【摘要】：本論文以中國(guó)特有的寄生蟲(chóng)數(shù)據(jù)庫(kù)(ChinaPathDB)的構(gòu)建為依托,收集整理數(shù)據(jù)庫(kù)的基礎(chǔ)數(shù)據(jù),并基于這些數(shù)據(jù)進(jìn)行深入的生物信息學(xué)分析。收集的數(shù)據(jù)類(lèi)型主要有寄生蟲(chóng)相關(guān)的轉(zhuǎn)錄組數(shù)據(jù),對(duì)這些數(shù)據(jù)進(jìn)行了詳細(xì)的歸納總結(jié)。 基于這些基礎(chǔ)數(shù)據(jù),從三個(gè)方面出發(fā),進(jìn)行生物信息學(xué)上的分析。具體來(lái)說(shuō),首先,基于秀麗線蟲(chóng)現(xiàn)有的蛋白蛋白相互作用數(shù)據(jù),預(yù)測(cè)了日本血吸蟲(chóng)和曼氏血吸蟲(chóng)的蛋白相互作用網(wǎng)絡(luò),為寄生蟲(chóng)數(shù)據(jù)庫(kù)的使用者對(duì)蛋白蛋白相互作用的研究帶來(lái)了便利。其次,預(yù)測(cè)了微小隱孢子蟲(chóng)、惡性瘧原蟲(chóng)、日本血吸蟲(chóng)和曼氏血吸蟲(chóng)四個(gè)物種的受體(包括G蛋白偶聯(lián)的受體、核受體、阮病毒蛋白、鉀離子通道),完成了數(shù)據(jù)庫(kù)受體預(yù)測(cè)這一塊的工作。最后,對(duì)日本血吸蟲(chóng)和曼氏血吸蟲(chóng)的血紅蛋白降解途徑進(jìn)行分析,從序列和結(jié)構(gòu)兩個(gè)角度來(lái)分析血吸蟲(chóng)的血紅蛋白降解情況。這部分的研究相對(duì)獨(dú)立,依據(jù)瘧原蟲(chóng)的血紅蛋白降解途徑為基礎(chǔ),預(yù)測(cè)出血吸蟲(chóng)的血紅蛋白降解途徑,并對(duì)降解途徑上的關(guān)鍵酶的保守結(jié)構(gòu)域和底物結(jié)構(gòu)進(jìn)行預(yù)測(cè),研究酶與底物在三維結(jié)構(gòu)上的相互作用關(guān)系。
[Abstract]:Based on the construction of Chinese parasite database (ChinaPathDB), this paper collects and collates the basic data of the database, and makes a deep bioinformatics analysis based on these data. The main types of data collected are parasita-related transcriptional data, which are summarized in detail. Based on these basic data, the bioinformatics analysis is carried out from three aspects. Specifically, first of all, based on the existing protein interaction data, the protein interaction network between Schistosoma japonicum and Schistosoma mansoni is predicted. This facilitates the study of protein interactions by users of parasite databases. Secondly, the receptors of Cryptosporidium minimus, Plasmodium falciparum, Schistosoma japonicum and Schistosoma mansoni were predicted (including G protein-coupled receptor, nuclear receptor, Ruan virus protein, potassium channel). The work of database receptor prediction was completed. Finally, the degradation pathway of hemoglobin of Schistosoma japonicum and Schistosoma mansoni was analyzed, and the degradation of hemoglobin of Schistosoma japonicum was analyzed from the point of view of sequence and structure. Based on the hemoglobin degradation pathway of Plasmodium, the hemoglobin degradation pathway of Schistosoma japonicum was predicted, and the conserved domain and substrate structure of the key enzymes in the degradation pathway were predicted. The interaction between enzyme and substrate in three dimensional structure was studied.
【學(xué)位授予單位】：華東理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R38

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

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