【摘要】：目的探討含非甲基化胞嘧啶和鳥嘌呤核苷酸序列的寡脫氧核糖核苷酸(CpG-ODN)對卡介苗(BCG)的免疫增強(qiáng)作用,為提高BCG的免疫效果提供實(shí)驗(yàn)依據(jù)。方法 (1)細(xì)胞實(shí)驗(yàn):分空白組、低濃度組和高濃度組,均接種小鼠巨噬細(xì)胞株Ana-1預(yù)培養(yǎng)。分別加入培養(yǎng)液、低濃度CpG-ODN和高濃度CpG-ODN,培養(yǎng)后測定上清液中IL-12、IL-18和IFN-γ含量。(2)動(dòng)物實(shí)驗(yàn):分對照組(A組)、感染組(B組)、BCG預(yù)防組(C組)和BCG+CpG-ODN預(yù)防組(D組)。A組常規(guī)飼養(yǎng),B、C、D組分別注射無菌PBS、BCG、BCG和CpG-ODN混合液,2周后B、C、D組注射結(jié)核分枝桿菌,再2周后處死所有小鼠,取肺組織病檢,收集血清測定IL-12、IL-18和IFN-γ含量。結(jié)果 (1)細(xì)胞實(shí)驗(yàn):CpG-ODN刺激培養(yǎng)下,Ana-1分泌IL-12、IL-18和IFN-γ均呈CpG-ODN劑量依賴性,加入CpG-ODN含量越高,Ana-1分泌上述三種Th1型細(xì)胞因子含量越多。(2)動(dòng)物實(shí)驗(yàn):A組肺組織未見炎癥細(xì)胞浸潤;B組肺組織可見大量炎癥細(xì)胞浸潤;C組和D組肺組織可見少量炎癥細(xì)胞浸潤。B組小鼠血清中IL-12、IL-18和IFN-γ含量均顯著低于A組小鼠(均P0.05)。C組和D組小鼠血清中IL-12、IL-18和IFN-γ含量均顯著高于A組(均P0.05)。D組小鼠血清中IL-12、IL-18和IFN-γ含量均顯著高于C組(均P0.05)。結(jié)論 CpG-ODN能夠增強(qiáng)BCG的免疫預(yù)防效果,該免疫增強(qiáng)作用可能與CpG-ODN上調(diào)Th1型細(xì)胞因子含量有關(guān)。
[Abstract]:Aim to investigate the immunoenhancement effect of oligodeoxyribonucleotide (CpG-ODN) containing unmethylated cytosine and guanine nucleotide sequence on BCG (BCG) in order to provide experimental basis for improving the immune effect of BCG. Methods (1) Cell experiment: divided into three groups: blank group, low concentration group and high concentration group, all of them were inoculated with mouse macrophage cell line Ana-1 pre-culture. The contents of IL-12,IL-18 and IFN- 緯 in the supernatant were determined after culture with low concentration of CpG-ODN and high concentration of CpG-ODN, respectively. (2) Animal experiment: divided into control group (group A), infection group (group B), control group (group A) and infection group (group B). BCG prevention group (group C) and BCG CpG-ODN prevention group (group D,). A, B, C, D) were injected with aseptic PBS,BCG,BCG and CpG-ODN mixture respectively. Two weeks later, Mycobacterium tuberculosis was injected into group B, C and D, respectively. After 2 weeks, all the mice were killed for lung pathological examination and serum levels of IL-12,IL-18 and IFN- 緯 were determined. Results (1) Cell experiment: under the stimulation of CpG-ODN, Ana-1 secreted IL-12,IL-18 and IFN- 緯 in a dose-dependent manner, and the higher the content of CpG-ODN, the higher the content of CpG-ODN. Ana-1 secreted more Th1 cytokines. (2) Animal experiment: there was no infiltration of inflammatory cells in lung tissue in group A; In group B, a large number of inflammatory cell infiltration was observed in lung tissue. The levels of IL-12,IL-18 and IFN- 緯 in serum of mice in group B were significantly lower than those in group A (P0.05,). C and D, P 0.05), and the levels of IL-12, in serum of mice in group B were significantly lower than those in group A (P 0.05). The levels of IL-18 and IFN- 緯 in serum of group A were significantly higher than those of group A (P0.05). The levels of IL-12,IL-18 and IFN- 緯 in serum of). D group were significantly higher than those of group C (P0.05). Conclusion CpG-ODN can enhance the immunoprophylactic effect of BCG, which may be related to the up-regulation of Th1 cytokine content by CpG-ODN.
【作者單位】： 深圳市羅湖區(qū)慢性病防治院;深圳市羅湖區(qū)婦幼保健院;
【基金】：深圳市羅湖區(qū)科技計(jì)劃項(xiàng)目(編號:2014040)
【分類號】：R392

【相似文獻(xiàn)】

相關(guān)期刊論文 前8條

1 陳暉;林海英;孟春;郭養(yǎng)浩;;細(xì)菌DNA免疫增強(qiáng)作用的研究[J];藥物生物技術(shù);2006年01期

2 方福德;小球藻來源的食品添加劑對接種流感疫苗的健康成人的免疫增強(qiáng)作用和安全性[J];中華預(yù)防醫(yī)學(xué)雜志;2004年01期

3 劉燕瑜;任靜強(qiáng);張丹;譚磊;劉存霞;靖杰;劉昊;魯會(huì)軍;金擴(kuò)世;金寧一;;Quil A對O型口蹄疫疫苗的免疫增強(qiáng)作用[J];中國生物制品學(xué)雜志;2011年08期

4 匡彥德,王美英,錢琴芳,謝家駿,曹蓓莉,關(guān)惠貞,吳耀晴;冬蟲夏草免疫增強(qiáng)作用機(jī)理的研究[J];上海免疫學(xué)雜志;1989年01期

5 田瑞陽,韓威,俞纓,陳悅,虞谷松,楊勝利,龔毅;人B淋巴細(xì)胞刺激因子C端肽的免疫增強(qiáng)作用[J];生物化學(xué)與生物物理學(xué)報(bào);2003年12期

6 曹錫坤;;抗Ia和抗K、D抗體的免疫增強(qiáng)作用[J];國外醫(yī)學(xué)(免疫學(xué)分冊);1981年03期

7 藍(lán)景剛,胡宏;雙歧桿菌及其表面分子的免疫增強(qiáng)作用[J];中國微生態(tài)學(xué)雜志;1999年03期

8 田明禮,易新元,曾憲芳,彭先楚,曾慶仁,Larry McReynolds;佐劑FCA與Quil A對rGST-Sj32的免疫增強(qiáng)作用的比較[J];中國寄生蟲病防治雜志;2001年03期

相關(guān)碩士學(xué)位論文 前2條

1 張金萍;CpG-ODN免疫增強(qiáng)作用的實(shí)驗(yàn)研究[D];暨南大學(xué);2003年

2 鄭梅珍;CpG特征序列的篩選及CpG復(fù)合物免疫增強(qiáng)作用的研究[D];暨南大學(xué);2004年

，

本文編號：2470539