發(fā)布時(shí)間：2019-01-14 07:58

【摘要】：目的研究B細(xì)胞表面TLR2受體在腫瘤來源自噬小體活化B細(xì)胞過程中的作用。方法抗CD43磁珠分選法分離小鼠脾臟B細(xì)胞,用不同質(zhì)量濃度TLR2抗體(0、3、10、30μg/ml)與B細(xì)胞共孵育,再用腫瘤來源自噬小體(DRs)刺激B細(xì)胞72 h,流式細(xì)胞術(shù)檢測B細(xì)胞表面MHCⅡ類分子及共刺激分子CD86的表達(dá)情況;第3天收取上清ELISA檢測細(xì)胞培養(yǎng)上清中IL-6和IL-10的表達(dá);第7天收取上清液,ELISA檢測細(xì)胞培養(yǎng)上清液中Ig M的表達(dá)。結(jié)果與未預(yù)處理的B細(xì)胞組相比,DRs活化TLR2抗體封閉的B細(xì)胞組上調(diào)B細(xì)胞表面MHCⅡ類分子和CD86表達(dá)的作用明顯降低;DRs刺激TLR2抗體封閉B細(xì)胞組分泌IL-6和IL-10的水平顯著降低;DRs刺激TLR2抗體封閉的B細(xì)胞產(chǎn)生Ig M水平顯著低于未預(yù)處理的B細(xì)胞組。結(jié)論 B細(xì)胞的TLR2受體在DRs誘導(dǎo)B細(xì)胞活化、分泌細(xì)胞因子及產(chǎn)生Ig M過程中發(fā)揮了重要作用。
[Abstract]:Objective to study the role of TLR2 receptor on B cell surface in the activation of B cells by tumor derived autophagy. Methods B cells of mouse spleen were isolated by anti CD43 magnetic bead sorting method. B cells were incubated with different concentrations of TLR2 antibody (0 10 030 渭 g/ml). B cells were stimulated by tumor derived autophagy (DRs) for 72 h. The expression of MHC class 鈪，

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