【摘要】：目的研究青海結核分枝桿菌對4種一線抗結核藥物耐藥狀況及與Spoligotyping基因型的關系,為結核病有效預防提供依據(jù)。方法采用常規(guī)比例法對分離的251株結核分枝桿菌進行異煙肼(INH)、利福平(RFP)、鏈霉素(SM)和乙胺丁醇(EMB)等4種一線抗結核藥物的藥敏試驗,并對菌株進行Spoligotyping分型,對藥敏試驗和基因分型結果進行綜合分析。結果 251株結核分枝桿菌中,總耐藥率為56.2%;對INH、RFP、SM和EMB的耐藥率分別為43.0%(108/251)、37.1%(93/251)、39.0%(98/251)和27.9%(70/251);耐多藥率為31.5%(79/251)。所有菌株經(jīng)Spoligotyping分型,分為北京基因型185株(73.7%)與非北京型菌株66株(26.3%)。未發(fā)現(xiàn)北京型與非北京型結核菌株與耐藥存在統(tǒng)計學關聯(lián)。結論青海流行的結核分枝桿菌耐藥率及多耐藥率較高,Spoligotyping分型顯示北京基因型為主要流行型。
[Abstract]:Objective to study the resistance of Mycobacterium tuberculosis to four first-line antituberculosis drugs and its relationship with Spoligotyping genotype, so as to provide evidence for effective prevention of tuberculosis. Methods the drug sensitivity test of isoniazid (INH), rifampicin (RFP), streptomycin (SM) and ethambutol (EMB) was carried out on 251 strains of Mycobacterium tuberculosis by conventional proportional method. The strains were typed by Spoligotyping, and the results of drug sensitivity test and genotyping were analyzed comprehensively. Results among 251 strains of Mycobacterium tuberculosis, the total drug resistance rate was 56.2%, the resistance rate to INH,RFP,SM and EMB was 43.0% (108 鈮，

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