本文關(guān)鍵詞：內(nèi)嗎啡肽在大鼠脊髓背角淺層調(diào)控外周傷害性信息傳遞的機制　出處：《第四軍醫(yī)大學》2005年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 內(nèi)嗎啡肽 鎮(zhèn)痛 脊髓背角 μ阿片受體 P物質(zhì) 電壓鉗記錄 大鼠

【摘要】：內(nèi)嗎啡肽-1(EM-1)和內(nèi)嗎啡肽-2(EM-2)是μ型阿片受體(MOR)的內(nèi)源性配體。MOR激活后能夠抑制腺苷酸環(huán)化酶,開啟膠狀質(zhì)(SG)神經(jīng)元上的鉀通道和抑制背根節(jié)神經(jīng)元的鈣電流。EMs和MOR在脊髓背角淺層內(nèi)均有大量的分布。脊髓背角淺層(Ⅰ、Ⅱ?qū)?是接受外周傷害性信息的初級門戶,傷害性信息在此中繼、整合后,由投射神經(jīng)元向上位腦結(jié)構(gòu)傳遞。P物質(zhì)(SP)是初級傳入纖維傳遞傷害性信息的重要神經(jīng)活性物質(zhì),脊髓背角淺層內(nèi)有密集的SP陽性纖維和終末,并且多數(shù)SP陽性纖維和終末也同時表達MOR。大量的實驗證據(jù)表明EMs在脊髓水平發(fā)揮明確的鎮(zhèn)痛作用,但是EMs在脊髓水平參與傷害性信息傳遞的細胞和分子機制仍不清楚。故本研究應(yīng)用脊髓薄片膜片鉗全細胞記錄技術(shù)觀察了EMs對成年大鼠SG內(nèi)興奮性和抑制性突觸傳遞的影響以及EMs在脊髓背角淺層內(nèi)對初級傳入終末釋放SP的影響,以期進一步闡明EMs在脊髓水平鎮(zhèn)痛作用的機制。 結(jié)果表明:(1) EMs抑制SG內(nèi)興奮性和抑制性突觸傳遞。EM-1(1 μmol/L)和EM-2(1 μmol/L)都能夠顯著抑制微小興奮性突觸后電流(mEPSCs)和微小抑制性突觸后電流(mIPSCs)的頻率而不改
[Abstract]:Endomorphine -1 (EM-1) and endomorphine -2 (EM-2) is opioid receptor (MOR) of the endogenous ligand.MOR can inhibit the activation of adenylate cyclase (SG), open the substantia gelatinosa neurons and inhibition of potassium channels in dorsal root ganglion neurons of calcium current.EMs and the distribution of MOR in spinal cord dorsal there are a lot of angle in the superficial layers. The superficial layers of the spinal dorsal horn (I, II layer) is the primary portal for peripheral nociceptive information, nociceptive information in the relay, after integration, by projecting neurons to the brain structure transfer of substance.P (SP) is an important neurotransmitter of primary afferent fibers transmit nociceptive signal the superficial layers of the spinal dorsal horn in the dense SP positive fibers and terminals, and the majority of SP positive fibers and terminals also express MOR. substantial experimental evidence indicates that EMs plays a significant analgesic effect at the spinal level, but the level of EMs in the spinal cord injury involved in the information transmission of fine The cellular and molecular mechanisms are still unclear. So the research and application of spinal cord slice patch clamp whole cell recording technique to observe the EMs of adult rat SG in excitatory and inhibitory synaptic transmission and the effect of EMs in the spinal dorsal horn effect on the release of SP from primary afferent terminals in the superficial layers, in order to further elucidate the mechanism of EMs in spinal cord the level of analgesia.
The results showed that: (1) EMs inhibited the excitatory and inhibitory synaptic transmission in SG,.EM-1 (1 mol/L) and EM-2 (1 mol/L) could significantly inhibit the frequency of small excitatory postsynaptic current (mEPSCs) and tiny inhibitory postsynaptic current (mIPSCs).

【學位授予單位】：第四軍醫(yī)大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R33

【共引文獻】

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1 朱建軍;;脊髓P物質(zhì)在電針鎮(zhèn)痛中作用的研究進展[J];南通大學學報(醫(yī)學版);2007年03期

2 冷冬妮,馮宇鵬,李云慶;內(nèi)嗎啡肽在脊髓背角淺層的鎮(zhèn)痛機制[J];中國神經(jīng)科學雜志;2004年03期

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1 陳濤;冷冬妮;李云慶;;內(nèi)嗎啡肽在中樞神經(jīng)系統(tǒng)發(fā)揮鎮(zhèn)痛作用的機理[A];神經(jīng)科學進展（三）論文集[C];2004年

相關(guān)碩士學位論文 前3條

1 惠瑞;大鼠下丘腦與孤束核間內(nèi)嗎啡肽陽性神經(jīng)元的往返聯(lián)系[D];第四軍醫(yī)大學;2006年

2 祁海軍;去部分背根及電針刺對貓脊髓Ⅱ板層SP表達的影響[D];昆明醫(yī)學院;2008年

3 呂伯昌;大鼠孤束核內(nèi)嗎啡肽能神經(jīng)元向臂旁核及中腦導水管周圍灰質(zhì)的投射[D];第四軍醫(yī)大學;2008年

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本文編號：1367034