目的:近年來(lái)發(fā)現(xiàn)內(nèi)皮抑素可以抑制組織纖維化,但是具體機(jī)制不詳。本文旨在研究?jī)?nèi)皮抑素抑制纖維化的作用機(jī)制。創(chuàng)新點(diǎn):首次闡明了內(nèi)皮抑素對(duì)成纖維細(xì)胞PDGFRβ（血小板衍生生長(zhǎng)因子受體β）/ERK（細(xì)胞外調(diào)節(jié)蛋白激酶）信號(hào)通路的影響及其與纖維化的關(guān)系。方法:體外培養(yǎng)人成纖維細(xì)胞,采用血小板衍生生長(zhǎng)因子-BB（PDGF-BB）或轉(zhuǎn)化生長(zhǎng)因子-β1（TGF-β1）建立細(xì)胞纖維化模型,進(jìn)一步應(yīng)用內(nèi)皮抑素處理,通過(guò)檢測(cè)膠原及肌成纖維細(xì)胞表面標(biāo)志等了解內(nèi)皮抑素對(duì)細(xì)胞纖維化和表型轉(zhuǎn)化的抑制作用。進(jìn)而檢測(cè)內(nèi)皮抑素對(duì)成纖維細(xì)胞PDGFRβ/ERK信號(hào)通路的影響,并分析其可能與抗纖維化作用存在的關(guān)聯(lián)。結(jié)論:內(nèi)皮抑素通過(guò)調(diào)控PDGFRβ/ERK信號(hào)通路抑制瘢痕增生,是多靶點(diǎn)的、具有較好的抗纖維化臨床應(yīng)用前景的藥物。 

【文章頁(yè)數(shù)】：8 頁(yè)

【文章目錄】：
1 Introduction
2 Materials and methods
    2.1 Reagents and antibodies
    2.2 Culture of human skin fibroblasts
    2.3 Treatments and groups
    2.4 MTT cytotoxicity assay
    2.5 ELISA assay
    2.6 Western blot analysis
    2.7 Immunofluorescent staining
    2.8 Statistical analysis
3 Results
    3.1 Toxicity of endostatin with MTT cytotoxicity assay
    3.2 Effect of endostatin on fibrosis
    3.3 Inhibitive effect of endostatin on the expres-sion of PDGFR, p-PDGFR, and p-ERK
4 Discussion
5 Conclusions
Compliance with ethics guidelines


【參考文獻(xiàn)】：
期刊論文
[1]Endostatin inhibits hypertrophic scarring in a rabbit ear model[J]. Hai-tao REN,Hang HU,Yuan LI,Hong-fei JIANG,Xin-lei HU,Chun-mao HAN.  Journal of Zhejiang University-Science B（Biomedicine ＆ Biotechnology）. 2013(03)



本文編號(hào)：3731432