【摘要】：目的:血管內(nèi)皮細(xì)胞衰老是動脈粥樣硬化發(fā)生的病理生理機制之一。本研究旨在探討人參皂苷Rb1延緩人臍靜脈內(nèi)皮細(xì)胞(HUVECs)早熟性衰老與窖蛋白1(caveolin-1)表達(dá)的關(guān)系,為延緩HUVECs衰老提供新的靶點。方法:建立60μmol/L過氧化氫(H_2O_2)誘導(dǎo)的HUVECs早熟性衰老模型,根據(jù)細(xì)胞形態(tài)學(xué)的變化、衰老相關(guān)β-半乳糖苷酶(SA-β-Gal)染色陽性率和細(xì)胞周期評估內(nèi)皮細(xì)胞衰老,采用Western blot和激光共聚焦顯微成像的方法檢測caveolin-1的變化,觀察人參皂苷Rb1對HUVECs衰老的作用及其相關(guān)的分子機制。結(jié)果:60μmol/L H_2O_2可成功地誘導(dǎo)內(nèi)皮細(xì)胞衰老,早熟性衰老的HUVECs體積變大,SA-β-Gal活性明顯增加,細(xì)胞發(fā)生G_1期阻滯,細(xì)胞增殖受抑制,caveolin-1表達(dá)增多。與H_2O_2處理組相比,人參皂苷Rb1預(yù)處理延緩HUVECs早熟性衰老,SA-β-Gal染色陽性細(xì)胞百分比降低,G_0/G_1期細(xì)胞比例下降,caveolin-1表達(dá)減少。結(jié)論:人參皂苷Rb1可通過抑制caveolin-1的表達(dá)延緩H_2O_2誘導(dǎo)的HUVECs早熟性衰老。
[Abstract]:Objective: aging of vascular endothelial cells is one of the pathophysiological mechanisms of atherosclerosis. The aim of this study was to investigate the relationship between ginsenoside Rb1 and the expression of cellar protein 1 (caveolin-1) in human umbilical vein endothelial cells (HUVECs), and to provide a new target for delaying HUVECs senescence. Methods: 60 渭 mol/L hydrogen peroxide (H_2O_2) induced HUVECs precocious senescence model was established. Senescence associated 尾 -galactosidase (SA- 尾 -galactosidase) staining and cell cycle were used to evaluate the senescence of endothelial cells. Western blot and confocal laser microscopy were used to detect the changes of caveolin-1. To observe the effect of ginsenoside Rb1 on HUVECs senescence and its molecular mechanism. Results: 60 渭 mol/L H_2O_2 could successfully induce endothelial cell senescence. The HUVECs volume of precocious senescence increased, the activity of SA- 尾-Gal increased significantly, cell G-1 arrest occurred, cell proliferation was inhibited and caveolin-1 expression increased. Compared with H_2O_2 treatment group, ginsenoside Rb1 pretreatment delayed HUVECs precocious senescence, decreased the percentage of SA- 尾-Gal positive cells, decreased the percentage of G_0/G_1 phase cells, and decreased caveolin-1 expression. Conclusion: ginsenoside Rb1 can delay HUVECs senescence induced by H_2O_2 by inhibiting the expression of caveolin-1.
【作者單位】： 中山大學(xué)附屬第三醫(yī)院心血管內(nèi)科;中山大學(xué)中西醫(yī)結(jié)合研究所;
【基金】：國家自然科學(xué)基金資助項目(No.81370447) 廣東省自然科學(xué)基金博士啟動項目(No.2015A030310048)
【分類號】：R363

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