小鼠胚胎呼吸內(nèi)胚層相關(guān)第二生心區(qū)的發(fā)育
發(fā)布時(shí)間：2019-04-08 18:37
【摘要】：目的探討小鼠胚胎心流出道分隔過(guò)程中,前腸呼吸內(nèi)胚層與咽前第二生心區(qū)細(xì)胞發(fā)育的形態(tài)學(xué)關(guān)系及機(jī)制。方法胚齡9~13d小鼠胚胎標(biāo)本各6例,連續(xù)石蠟切片,用抗轉(zhuǎn)錄因子叉頭框蛋白A2(Foxa2)、抗胰島因子1(ISL-1)、抗patched1(Ptc1)、抗patched 2(Ptc2)、抗α-平滑肌肌動(dòng)蛋白(α-SMA)及抗心肌肌球蛋白重鏈(MHC)抗體進(jìn)行免疫組織化學(xué)及免疫熒光染色。結(jié)果胚齡9~9.5d,前腸腹側(cè)壁ISL-1陽(yáng)性內(nèi)胚層局部增厚,呼吸內(nèi)胚層開(kāi)始發(fā)育,ISL-1陽(yáng)性間充質(zhì)細(xì)胞緊隨其后開(kāi)始出現(xiàn)在呼吸內(nèi)胚層周圍的基質(zhì)中。胚齡10~11.5d,呼吸內(nèi)胚層向動(dòng)脈囊方向生長(zhǎng)延伸向喉-氣管溝演變,ISL-1陽(yáng)性咽前間充質(zhì)細(xì)胞圍繞呼吸內(nèi)胚層呈對(duì)稱的特征性錐體形結(jié)構(gòu)分布,錐體頂端突入動(dòng)脈囊腔向主-肺動(dòng)脈隔發(fā)育。在喉-氣管溝發(fā)育過(guò)程中,總能觀察到1條實(shí)心內(nèi)胚層細(xì)胞索位于其腹側(cè)頂端,Ptc1和Ptc2主要局限于發(fā)育中的喉-氣管溝及實(shí)心細(xì)胞索表達(dá),喉-氣管溝及實(shí)心細(xì)胞索的內(nèi)胚層則位于錐體結(jié)構(gòu)的中心。胚齡12~13d,在流出道水平前腸分隔形成氣管,內(nèi)胚層細(xì)胞索逐漸消失,氣管上皮逐漸失去Ptc1和Ptc2表達(dá),氣管腹側(cè)的ISL-1陽(yáng)性間充質(zhì)細(xì)胞密度明顯減低,并逐漸停止向流出道添加,動(dòng)脈囊分隔完成。結(jié)論呼吸內(nèi)胚層的分化發(fā)育與咽前ISL-1陽(yáng)性第二生心區(qū)細(xì)胞的發(fā)育聚集密切耦聯(lián)。音猬因子(SHH)信號(hào)系統(tǒng)在呼吸內(nèi)胚層發(fā)育過(guò)程中活躍程度較高,發(fā)育中的呼吸內(nèi)胚層可能作為組織中心,通過(guò)SHH信號(hào)通路誘導(dǎo)ISL-1陽(yáng)性細(xì)胞的聚集,并通過(guò)內(nèi)胚層生長(zhǎng)延伸造成的機(jī)械牽拉力驅(qū)動(dòng)ISL-1陽(yáng)性細(xì)胞遷移,參與流出道正常形態(tài)發(fā)生。
[Abstract]:Aim to investigate the morphological relationship and mechanism between the endoderm of pregut respiration and the cell development of prepharyngeal second heart during the separation of cardiac outflow tract in mouse embryos. Methods six embryonic specimens of 9-day-old 13-day-old mice were randomly divided into two groups: paraffin section, anti-Foxa2, anti-islet factor-1 (ISL-1), anti-patched1 (Ptc1) and anti-patched-2 (Ptc2). Anti-偽-smooth muscle actin (偽-SMA) and anti-myosin heavy chain (MHC) antibodies were stained by immunohistochemistry and immunofluorescence. Results ISL-1-positive endoderm was thickened in ventral wall of anterior intestine at 9-9. 5 days, respiratory endoderm began to develop, and ISL-1-positive mesenchymal cells began to appear in the matrix around respiratory endoderm. At the age of 10 to 11.5 days, the respiratory endoderm grew and extended toward the larynx-trachea sulcus, and the ISL-1-positive prepharyngeal mesenchymal cells showed symmetrical, characteristic cone-shaped distribution around the respiratory endoderm. The capsular cavity of the artery protruding into the apex of the pyramidal developed into the aortic pulmonary septum. During the development of larynx-trachea sulcus, a solid endoderm cell cord was always observed at its ventral tip. The expression of Ptc1 and Ptc2 was mainly confined to the developing laryngeal-tracheal sulcus and the solid cell cord during the development of larynx-tracheobronchial sulcus. The endoderm of larynx-trachea sulcus and solid cell cord is located in the center of pyramidal structure. At the age of 12 ~ 13 days, trachea was separated from the anterior intestine at the outflow tract level, the endoderm cell cord disappeared gradually, the expression of Ptc1 and Ptc2 was gradually lost in the trachea epithelium, and the density of ISL-1 positive mesenchymal cells in the ventral trachea was significantly decreased. And gradually stop adding to the outflow tract, arterial sac separation is complete. Conclusion the differentiation and development of respiratory endoderm are closely coupled with the development and aggregation of prepharyngeal ISL-1-positive secondary heart cells. The phonic hedgehog factor (SHH) signaling system is highly active in the development of respiratory endoderm. The developing respiratory endoderm may act as a tissue center and induce the aggregation of ISL-1 positive cells through SHH signaling pathway. The migration of ISL-1-positive cells was driven by mechanical pulling force caused by endoderm growth and extension, which was involved in the normal morphogenesis of outflow tract.
【作者單位】： 山西醫(yī)科大學(xué)組織學(xué)與胚胎學(xué)教研室;長(zhǎng)春醫(yī)學(xué)高等專科學(xué)校解剖學(xué)教研室;山西醫(yī)學(xué)科學(xué)院神經(jīng)內(nèi)科;
【基金】：國(guó)家自然科學(xué)基金(30771141,31200899) 山西省自然科學(xué)基金(2015021189) 山西省優(yōu)勢(shì)重點(diǎn)學(xué)科建設(shè)經(jīng)費(fèi)(轉(zhuǎn)化醫(yī)學(xué)) 山西醫(yī)科大學(xué)博士啟動(dòng)基金(03201410);山西醫(yī)科大學(xué)331基礎(chǔ)醫(yī)學(xué)科技培植基金(201423)
【分類號(hào)】：R321.5
本文編號(hào)：2454814
[Abstract]:Aim to investigate the morphological relationship and mechanism between the endoderm of pregut respiration and the cell development of prepharyngeal second heart during the separation of cardiac outflow tract in mouse embryos. Methods six embryonic specimens of 9-day-old 13-day-old mice were randomly divided into two groups: paraffin section, anti-Foxa2, anti-islet factor-1 (ISL-1), anti-patched1 (Ptc1) and anti-patched-2 (Ptc2). Anti-偽-smooth muscle actin (偽-SMA) and anti-myosin heavy chain (MHC) antibodies were stained by immunohistochemistry and immunofluorescence. Results ISL-1-positive endoderm was thickened in ventral wall of anterior intestine at 9-9. 5 days, respiratory endoderm began to develop, and ISL-1-positive mesenchymal cells began to appear in the matrix around respiratory endoderm. At the age of 10 to 11.5 days, the respiratory endoderm grew and extended toward the larynx-trachea sulcus, and the ISL-1-positive prepharyngeal mesenchymal cells showed symmetrical, characteristic cone-shaped distribution around the respiratory endoderm. The capsular cavity of the artery protruding into the apex of the pyramidal developed into the aortic pulmonary septum. During the development of larynx-trachea sulcus, a solid endoderm cell cord was always observed at its ventral tip. The expression of Ptc1 and Ptc2 was mainly confined to the developing laryngeal-tracheal sulcus and the solid cell cord during the development of larynx-tracheobronchial sulcus. The endoderm of larynx-trachea sulcus and solid cell cord is located in the center of pyramidal structure. At the age of 12 ~ 13 days, trachea was separated from the anterior intestine at the outflow tract level, the endoderm cell cord disappeared gradually, the expression of Ptc1 and Ptc2 was gradually lost in the trachea epithelium, and the density of ISL-1 positive mesenchymal cells in the ventral trachea was significantly decreased. And gradually stop adding to the outflow tract, arterial sac separation is complete. Conclusion the differentiation and development of respiratory endoderm are closely coupled with the development and aggregation of prepharyngeal ISL-1-positive secondary heart cells. The phonic hedgehog factor (SHH) signaling system is highly active in the development of respiratory endoderm. The developing respiratory endoderm may act as a tissue center and induce the aggregation of ISL-1 positive cells through SHH signaling pathway. The migration of ISL-1-positive cells was driven by mechanical pulling force caused by endoderm growth and extension, which was involved in the normal morphogenesis of outflow tract.
【作者單位】： 山西醫(yī)科大學(xué)組織學(xué)與胚胎學(xué)教研室;長(zhǎng)春醫(yī)學(xué)高等專科學(xué)校解剖學(xué)教研室;山西醫(yī)學(xué)科學(xué)院神經(jīng)內(nèi)科;
【基金】：國(guó)家自然科學(xué)基金(30771141,31200899) 山西省自然科學(xué)基金(2015021189) 山西省優(yōu)勢(shì)重點(diǎn)學(xué)科建設(shè)經(jīng)費(fèi)(轉(zhuǎn)化醫(yī)學(xué)) 山西醫(yī)科大學(xué)博士啟動(dòng)基金(03201410);山西醫(yī)科大學(xué)331基礎(chǔ)醫(yī)學(xué)科技培植基金(201423)
【分類號(hào)】：R321.5
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