【摘要】：目的：研究布比卡因?qū)Ω栺R林炎性疼痛模型中脊髓膠質(zhì)細胞及炎性因子的影響。 方法：成年雄性SD大鼠，體重200-220克，取大鼠72只，隨機分為4組,假手術(shù)組：右足底掌部皮下注射0.1mL生理鹽水；炎性痛組：右足底掌部皮下注射2.5%福爾馬林試劑0.1mL，建立福爾馬林炎性痛模型；布比卡因組：經(jīng)蛛網(wǎng)膜下腔注射0.125%布比卡因40μL后同炎性痛組建立炎性疼痛模型；生理鹽水組：經(jīng)蛛網(wǎng)膜下腔注射40μL生理鹽水后同炎性痛組建立炎性疼痛模型。于建立右足底掌部炎性疼痛模型的術(shù)前、術(shù)后1d、術(shù)后3d和術(shù)后5d分別觀察機械縮足反射閾值（MWT）及累積疼痛評分，并于術(shù)后行為學(xué)實驗結(jié)束后取L4-6脊髓進行Western blot方法測定脊髓小膠質(zhì)細胞標記物（OX42）和星形膠質(zhì)細胞標記物（GFAP）的蛋白表達水平及測定IL-1β、IL-6和TNF-α的分泌水平。 結(jié)果：術(shù)后1d，與炎性痛組和生理鹽水組相比，，假手術(shù)組及布比卡因組大鼠MWT增高；累積疼痛評分下降（P＜0.05）；與假手術(shù)組相比，炎性痛組和生理鹽水組大鼠脊髓OX42和GFAP的表達增加（P＜0.05）；與炎性痛組和生理鹽水組相比，布比卡因組大鼠脊髓OX42和GFAP的表達降低（P＜0.05）。與假手術(shù)組相比，炎性痛組和生理鹽水組大鼠脊髓IL-1β、IL-6和TNF-αmRNA表達上調(diào)；與炎性痛組和生理鹽水組相比，布比卡因組大鼠脊髓IL-1β、TNF-αmRNA表達下調(diào)（P＜0.05），而IL-6表達水平在兩組間的差異無統(tǒng)計學(xué)意義（P＞0.05）。 結(jié)論：在炎性疼痛模型中，布比卡因可抑制脊髓膠質(zhì)細胞OX42和GFAP的表達及炎性因子IL-1β、TNF-α釋放。
[Abstract]:Aim: to study the effects of bupivacaine on spinal glial cells and inflammatory factors in formalin inflammatory pain model. Methods: 72 adult male SD rats, weighing 200-220 grams, were randomly divided into 4 groups: sham operation group: right plantar palmar subcutaneous injection of 0.1mL saline; Inflammatory pain group: the right plantar palmar was subcutaneously injected with 2.5% formalin reagent 0.1 mL to establish the formalin inflammatory pain model. In bupivacaine group, the inflammatory pain model was established by injecting 0.125% bupivacaine 40 渭 L into subarachnoid space, and the inflammatory pain model was established by injecting 40 渭 L saline into subarachnoid space with inflammatory pain group. The mechanical foot reflex threshold (MWT) and cumulative pain score were observed before, 1 day, 3 days and 5 days after the establishment of the right plantar palmar inflammatory pain model. After the behavioral experiment, L4-6 spinal cord was taken to measure the protein expression of microglia marker (OX42) and astrocytic marker (GFAP) and IL-1 尾 by Western blot. The secretory levels of IL-6 and TNF- 偽. Results: on the 1st day after operation, compared with the inflammatory pain group and the saline group, the MWT of the sham operation group and the bupivacaine group were increased, the cumulative pain score was decreased (P < 0. 05). Compared with sham operation group, the expression of OX42 and GFAP in spinal cord of rats in inflammatory pain group and saline group was increased (P < 0. 05), and the expression of OX42 and GFAP in spinal cord of bupivacaine group was lower than that of inflammatory pain group and saline group (P < 0. 05). Compared with sham operation group, the expression of IL-1 尾, IL-6 and TNF- 偽 mRNA were up-regulated in inflammatory pain group and saline group. Compared with the inflammatory pain group and the saline group, the expression of IL-1 尾 and TNF- 偽 mRNA in the spinal cord of bupivacaine group was down-regulated (P < 0. 05), but the expression of IL-6 had no significant difference between the two groups (P > 0. 05). Conclusion: bupivacaine can inhibit the expression of OX42 and GFAP and the release of IL-1 尾 and TNF- 偽 in spinal glial cells in inflammatory pain model.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R-332;R614

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