【摘要】：目的探討D-半乳糖(D-gal)致衰小鼠胰腺結(jié)構(gòu)與功能變化。方法 2月齡雄性C57BL/6J小鼠隨機(jī)分為兩組,每組10只。衰老組:小鼠皮下注射D-gal(120mg/kg),每天1次,共42 d;對(duì)照組:小鼠皮下注射等時(shí)與等量生理鹽水。衰老模型建立完成第2天,采外周血測(cè)定空腹血糖(FBG)與空腹胰島素(FINS)水平;稱小鼠體重(g)與胰腺濕重(mg)計(jì)算胰腺臟器指數(shù);石蠟切片,HE染色觀察胰腺光學(xué)顯微鏡下形態(tài);制備胰腺冷凍切片,檢測(cè)衰老相關(guān)β-半乳糖苷酶(SA-β-Gal)染色陽(yáng)性胰腺細(xì)胞的相對(duì)吸光度(RA);免疫組織化學(xué)法觀察胰腺組織晚期糖基化終產(chǎn)物(AGEs)的RA;制備胰腺組織勻漿檢測(cè)超氧化物歧化酶(SOD)、總抗氧化能力(T-AOC)和丙二醛(MDA)的含量。結(jié)果衰老組小鼠FBG顯著升高,FINS水平降低;胰腺濕重和胰腺臟器指數(shù)明顯升高;胰腺光學(xué)顯微鏡下結(jié)構(gòu)未見顯著改變,但胰島內(nèi)單個(gè)有核細(xì)胞所占面積增加;胰腺SA-β-Gal染色陽(yáng)性細(xì)胞數(shù)顯著增加;AGEs陽(yáng)性表達(dá)區(qū)域RA值明顯升高;SOD與T-AOC含量顯著降低,MDA含量顯著升高。結(jié)論 D-gal復(fù)制衰老小鼠可致其胰腺損傷,其機(jī)制與D-gal致胰腺組織細(xì)胞的氧化應(yīng)激損傷有密切關(guān)系。
[Abstract]:Objective to investigate the changes of pancreatic structure and function in aging mice induced by D-galactose (D-gal). Methods two-month old male C57BL/6J mice were randomly divided into two groups with 10 mice in each group. Aging group: mice were subcutaneously injected with D-gal (120mg/kg) once a day for 42 days; control group: mice were subcutaneously injected with the same amount of normal saline. On the second day after the establishment of the aging model, fasting blood glucose (FBG) and fasting insulin (FINS) were measured in peripheral blood, and the pancreatic organ index was calculated by weight (g) and wet weight (mg) of pancreas. Paraffin sections, HE staining to observe the morphology of pancreas under optical microscope; preparation of frozen sections of pancreas to detect relative absorbance of pancreatic cells with SA- 尾-Gal staining The content of total antioxidant activity (T-AOC) of superoxide dismutase (SOD),) and malondialdehyde (MDA) in pancreatic tissue homogenate was determined by immunohistochemical method. Results in aging group, FBG was significantly increased, FINS level was decreased, wet weight of pancreas and pancreatic organ index were significantly increased, the structure of pancreas was not changed significantly under optical microscope, but the area of single nucleated cells in pancreatic islets increased. The number of SA- 尾-Gal positive cells in pancreas was significantly increased, the RA value of AGEs positive region was significantly increased, the contents of SOD and T-AOC were significantly decreased, and MDA content was significantly increased. Conclusion D-gal replication can induce pancreatic injury in aging mice, and its mechanism is closely related to oxidative stress injury of pancreatic tissue cells induced by D-gal.
【作者單位】： 重慶醫(yī)科大學(xué)干細(xì)胞與組織工程研究室組織學(xué)與胚胎學(xué)教研室;
【基金】：國(guó)家自然科學(xué)基金(30973818,81673748)
【分類號(hào)】：R-332;R339.38

【相似文獻(xiàn)】

中國(guó)期刊全文數(shù)據(jù)庫(kù) 前6條

1 崔旭,張炳烈,李文彬,陳桂英;單胺氧化酶B抑制劑對(duì)衰老模型小鼠抗氧化作用的探討[J];中華精神科雜志;1999年03期

2 師迎旭;杜華;鄭國(guó)光;;人胚胎肺成纖維細(xì)胞復(fù)制性衰老模型的建立[J];中國(guó)老年學(xué)雜志;2014年08期

3 文宗曜,宋立川,呂智紅,馬維原,孫大公,竇煥福,肖卉;一種新的紅細(xì)胞衰老模型[J];中國(guó)生物醫(yī)學(xué)工程學(xué)報(bào);1997年02期

4 張石寧,陳明,肖紅,王偉勇,翟書濤;D-半乳糖致衰老模型的研究[J];臨床精神醫(yī)學(xué)雜志;1998年06期

5 金紅;歐芹;王迪迪;;山茱萸多糖對(duì)衰老模型大鼠學(xué)習(xí)記憶能力影響的研究[J];中國(guó)老年學(xué)雜志;2009年12期

6 王志紅;鄧蘭;黃睿;宋朝陽(yáng);賀艷杰;胡亮珊;涂三芳;郭坤元;;D-半乳糖致衰老大鼠骨髓間充質(zhì)干細(xì)胞的特性[J];細(xì)胞與分子免疫學(xué)雜志;2011年03期

中國(guó)碩士學(xué)位論文全文數(shù)據(jù)庫(kù) 前3條

1 靳成偉;生長(zhǎng)停滯特異性-6對(duì)血管平滑肌細(xì)胞衰老影響的研究[D];山東大學(xué);2015年

2 林琳;煙酰胺磷酸核糖轉(zhuǎn)移酶（Nampt）在大鼠骨髓間充質(zhì)干細(xì)胞復(fù)制性衰老中的表達(dá)及意義[D];吉林大學(xué);2015年

3 張亞楠;年齡相關(guān)的小鼠學(xué)習(xí)記憶功能的變化及GDNF的作用[D];曲阜師范大學(xué);2011年

，

本文編號(hào)：2406899