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  本文關(guān)鍵詞：人未成熟卵母細胞體外培養(yǎng)成熟的研究　出處：《安徽醫(yī)科大學》2005年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 卵母細胞 體外成熟 人 表皮生長因子 人成熟卵泡液 體外培養(yǎng)成熟 體外受精 非整倍體 熒光原位雜交

【摘要】：目的 探討培養(yǎng)液中添加人成熟卵泡液(HFF)、表皮生長因子(EGF)對人未成熟卵母細胞體外成熟(IVM)、體外受精(IVF)及其胚胎發(fā)育的影響。 方法 未成熟卵母細胞來自接受IVM治療的多囊卵巢綜合癥(PCOS)患者。病人隨機分配到不同組別中:對照組:TCM199+10%SSS+0.075IU/ml FSH+0.51U/mlHCG+1μg/ml 17β-E_2+0.05mg/ml青霉素+0.075mg/ml鏈霉素+0.2mM/L丙酮酸;卵泡液組:對照組液+10%HFF:EGF組:對照組液+2ng/mlEGF。培養(yǎng)24-48小時后,對已排出第一極體的卵母細胞行卵胞漿內(nèi)單精子顯微注射(ICSI),注射后16-18h小時,觀察卵母細胞受精情況,受精后繼續(xù)培養(yǎng)2—3天行胚胎移植(ET),觀察成熟率、受精率、卵裂率、2細胞、8細胞、優(yōu)質(zhì)胚胎率及種植率、臨床妊娠情況。 結(jié)果 1、HFF、EGF對未成熟卵母細胞MII、受精及卵裂率的影響 HFF組與對照組相比,前者的卵裂率明顯高于后者,P0.01,而MII率、受精率差異無顯著性,P
[Abstract]:Objective to investigate the effect of human mature follicular fluid (HFF) and epidermal growth factor (EGF) on immature oocyte maturation (IVM), in vitro fertilization (IVF) and embryo development in vitro.
Methods immature oocytes from IVM treated with polycystic ovary syndrome (PCOS) patients. The patients were randomly assigned to different groups: the control group: TCM199+10%SSS+0.075IU/ml FSH+0.51U/mlHCG+1 g/ml -E_2+0.05mg/ml +0.075mg/ml +0.2mM/L 17 beta penicillin streptomycin pyruvic acid; follicular fluid group: control group group +10%HFF:EGF control group: liquid liquid +2ng/mlEGF. culture after 24-48 hours of discharge the first polar body oocytes by intracytoplasmic sperm injection (ICSI), 16-18h hours after injection, observation of oocytes, fertilization after cultured for 2 to 3 days of embryo transfer (ET), to observe the maturation rate, fertilization rate, cleavage rate, 2 cell, 8 cell, embryo quality the clinical pregnancy rate and implantation rate.
Result
1, the effect of HFF and EGF on the MII, fertilization and cleavage rate of immature oocytes were significantly higher than that of the control group. The cleavage rate of the former group was significantly higher than that of the control group, while the P0.01 rate, MII rate and fertilization rate had no significant difference between the HFF group and the control group, P.

【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R321

【引證文獻】

相關(guān)碩士學位論文 前2條

1 巫新春;人未成熟卵母細胞體外培養(yǎng)技術(shù)的臨床應(yīng)用及影響因素研究[D];安徽醫(yī)科大學;2006年

2 馮文娟;人黃體期卵母細胞的體外成熟及玻璃化冷凍的實驗研究[D];山東大學;2012年

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本文編號：1362893