以氣味作為相關(guān)環(huán)境線索的大鼠條件性位置偏好實(shí)驗(yàn)
發(fā)布時(shí)間：2019-05-28 08:23
【摘要】：實(shí)驗(yàn)?zāi)康模涸跅l件性位置偏好實(shí)驗(yàn)中,為了最大程度區(qū)分兩種環(huán)境,實(shí)驗(yàn)一般采用多線索條件刺激。但多線索條件刺激的注意之處是：環(huán)境刺激單獨(dú)與非條件刺激形成的聯(lián)系還是各種刺激組合成的實(shí)驗(yàn)情境與藥物的獎(jiǎng)賞作用形成的聯(lián)系。因此,單一線索的條件性位置偏好實(shí)驗(yàn)可能有利于某些實(shí)驗(yàn)研究。本文以單一線索--氣味作為相關(guān)環(huán)境線索進(jìn)行條件性位置偏好實(shí)驗(yàn),分析氣味作為單一線索在大鼠嗎啡條件性位置偏好實(shí)驗(yàn)中的作用。 實(shí)驗(yàn)方法：成年雄性SD大鼠,隨機(jī)分為對應(yīng)氣味組、相反氣味組和無氣味組等三組,每組隨機(jī)分為藥物組(嗎啡組)和對照組(生理鹽水組)。實(shí)驗(yàn)系統(tǒng)由位置偏好行為測試箱和視頻記錄檢測系統(tǒng)組成。位置偏好行為箱由兩個(gè)大小相同的實(shí)驗(yàn)箱和一個(gè)中間過渡箱組成。兩個(gè)實(shí)驗(yàn)箱的視覺和觸覺均相同,兩端分別放置滴有玫瑰原液或薄荷原液的小瓶。視頻記錄系統(tǒng)由安放在測試箱上方的攝像頭和計(jì)算機(jī)軟件系統(tǒng)組成,大鼠的行為數(shù)據(jù)由計(jì)算機(jī)軟件記錄和分析。實(shí)驗(yàn)分為三個(gè)階段。第一階段：適應(yīng)階段,即條件化前期。第1-3天,每天讓大鼠在行為測試箱中自由活動15分鐘(900秒),錄像全過程,然后將大鼠分組放入各自房間3小時(shí),每組的房間環(huán)境完全相同。第二階段,條件化建立期。第4-11天,共8天。在玫瑰與薄荷中隨機(jī)選擇一種氣味為陽性氣味,對應(yīng)的實(shí)驗(yàn)箱為陽性氣味箱。每天給嗎啡組大鼠腹腔注射嗎啡(10mg/kg)或者同等劑量的生理鹽水,放入實(shí)驗(yàn)箱45分鐘。然后放入各自房間3小時(shí),對應(yīng)氣味組的房間內(nèi)給予當(dāng)天在實(shí)驗(yàn)箱中相同氣味；相反氣味組的房間內(nèi)給予與當(dāng)天在實(shí)驗(yàn)箱中氣味不同的另一種氣味；無氣味組則每天房間內(nèi)不給予氣味。生理鹽水組大鼠每天注射等量生理鹽水,其余操作與嗎啡組相同。第三階段,檢測階段。每天讓大鼠在行為箱中自由活動15分鐘,錄像全過程。分析大鼠在兩個(gè)實(shí)驗(yàn)箱中停留的時(shí)間。 數(shù)據(jù)分析方法：大鼠在CPP陽性氣味箱停留時(shí)間以秒(s)為記錄單位,采用SPSS11.5軟件為統(tǒng)計(jì)工具進(jìn)行數(shù)據(jù)分析。采用單因素方差分析和重復(fù)測量方差分析, P0.05為有統(tǒng)計(jì)學(xué)差異。 實(shí)驗(yàn)結(jié)果：條件化前期,采用單因素方差分析,各組大鼠在CPP陽性氣味箱停留時(shí)間沒有統(tǒng)計(jì)學(xué)差異(p0.05)；檢測階段,采用單因素方差分析和重復(fù)測量方差分析,藥物處理與氣味無交互效應(yīng)(p＞0.05),各組大鼠在陽性氣味箱停留時(shí)間沒有統(tǒng)計(jì)學(xué)差異(p0.05)。 結(jié)論：氣味作為單一線索未能建立大鼠嗎啡條件性位置偏好,可能與氣味具有揮發(fā)性,濃度難以控制等有關(guān)。
[Abstract]:Objective: in order to distinguish the two environments to the greatest extent, multi-clue conditional stimulation is usually used in conditional position preference experiment. However, the attention of multi-clue conditional stimulation is the relationship between environmental stimulation alone and non-conditional stimulation or the relationship between the experimental situation formed by the combination of various stimuli and the reward effect of drugs. Therefore, the conditional position preference experiment of a single clue may be beneficial to some experimental studies. In this paper, the conditioned position preference experiment was carried out with a single clue, odour as a related environmental clue, and the role of odor as a single clue in morphine conditioned position preference experiment in rats was analyzed. Methods: adult male SD rats were randomly divided into three groups: corresponding odor group, odor group and odorless group. Each group was randomly divided into drug group (morphine group) and control group (saline group). The experimental system is composed of position preference behavior test box and video recording detection system. The position preference behavior box consists of two experimental boxes of the same size and an intermediate transition box. The visual and tactile senses of the two experimental boxes were the same, with bottles dripped with rose solution or mint solution at both ends. The video recording system is composed of a camera and a computer software system placed above the test box, and the behavior data of rats are recorded and analyzed by computer software. The experiment is divided into three stages. The first stage: adaptation stage, that is, the early stage of conditionalization. On the 1st-3rd day, the rats were allowed to move freely in the behavior test box for 15 minutes (900 seconds) every day, and then the rats were grouped into their respective rooms for 3 hours, and the room environment of each group was exactly the same. The second stage, the conditional establishment period. 4-11 days, a total of 8 days. In rose and mint, a kind of smell was randomly selected as positive smell, and the corresponding experimental box was positive smell box. Morphine (10mg/kg) or the same dose of saline were injected intraperitoneally into morphine group every day and put in the experimental box for 45 minutes. Then put in their respective rooms for 3 hours, the room of the corresponding odor group was given the same smell in the experimental box on the same day, on the contrary, the room of the odor group was given another smell which was different from that in the experimental box on the same day. The odour free group did not give odour in the room every day. The rats in saline group were injected with the same amount of saline every day, and the other operations were the same as those in morphine group. The third stage, the detection stage. The rats were allowed to move freely in the behavior box for 15 minutes every day, and the whole process was videotaped. The time of rats staying in two experimental boxes was analyzed. Data analysis methods: the residence time of rats in CPP positive flavor box was recorded in second (s), and SPSS11.5 software was used as statistical tool to analyze the data. Using single factor variance analysis and repeated measurement variance analysis, P 0.05 was statistically significant. The results showed that there was no significant difference in the residence time of CPP positive flavor box in each group by single factor variance analysis in the early stage of conditionalization (p0.05). In the detection stage, single factor variance analysis and repeated measurement variance analysis were used. There was no interaction effect between drug treatment and smell (p > 0.05). There was no significant difference in the residence time of rats in positive flavor box (p0.05). Conclusion: odour as a single clue can not establish morphine conditioned position preference in rats, which may be related to odour volatility and difficult concentration control.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R-332
[Abstract]:Objective: in order to distinguish the two environments to the greatest extent, multi-clue conditional stimulation is usually used in conditional position preference experiment. However, the attention of multi-clue conditional stimulation is the relationship between environmental stimulation alone and non-conditional stimulation or the relationship between the experimental situation formed by the combination of various stimuli and the reward effect of drugs. Therefore, the conditional position preference experiment of a single clue may be beneficial to some experimental studies. In this paper, the conditioned position preference experiment was carried out with a single clue, odour as a related environmental clue, and the role of odor as a single clue in morphine conditioned position preference experiment in rats was analyzed. Methods: adult male SD rats were randomly divided into three groups: corresponding odor group, odor group and odorless group. Each group was randomly divided into drug group (morphine group) and control group (saline group). The experimental system is composed of position preference behavior test box and video recording detection system. The position preference behavior box consists of two experimental boxes of the same size and an intermediate transition box. The visual and tactile senses of the two experimental boxes were the same, with bottles dripped with rose solution or mint solution at both ends. The video recording system is composed of a camera and a computer software system placed above the test box, and the behavior data of rats are recorded and analyzed by computer software. The experiment is divided into three stages. The first stage: adaptation stage, that is, the early stage of conditionalization. On the 1st-3rd day, the rats were allowed to move freely in the behavior test box for 15 minutes (900 seconds) every day, and then the rats were grouped into their respective rooms for 3 hours, and the room environment of each group was exactly the same. The second stage, the conditional establishment period. 4-11 days, a total of 8 days. In rose and mint, a kind of smell was randomly selected as positive smell, and the corresponding experimental box was positive smell box. Morphine (10mg/kg) or the same dose of saline were injected intraperitoneally into morphine group every day and put in the experimental box for 45 minutes. Then put in their respective rooms for 3 hours, the room of the corresponding odor group was given the same smell in the experimental box on the same day, on the contrary, the room of the odor group was given another smell which was different from that in the experimental box on the same day. The odour free group did not give odour in the room every day. The rats in saline group were injected with the same amount of saline every day, and the other operations were the same as those in morphine group. The third stage, the detection stage. The rats were allowed to move freely in the behavior box for 15 minutes every day, and the whole process was videotaped. The time of rats staying in two experimental boxes was analyzed. Data analysis methods: the residence time of rats in CPP positive flavor box was recorded in second (s), and SPSS11.5 software was used as statistical tool to analyze the data. Using single factor variance analysis and repeated measurement variance analysis, P 0.05 was statistically significant. The results showed that there was no significant difference in the residence time of CPP positive flavor box in each group by single factor variance analysis in the early stage of conditionalization (p0.05). In the detection stage, single factor variance analysis and repeated measurement variance analysis were used. There was no interaction effect between drug treatment and smell (p > 0.05). There was no significant difference in the residence time of rats in positive flavor box (p0.05). Conclusion: odour as a single clue can not establish morphine conditioned position preference in rats, which may be related to odour volatility and difficult concentration control.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R-332
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