彈性蛋白抗體在豚鼠肺氣腫模型中的表達
發(fā)布時間：2018-12-30 10:29
【摘要】：目的:通過彈性蛋白主動免疫及熏煙制造豚鼠肺氣腫模型,觀察各組模型中彈性蛋白抗體的產(chǎn)生及肺組織的破壞程度。探究彈性蛋白抗體自體免疫對肺氣腫的影響。 方法:將30只雄性英國短毛豚鼠隨機分為3組,分別為主動免疫組,熏煙組,對照組。主動免疫組于皮下多點注射彈性蛋白。熏煙組采用傳統(tǒng)熏煙造模。對照組不做任何處理。造模滿12周后檢測各組豚鼠血清及肺泡灌洗液中的彈性蛋白抗體,并進行肺組織病理形態(tài)觀察,同時測量各組模型的平均肺泡面積及平均肺泡間隔厚度。 結(jié)果:(1)主動免疫組及熏煙組肺組織結(jié)構(gòu)均有不同程度的破壞,光鏡下觀察:主動免疫組肺泡壁有變薄及斷裂,有肺大皰出現(xiàn),可見少量炎性細胞浸潤;熏煙組肺泡結(jié)構(gòu)紊亂,肺泡相互融合成肺大皰,有大量炎性細胞浸潤。(2)與對照組(720.86±139.86μm~2)相比,主動免疫組(977.70±221.69μm~2)及熏煙組(1344.24±309.01μm~2)平均肺泡面積明顯增大(P0.05);且與對照組(3.74±1.50μm)相比,主動免疫組(3.26±1.49μm)及熏煙組(2.30±1.31μm)平均肺泡間隔厚度均變薄(P0.05)。而且,與熏煙組相比,主動免疫組平均肺泡面積較小(P0.05),平均肺泡間隔厚度較厚(P0.05)。(3)與對照組(1.29±0.46,0.25±0.14)相比,主動免疫組(2.14±0.29,1.73±0.33)及熏煙組(2.36±0.26,1.12±0.23)血清及肺泡灌洗液中彈性蛋白抗體均增多(P0.05)。主動免疫組血清及肺泡灌洗液中彈性蛋白抗體無明顯差別(P0.05)。熏煙組血清中彈性蛋白抗體較肺泡灌洗液中明顯增多(P0.05)。主動免疫組與熏煙組血清彈性蛋白抗體無明顯差別(P0.05)。主動免疫組較熏煙組肺泡灌洗液中彈性蛋白抗體增多(P0.05)。 結(jié)論:彈性蛋白主動免疫可以促進豚鼠肺氣腫形成。主動免疫組及熏煙組彈性蛋白抗體均明顯增多,且兩組肺組織均有不同程度破壞,表明彈性蛋白抗體自體免疫對肺氣腫的發(fā)生有一定的促進作用。
[Abstract]:Aim: to establish a guinea pig model of emphysema by active immunization and fumigation of elastin, and to observe the production of elastin antibody and the degree of lung tissue damage in each group. To investigate the effect of self-immunization against elastin antibody on emphysema. Methods: thirty male British short-haired guinea pigs were randomly divided into three groups: active immunization group, fumigation group and control group. The active immunization group was injected with elastin at multiple subcutaneous points. The model of fumigation group was made with traditional fumigation. No treatment was given in the control group. After 12 weeks, the serum and alveolar lavage fluid of guinea pigs were detected for elastin antibodies, and lung histopathology was observed. The mean alveolar area and the mean alveolar septal thickness were measured at the same time. Results: (1) the lung tissue structure of active immunization group and fumigation group were destroyed to some extent. Under light microscope, the alveolar wall of active immunized group became thinner and ruptured, pulmonary bullae appeared, and a small amount of inflammatory cells infiltrated. In the fumigation group, the alveolar structure was disordered, the alveoli fused into bullae, and a large number of inflammatory cells infiltrated. (2) compared with the control group (720.86 鹵139.86 渭 mm2), The mean alveolar area of active immunization group (977.70 鹵221.69 渭 m) and fumigation group (1344.24 鹵309.01 渭 m) was significantly increased (P0.05). Compared with the control group (3.74 鹵1.50 渭 m), the mean thickness of alveolar septum in active immunization group (3.26 鹵1.49 渭 m) and smoke group (2.30 鹵1.31 渭 m) was thinner (P0.05). In addition, compared with the smoking group, the average alveolar area and the thickness of the alveolar septum in the active immunization group were smaller (P0.05), and the thickness of the average alveolar septum (P05 / 3) were higher than those in the control group (1.29 鹵0.460.25 鹵0.14). In the active immunized group (2.14 鹵0.29) 1.73 鹵0.33 and the fumigated group (2.36 鹵0.261.12 鹵0.23), the serum and alveolar lavage fluid antibodies were increased (P0.05). There was no significant difference in serum and alveolar lavage fluid (P 0.05). The level of serum elastin antibody in fumigation group was significantly higher than that in alveolar lavage fluid (P0.05). There was no significant difference in serum elastin antibody between active immunization group and fumigation group (P0.05). The level of elastin antibody in alveolar lavage fluid of active immunization group was higher than that of fumigation group (P0.05). Conclusion: active immunization with elastin can promote emphysema formation in guinea pigs. Both active immunization group and fumigation group increased the level of elastin antibody, and the lung tissues of both groups were damaged to some extent, which indicated that the autoimmunity of elastin antibody could promote the occurrence of emphysema.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R563.3;R-332
本文編號：2395451
[Abstract]:Aim: to establish a guinea pig model of emphysema by active immunization and fumigation of elastin, and to observe the production of elastin antibody and the degree of lung tissue damage in each group. To investigate the effect of self-immunization against elastin antibody on emphysema. Methods: thirty male British short-haired guinea pigs were randomly divided into three groups: active immunization group, fumigation group and control group. The active immunization group was injected with elastin at multiple subcutaneous points. The model of fumigation group was made with traditional fumigation. No treatment was given in the control group. After 12 weeks, the serum and alveolar lavage fluid of guinea pigs were detected for elastin antibodies, and lung histopathology was observed. The mean alveolar area and the mean alveolar septal thickness were measured at the same time. Results: (1) the lung tissue structure of active immunization group and fumigation group were destroyed to some extent. Under light microscope, the alveolar wall of active immunized group became thinner and ruptured, pulmonary bullae appeared, and a small amount of inflammatory cells infiltrated. In the fumigation group, the alveolar structure was disordered, the alveoli fused into bullae, and a large number of inflammatory cells infiltrated. (2) compared with the control group (720.86 鹵139.86 渭 mm2), The mean alveolar area of active immunization group (977.70 鹵221.69 渭 m) and fumigation group (1344.24 鹵309.01 渭 m) was significantly increased (P0.05). Compared with the control group (3.74 鹵1.50 渭 m), the mean thickness of alveolar septum in active immunization group (3.26 鹵1.49 渭 m) and smoke group (2.30 鹵1.31 渭 m) was thinner (P0.05). In addition, compared with the smoking group, the average alveolar area and the thickness of the alveolar septum in the active immunization group were smaller (P0.05), and the thickness of the average alveolar septum (P05 / 3) were higher than those in the control group (1.29 鹵0.460.25 鹵0.14). In the active immunized group (2.14 鹵0.29) 1.73 鹵0.33 and the fumigated group (2.36 鹵0.261.12 鹵0.23), the serum and alveolar lavage fluid antibodies were increased (P0.05). There was no significant difference in serum and alveolar lavage fluid (P 0.05). The level of serum elastin antibody in fumigation group was significantly higher than that in alveolar lavage fluid (P0.05). There was no significant difference in serum elastin antibody between active immunization group and fumigation group (P0.05). The level of elastin antibody in alveolar lavage fluid of active immunization group was higher than that of fumigation group (P0.05). Conclusion: active immunization with elastin can promote emphysema formation in guinea pigs. Both active immunization group and fumigation group increased the level of elastin antibody, and the lung tissues of both groups were damaged to some extent, which indicated that the autoimmunity of elastin antibody could promote the occurrence of emphysema.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R563.3;R-332
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相關(guān)期刊論文 前1條
1 許三林,吳人亮,陳春蓮,郝春榮;上皮鈣粘附素在吸煙小鼠呼吸道上皮損傷修復(fù)中表達的研究[J];中華結(jié)核和呼吸雜志;1999年07期
，本文編號：2395451
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