本文關(guān)鍵詞：小鼠Allo-HSCT后T淋巴細(xì)胞及其亞群組織分布的動態(tài)變化與GVHD關(guān)系　出處：《第二軍醫(yī)大學(xué)》2005年博士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 移植物抗宿主病 T淋巴細(xì)胞亞群 CD25 白介素-2

【摘要】：目的:深入研究T淋巴細(xì)胞亞群參與GVHD發(fā)生的機制,為防治GVHD的研究提供技術(shù)平臺和理論基礎(chǔ)。方法:用免疫磁珠陰性分選方法從轉(zhuǎn)eGFP基因的C57/BL小鼠脾細(xì)胞中分離CD4~+T淋巴細(xì)胞、CD8~+T淋巴細(xì)胞,并輸入經(jīng)致死量全身照射的BALB/C小鼠體內(nèi)制作GVHD模型。熒光顯微鏡觀察受體鼠組織中eGFP~+細(xì)胞的分布、擴增情況。流式細(xì)胞儀檢測T淋巴細(xì)胞表面分子,ELISA法檢測細(xì)胞因子的動態(tài)變化。結(jié)果:CD4~+T淋巴細(xì)胞或(和)CD8~+T淋巴細(xì)胞均可誘發(fā)GVHD。移植后供體T淋巴細(xì)胞在肝、皮膚、腸、肺等組織中均呈逐漸增多趨勢,而脾中呈逐漸減少趨勢。T淋巴細(xì)胞表面CD25及受體鼠外周血IL-2水平在移植早期短期高峰表達(dá),后迅速下降。結(jié)論:CD4~+T或CD8~+T淋巴細(xì)胞單獨均可誘發(fā)GVHD。T淋巴細(xì)胞進(jìn)入受體后先進(jìn)入淋巴器官,激活擴增再移出淋巴器官,進(jìn)入多種受體組織。IL-2及其受體CD25分子主要在移植早期參與GVHD。
[Abstract]:Objective: to investigate the role of T lymphocyte subsets in the pathogenesis of GVHD. Methods: CD4T lymphocytes were isolated from spleen cells of C57 / BL mice transformed with eGFP gene by immunomagnetic bead negative sorting method. CD8 ~ T lymphocytes were infused into BALB/C mice to make GVHD model. The distribution of eGFP~ cells in recipient mice was observed by fluorescence microscope. Amplification. Surface molecules of T lymphocytes were detected by flow cytometry. ELISA assay was used to detect the dynamic changes of cytokines. Results the donor T lymphocytes were in the liver and skin after transplantation. The expression of CD25 on the surface of T lymphocytes and IL-2 in peripheral blood of recipient mice was peak in the early stage of transplantation. Conclusion GVHD.T lymphocytes can be induced to enter the receptor and enter the lymphoid organs first by activation amplification and then out of the lymphoid organs by either CD4T or CD8T alone. IL-2 and its receptor CD25 molecules were mainly involved in the early stage of transplantation.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R392

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