanism of pain neurons is elucidated.

【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R322

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