【摘要】：羊駝體內(nèi)存在天然缺失輕鏈的重鏈抗體(HcAb),其單域抗原結(jié)合片段也稱為VHH或納米抗體(nanobody,Nb),是目前已知的最小抗原結(jié)合片段。與傳統(tǒng)抗體相比,納米抗體具有分子量小(12~15kDa)、穩(wěn)定性好和免疫原性低等特點(diǎn),這些特點(diǎn)使得VHH在基礎(chǔ)研究、診斷及治療上具有極大的應(yīng)用價(jià)值,目前已有多種納米抗體進(jìn)入了臨床研究階段。綜述了近年來VHH在革蘭氏陰性菌(大腸桿菌)、革蘭氏陽性菌(芽孢桿菌和乳酸桿菌)、酵母、絲狀真菌、昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞和植物細(xì)胞中異源表達(dá)的研究現(xiàn)狀,包括表達(dá)系統(tǒng)、宿主、載體特點(diǎn)、載體構(gòu)建方式及產(chǎn)量等;從分子水平、表達(dá)水平和理性設(shè)計(jì)三個(gè)層面探討了納米抗體產(chǎn)量提高的策略,以期為納米抗體研究者提供借鑒和思路。
[Abstract]:There is a heavy chain antibody (HcAb), with natural deletion of light chain in alpaca, and its single domain antigen binding fragment is also called VHH or nano antibody (nanobody,Nb), which is the smallest antigen binding fragment known at present. Compared with traditional antibodies, nano-antibodies have the characteristics of small molecular weight (12~15kDa), good stability and low immunogenicity. These characteristics make VHH have great application value in basic research, diagnosis and treatment. At present, many kinds of nano-antibodies have entered the clinical research stage. This paper reviews the research status of heterologous expression of VHH in Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Bacillus and Lactobacillus), yeast, filamentous fungi, insect cells, mammalian cells and plant cells in recent years. Including expression system, host, vector characteristics, vector construction method and yield, etc. From the molecular level, expression level and rational design, the strategy to improve the production of nano-antibody was discussed in order to provide references and ideas for nano-antibody researchers.
【作者單位】： 天津大學(xué)化工學(xué)院系統(tǒng)生物工程教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(31470967)
【分類號】：R392

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