【摘要】：目的：研究缺氧條件下15-羥基二十碳四烯酸（15-HETE）對(duì)兔肺動(dòng)脈平滑肌細(xì)胞增殖的影響 方法：培養(yǎng)日本大耳白兔肺動(dòng)脈平滑肌細(xì)胞（PASMC）,Western blotting檢測(cè)Phospho-ERK1/2、ERK1/2、Phospho-p38MAPK及p38MAPK的表達(dá)。通過應(yīng)用四噻唑藍(lán)比色法（MTT）及Brdu法觀察MEK阻斷劑U0126及p38MAPK阻斷劑SB202190以及15-HETE在缺氧情況下對(duì)肺動(dòng)脈平滑肌細(xì)胞增殖的影響及可能機(jī)制。 結(jié)果：缺氧使兔肺動(dòng)脈平滑肌細(xì)胞發(fā)生增殖并且上調(diào)15-LO的表達(dá)，，15-HETE可促進(jìn)兔肺動(dòng)脈平滑肌細(xì)胞增殖并且激活Phospho-ERK1/2的表達(dá)，ERK1/2未發(fā)生明顯變化。加入MEK阻斷劑U0126后細(xì)胞的增殖作用受到了阻斷。 結(jié)論：缺氧條件下15-HETE促進(jìn)兔肺動(dòng)脈平滑肌細(xì)胞增殖與MAPK家族中的ERK1/2信號(hào)轉(zhuǎn)導(dǎo)通路有關(guān)。
[Abstract]:Objective: to study the effect of 15-hydroxy-icosapentaenoic acid (15-HETE) on the proliferation of rabbit pulmonary artery smooth muscle cells under hypoxia. Methods: Phospho-ERK1/2, was detected by (PASMC), Western blotting in cultured rabbit pulmonary artery smooth muscle cells. Expression of ERK1/2,Phospho-p38MAPK and p38MAPK. The effects of MEK blocker U0126, p38MAPK blocker SB202190 and 15-HETE on the proliferation of pulmonary artery smooth muscle cells under hypoxia were observed by (MTT) and Brdu assay with tetrathiazolyl blue colorimetry. Results: hypoxia induced proliferation of rabbit pulmonary artery smooth muscle cells and up-regulated the expression of 15-LO. 15-HETE promoted the proliferation of rabbit pulmonary artery smooth muscle cells and activated the expression of Phospho-ERK1/2. ERK1/2 did not change significantly. The proliferation of U0126 cells was blocked by the addition of MEK blocker U0126. Conclusion: the proliferation of rabbit pulmonary artery smooth muscle cells stimulated by 15-HETE under hypoxia is related to the ERK1/2 signal transduction pathway in the MAPK family.
【學(xué)位授予單位】：哈爾濱醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R329.2

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