發(fā)布時(shí)間：2019-06-26 16:13

【摘要】：目的探尋一種滿意的芽孢染色方法 ,同時(shí)該方法能克服染料加溫后揮發(fā)給實(shí)驗(yàn)操作人員帶來的身體傷害。方法以碘液為媒染劑,用冷染方法替代傳統(tǒng)芽孢加溫染色,然后進(jìn)行細(xì)菌涂片、干燥、固定、染色。初染液為石炭酸復(fù)紅5 min,碘液媒染1 min,95%乙醇脫色5 s,堿性美蘭復(fù)染1 min。比較石炭酸復(fù)紅染色后加碘液、石炭酸復(fù)紅染色不加碘液、石炭酸復(fù)紅加溫染色不加碘液的染色效果。結(jié)果石炭酸復(fù)紅染色后加碘液與石炭酸復(fù)紅加溫染色不加碘液的染色效果相同,石炭酸復(fù)紅染色不加碘液也能看出芽孢,但不明顯。結(jié)論用碘液助染可獲得滿意的芽孢染色結(jié)果,且用該法可減少石炭酸揮發(fā)給實(shí)驗(yàn)操作人員帶來的危害。
[Abstract]:Objective to explore a satisfactory method of spore staining, which can overcome the physical injury caused by the volatilization of dye after heating. Methods iodine solution was used as mordant, cold staining method was used instead of traditional spore heating staining, and then bacterial smear, drying, fixation and staining were carried out. The initial dye was decolorized by 1 min,95% ethanol and 1 min. by carbonic acid red 5 min, iodine solution mordant staining for 5 s and alkaline Meilan restaining for 1 min.. The dyeing effects of iodized solution, carbonic acid red staining without iodine solution and carbonic acid rered staining without iodine solution were compared. Results the staining effect of adding iodine solution was the same as that of carbonic acid rered staining without iodine solution. Spores could also be seen by carbonic acid red staining without iodine solution, but it was not obvious. Conclusion satisfactory spore staining results can be obtained with iodine solution, and the harm caused by carbonic acid volatilization to experimental operators can be reduced by this method.
【作者單位】： 第三軍醫(yī)大學(xué)微生物學(xué)教研室;第三軍醫(yī)大學(xué)新橋醫(yī)院感控科;第三軍醫(yī)大學(xué)免疫學(xué)教研室;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(31570173)
【分類號】：R361
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本文編號：2506309