骨通貼膏對甲醛疼痛模型大鼠的鎮(zhèn)痛作用及機(jī)制研究
發(fā)布時(shí)間：2018-12-17 21:30
【摘要】：目的:研究骨通貼膏對甲醛疼痛模型大鼠的鎮(zhèn)痛作用及機(jī)制。方法:60只SD大鼠隨機(jī)均分為空白組,模型組,骨通貼膏低、中、高劑量組(0.594、1.188、2.376 g/貼,含生藥0.48、0.96、1.92 g)與醋酸潑尼松組(ig給藥,0.005 4 g/kg,外貼基質(zhì)),采用甲醛法復(fù)制大鼠疼痛模型,造模后立即給藥。采用電子壓痛儀測定給藥1、2、3、4、6 h后各組大鼠痛閾值;給藥6 h后,于腹主動(dòng)脈取血0.3 m L并處死大鼠,采用酶聯(lián)免疫吸附試驗(yàn)(ELISA)測定大鼠血漿中β-內(nèi)啡肽(β-EP)、前列腺素E2(PGE2)含量,分光光度法測定大鼠血清、炎癥組織中一氧化氮(NO)含量,放射免疫法檢測大鼠血清、炎癥組織及腦組織中P物質(zhì)含量。結(jié)果:與造模前比較,模型組大鼠各個(gè)時(shí)段痛閾值均降低(P0.05或P0.01);與空白組比較,模型組大鼠PGE2、NO、炎癥組織和腦組織中P物質(zhì)含量顯著升高(P0.05或P0.01);與模型組比較,骨通貼膏各劑量組大鼠給藥后對應(yīng)時(shí)間點(diǎn)的痛閾值升高,PGE2、炎癥組織與腦組織中P物質(zhì)含量降低(P0.05或P0.01),骨通貼膏中、高劑量組β-EP含量升高、炎癥組織中NO含量降低(P0.05或P0.01),骨通貼膏高劑量組血清中NO含量降低(P0.05)。結(jié)論:骨通貼膏具有一定的鎮(zhèn)痛抗炎作用,其作用機(jī)制可能與降低PGE2、NO、P物質(zhì)含量,升高β-EP含量有關(guān)。
[Abstract]:Objective: to study the analgesic effect and mechanism of Gutong plaster on formaldehyde pain model rats. Methods: sixty SD rats were randomly divided into blank group, model group, low, medium and high dose group (0.594L 1.1880.376g / plaster, containing 0.480.96g) and prednisone acetate group (ig, 0.005 g / kg). The rat model of pain was made by formaldehyde method and the drug was administered immediately after making the model. The pain threshold of rats in each group was determined by electronic tenderness apparatus after 6 hours of administration. After 6 hours of administration, blood samples were taken from abdominal aorta and the rats were killed. The plasma 尾-EP), prostaglandin E 2 (PGE2) was determined by enzyme linked immunosorbent assay (ELISA). The content of nitric oxide (NO) in serum and inflammatory tissue of rats was determined by spectrophotometry, and substance P in serum, inflammatory tissue and brain tissue was detected by radioimmunoassay. Results: compared with the model group, the pain threshold of the model group was significantly lower than that of the control group (P0.05 or P0.01). Compared with the blank group, the content of substance P in the inflammatory tissue and brain tissue of the model group was significantly higher than that in the control group (P0.05 or P0.01). Compared with the model group, the pain threshold at the corresponding time points was increased, the content of substance P in the inflammatory tissue and brain tissue of PGE2, was decreased (P0.05 or P0.01), and in the Gutong plaster, the pain threshold was increased at the corresponding time point after the administration of Gutong plaster (P0.05 or P0.01). The content of 尾-EP in high dose group increased, the content of NO in inflammatory tissue decreased (P0.05 or P0.01), and the content of NO in serum of high dose group of Gutong plaster decreased (P0.05). Conclusion: Gutong plaster has a certain analgesic and anti-inflammatory effect, and its mechanism may be related to decreasing the content of PGE2,NO,P and increasing the content of 尾-EP.
【作者單位】： 廣州中醫(yī)藥大學(xué)科技產(chǎn)業(yè)園;廣州中醫(yī)藥大學(xué)中藥學(xué)院;桂林華潤天和藥業(yè)有限公司;廣東新南方青蒿藥業(yè)有限公司;
【基金】：廣東省省級科技計(jì)劃項(xiàng)目(No.2013B090800024,2014B040404066)
【分類號】：R285.5;R-332
本文編號：2384822
[Abstract]:Objective: to study the analgesic effect and mechanism of Gutong plaster on formaldehyde pain model rats. Methods: sixty SD rats were randomly divided into blank group, model group, low, medium and high dose group (0.594L 1.1880.376g / plaster, containing 0.480.96g) and prednisone acetate group (ig, 0.005 g / kg). The rat model of pain was made by formaldehyde method and the drug was administered immediately after making the model. The pain threshold of rats in each group was determined by electronic tenderness apparatus after 6 hours of administration. After 6 hours of administration, blood samples were taken from abdominal aorta and the rats were killed. The plasma 尾-EP), prostaglandin E 2 (PGE2) was determined by enzyme linked immunosorbent assay (ELISA). The content of nitric oxide (NO) in serum and inflammatory tissue of rats was determined by spectrophotometry, and substance P in serum, inflammatory tissue and brain tissue was detected by radioimmunoassay. Results: compared with the model group, the pain threshold of the model group was significantly lower than that of the control group (P0.05 or P0.01). Compared with the blank group, the content of substance P in the inflammatory tissue and brain tissue of the model group was significantly higher than that in the control group (P0.05 or P0.01). Compared with the model group, the pain threshold at the corresponding time points was increased, the content of substance P in the inflammatory tissue and brain tissue of PGE2, was decreased (P0.05 or P0.01), and in the Gutong plaster, the pain threshold was increased at the corresponding time point after the administration of Gutong plaster (P0.05 or P0.01). The content of 尾-EP in high dose group increased, the content of NO in inflammatory tissue decreased (P0.05 or P0.01), and the content of NO in serum of high dose group of Gutong plaster decreased (P0.05). Conclusion: Gutong plaster has a certain analgesic and anti-inflammatory effect, and its mechanism may be related to decreasing the content of PGE2,NO,P and increasing the content of 尾-EP.
【作者單位】： 廣州中醫(yī)藥大學(xué)科技產(chǎn)業(yè)園;廣州中醫(yī)藥大學(xué)中藥學(xué)院;桂林華潤天和藥業(yè)有限公司;廣東新南方青蒿藥業(yè)有限公司;
【基金】：廣東省省級科技計(jì)劃項(xiàng)目(No.2013B090800024,2014B040404066)
【分類號】：R285.5;R-332
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1 夏菁;謝媛媛;張宗璇;張延惠;王義明;梁瓊麟;羅國安;;骨通貼膏指紋圖譜及化學(xué)成分研究[J];中成藥;2014年03期
，本文編號：2384822
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