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  本文關(guān)鍵詞：雙歧桿菌胞壁蛋白誘導(dǎo)人腸腺上皮細(xì)胞β-防御素-2基因表達及其信號轉(zhuǎn)導(dǎo)機制　出處：《四川大學(xué)》2005年博士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 防御素 誘導(dǎo)表達 雙歧桿菌 人腸道上皮細(xì)胞 信號轉(zhuǎn)導(dǎo)

【摘要】：消化道中定植有大量菌群,消化道上皮細(xì)胞與這些菌群直接接觸,是腸道粘膜免疫的第一道防線,其分泌的抗菌肽分子在維持腸道微生態(tài)平衡、抵抗病原菌感染中占有重要地位。人β-防御素-2(hBD-2)是一重要的抗菌肽分子,廣泛表達于皮膚、呼吸道、消化道、泌尿生殖道等粘膜上皮細(xì)胞中,具有重要的抗感染功能。hBD-2具有誘導(dǎo)表達的特點,通常情況下腸道上皮細(xì)胞不表達hBD-2,但在受到致病因子刺激時,hBD-2的表達量迅速提高,進而發(fā)揮粘膜保護功能。 以往的研究顯示病原菌如腸炎性沙門氏菌,侵襲性大腸桿菌等能夠誘導(dǎo)人腸道上皮細(xì)胞hBD-2表達,但是益生菌與抗菌肽之間的關(guān)系還沒有相關(guān)研究,而且hBD-2誘導(dǎo)表達的調(diào)控及其信號轉(zhuǎn)導(dǎo)機制十分復(fù)雜,目前尚不完全清楚。雙歧桿菌作為人體主要益生菌之一,參與了宿主的消化、營養(yǎng)、代謝、吸收、免疫及抗感染過程。因此通過探討雙歧桿菌與hBD-2表達之間的關(guān)系,尋找誘導(dǎo)hBD-2基因表達的有效刺激因子,不僅能夠通過提高粘膜局部hBD-2濃度而達到殺滅病原菌的目的,而且有助于在分子水平上揭示機體抗感染免疫防御機制。 本項研究目的是檢測雙歧桿菌對人腸腺上皮細(xì)胞hBD-2基因的激活作用并分離其活性組分,進一步探討其受體及其胞內(nèi)信號傳導(dǎo)通路。 首先,我們研究了雙歧桿菌對人腸腺上皮細(xì)胞hBD-2基因的激活作用并分離其活性組分。厭氧培養(yǎng)長雙歧桿菌,采用超聲破碎細(xì)胞、超速離心、蛋白萃取方法獲得雙歧桿菌胞壁蛋白質(zhì)成份,分別利用活菌,熱滅活全菌,全
[Abstract]:There are a lot of colonization in the digestive tract microflora, digestive tract epithelial cells and these bacteria in direct contact, is the first line of defense of intestinal mucosal immunity, the secretion of antimicrobial peptides on intestinal micro ecological balance, resistance plays an important role in pathogen infection. Human beta defensin -2 (hBD-2) is one of the most important antibacterial peptide, is widely expressed in skin, respiratory, gastrointestinal, urogenital tract and other mucosal epithelial cells, has an important function of.HBD-2 has the characteristics of anti infection induced the expression of intestinal epithelial cells normally do not express hBD-2, but by the pathogenic factors of stimulation, the expression of hBD-2 increased rapidly, and then play the mucosa the protection function.
Previous studies have shown that the pathogens such as Salmonella enteritis, to invasive Escherichia coli hBD-2 induced by human intestinal epithelial cell expression, but the relationship between probiotics and antimicrobial peptides has not been studied, and the regulation of hBD-2 induced expression and signal transduction mechanism of ten complex, is not totally clear. As one of Bifidobacterium the main body involved in host probiotics, digestion, nutrition, metabolism, absorption, immunity and anti infection. Therefore, through discussing the relationship between the expression of hBD-2 and Bacillus bifidus, find effective stimulation factor induced hBD-2 gene expression, not only can to kill pathogens by improving mucosal hBD-2 concentration, but also help to reveal the body at the molecular level of anti infection immune defense mechanism.
The purpose of this study is to detect the activation of Bifidobacterium on hBD-2 gene of human intestinal epithelial cells and isolate their active components, and further explore their receptors and intracellular signaling pathways.
First, we studied the activation effect of Bifidobacterium on human intestinal epithelial cells hBD-2 gene and separation of active components. Anaerobic culture of Bifidobacterium longum, using ultrasonic cell, ultracentrifugation, protein extraction method of bifidobacterium cell wall protein content respectively by using heat inactivated bacteria, total bacteria, total

【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R37

【引證文獻】

相關(guān)碩士學(xué)位論文 前2條

1 張敏;HMGN2分子的分離純化及其抗乙型肝炎病毒活性研究[D];四川大學(xué);2007年

2 趙文斌;梓醇、左旋紫草素、丹皮酚調(diào)控角質(zhì)形成細(xì)胞人β防御素-2的機理研究[D];北京中醫(yī)藥大學(xué);2013年

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本文編號：1362179